Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis
Аутори
Arsenijević, DraganaStojanović, Bojana
Milovanović, Jelena
Arsenijević, Aleksandar
Simić, Miloš
Pergal, Marija
Kodranov, Igor
Cvetković, Olga
Vojvodić, Danilo
Ristanović, Elizabeta
Manojlović, Dragan
Milovanović, Marija
Arsenijević, Nebojša
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysul-fides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while... the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, charac-terized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.
Кључне речи:
Anti-inflammatory activity / ConA hepatitis / Dipropyl polysulfides / Hepatoprotective effectsИзвор:
Nutrients, 2021, 13, 3, 1-15Издавач:
- MDPI
Финансирање / пројекти:
- Молекулске детерминанте урођене имуности у аутоимунским болестима и канцерогенези (RS-MESTD-Basic Research (BR or ON)-175069)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
- Порозни материјали на бази оксида у заштити животне средине од генотоксичних супстанци (RS-MESTD-Basic Research (BR or ON)-172018)
- The Serbian bilateral project with PR China (06/2018)
- The Faculty of Medical Sciences, University of Kragujevac - MP 01/19
- The Faculty of Medical Sciences, University of Kragujevac - JP 22/19
DOI: 10.3390/nu13031022
ISSN: 2072-6643
PubMed: 33809904
WoS: 000633943600001
Scopus: 2-s2.0-85102752250
Институција/група
IHTMTY - JOUR AU - Arsenijević, Dragana AU - Stojanović, Bojana AU - Milovanović, Jelena AU - Arsenijević, Aleksandar AU - Simić, Miloš AU - Pergal, Marija AU - Kodranov, Igor AU - Cvetković, Olga AU - Vojvodić, Danilo AU - Ristanović, Elizabeta AU - Manojlović, Dragan AU - Milovanović, Marija AU - Arsenijević, Nebojša PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4835 AB - The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysul-fides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, charac-terized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases. PB - MDPI T2 - Nutrients T1 - Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis VL - 13 IS - 3 SP - 1 EP - 15 DO - 10.3390/nu13031022 ER -
@article{ author = "Arsenijević, Dragana and Stojanović, Bojana and Milovanović, Jelena and Arsenijević, Aleksandar and Simić, Miloš and Pergal, Marija and Kodranov, Igor and Cvetković, Olga and Vojvodić, Danilo and Ristanović, Elizabeta and Manojlović, Dragan and Milovanović, Marija and Arsenijević, Nebojša", year = "2021", abstract = "The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysul-fides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, charac-terized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.", publisher = "MDPI", journal = "Nutrients", title = "Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis", volume = "13", number = "3", pages = "1-15", doi = "10.3390/nu13031022" }
Arsenijević, D., Stojanović, B., Milovanović, J., Arsenijević, A., Simić, M., Pergal, M., Kodranov, I., Cvetković, O., Vojvodić, D., Ristanović, E., Manojlović, D., Milovanović, M.,& Arsenijević, N.. (2021). Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis. in Nutrients MDPI., 13(3), 1-15. https://doi.org/10.3390/nu13031022
Arsenijević D, Stojanović B, Milovanović J, Arsenijević A, Simić M, Pergal M, Kodranov I, Cvetković O, Vojvodić D, Ristanović E, Manojlović D, Milovanović M, Arsenijević N. Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis. in Nutrients. 2021;13(3):1-15. doi:10.3390/nu13031022 .
Arsenijević, Dragana, Stojanović, Bojana, Milovanović, Jelena, Arsenijević, Aleksandar, Simić, Miloš, Pergal, Marija, Kodranov, Igor, Cvetković, Olga, Vojvodić, Danilo, Ristanović, Elizabeta, Manojlović, Dragan, Milovanović, Marija, Arsenijević, Nebojša, "Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis" in Nutrients, 13, no. 3 (2021):1-15, https://doi.org/10.3390/nu13031022 . .