Electrochemical quantitative determination of sertraline in pharmaceutical formulation using a gold electrode in bicarbonate solution

Abstract

The electrochemical characterization of sertraline standard at gold electrode was at first performed by cyclic voltammetry measurements (CV) in pH 8.4 bicarbonate buffer. Then Au electrode was evaluated for the quantitative determination of sertraline using square wave voltammetry (SWV). Namely, (2-hydroxypropyl)-β-cyclodextrin (HPβCD) sertraline inclusion complexe was employed to enhance the electrode sensitivity of the drug determination. Using the proposed SWV technique, the anodic current peak of sertraline oxidation was linear within a concentration range of 0.1–0.5 μM with a limit of detection (LOD) of 2.0  10-8 M and a limit of quantification (LOQ) of 6.7  10-8 M. In the case of inclusion complex of the sertraline with HPβCD, a good linearity range of 0.1–0.9 μM was obtained with a LOD of 2.6  10-8 M and a LOQ of 8.8  10-8 μM. Comparing the regression equations, it can be concluded that the sensitivity in the presence of inclusion complex can be up to 5 times higher. The applicability of the developed method was confirmed by the analysis of this drug in pharmaceutical formulation.

Elektrohemijska karakterizacija standarda sertralina je urađena cikličnom voltametrijom(CV) u pH 8.4 bicarbonatnom puferu. Elektroda od zlata je zatim testirana za kvantitativno određivanje sertralina koristeći voltametriju sa pravougaonim impulsima (SWV). Inkluzioni kompleks (2-hydroxypropyl)-β-cyclodextrin (HPβCD) sertralina je testiran u cilju poboljšanja osetljivosti electrode za kvantitativno određivanje leka. SWV tehnikom je pokazano da je anodni strujni vrh oksidacije sertralina linearan u opsegu koncentracija 0.1–0.5 μM uz granicu detekcije (LOD) od 2.0  10-8 M i granicu kvantifikacije (LOQ) od 6.7  10-8 M. Inkluzioni kompleks sertralina sa HPβCD je ispoljio linearnost u opsegu koncentracija od 0.1–0.9 μM uz LOD of 2.6  10-8 M i LOQ od 8.8  10-8 μM. Analizom eksperimentalnih podataka može se zaklučiti da je osetljivost electrode od zlata za određivanje sertraline porasla više od pet puta kada je lek vezan u inkluzioni kompleks. Primenljivost razvijene metode je potvrđena uspešnim određivanjem leka u farmaceutskim oblicima.