The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysul-fides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while... the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, charac-terized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.
TY - JOUR
AU - Arsenijević, Dragana
AU - Stojanović, Bojana
AU - Milovanović, Jelena
AU - Arsenijević, Aleksandar
AU - Simić, Miloš
AU - Pergal, Marija
AU - Kodranov, Igor
AU - Cvetković, Olga
AU - Vojvodić, Danilo
AU - Ristanović, Elizabeta
AU - Manojlović, Dragan
AU - Milovanović, Marija
AU - Arsenijević, Nebojša
PY - 2021
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4835
AB - The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysul-fides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, charac-terized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.
PB - MDPI
T2 - Nutrients
T1 - Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis
VL - 13
IS - 3
SP - 1
EP - 15
DO - 10.3390/nu13031022
ER -
@article{
author = "Arsenijević, Dragana and Stojanović, Bojana and Milovanović, Jelena and Arsenijević, Aleksandar and Simić, Miloš and Pergal, Marija and Kodranov, Igor and Cvetković, Olga and Vojvodić, Danilo and Ristanović, Elizabeta and Manojlović, Dragan and Milovanović, Marija and Arsenijević, Nebojša",
year = "2021",
abstract = "The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysul-fides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, charac-terized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.",
publisher = "MDPI",
journal = "Nutrients",
title = "Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis",
volume = "13",
number = "3",
pages = "1-15",
doi = "10.3390/nu13031022"
}
Arsenijević, D., Stojanović, B., Milovanović, J., Arsenijević, A., Simić, M., Pergal, M., Kodranov, I., Cvetković, O., Vojvodić, D., Ristanović, E., Manojlović, D., Milovanović, M.,& Arsenijević, N.. (2021). Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis. in Nutrients
MDPI., 13(3), 1-15.
https://doi.org/10.3390/nu13031022
Arsenijević D, Stojanović B, Milovanović J, Arsenijević A, Simić M, Pergal M, Kodranov I, Cvetković O, Vojvodić D, Ristanović E, Manojlović D, Milovanović M, Arsenijević N. Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis. in Nutrients. 2021;13(3):1-15.
doi:10.3390/nu13031022 .
