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dc.creatorKrunić, Matija
dc.creatorRistić, Biljana
dc.creatorBošnjak, Mihajlo
dc.creatorPaunović, Verica
dc.creatorTovilović-Kovačević, Gordana
dc.creatorZogović, Nevena
dc.creatorMirčić, Aleksandar
dc.creatorMarković, Zoran
dc.creatorTodorović-Marković, Biljana
dc.creatorJovanović, Svetlana
dc.creatorKleut, Duška
dc.creatorMojović, Miloš
dc.creatorNakarada, Đura
dc.creatorMarković, Olivera
dc.creatorVuković, Irena
dc.creatorHarhaji-Trajković, Ljubica
dc.creatorTrajković, Vladimir
dc.date.accessioned2021-10-27T13:26:43Z
dc.date.available2022-10-20
dc.date.issued2021
dc.identifier.issn0891-5849
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/4818
dc.description.abstractWe investigated the ability of graphene quantum dot (GQD) nanoparticles to protect SH-SY5Y human neuroblastoma cells from oxidative/nitrosative stress induced by iron-nitrosyl complex sodium nitroprusside (SNP).GQD reduced SNP cytotoxicity by preventing mitochondrial depolarization, caspase-2 activation, and subsequent apoptotic death. Although GQD diminished the levels of nitric oxide (NO) in SNP-exposed cells, NO scavengers displayed only a slight protective effect, suggesting that NO quenching was not the main protective mechanism of GQD. GQD also reduced SNP-triggered increase in the intracellular levels of hydroxyl radical (•OH), superoxide anion (O2•- ), and lipid peroxidation. Nonselective antioxidants, •OH scavenging, and iron chelators, but not superoxide dismutase, mimicked GQD cytoprotective activity, indicating that GQD protect cells by neutralizing •OH generated in the presence of SNP-released iron. Cellular internalization of GQD was required for optimal protection, since a removal of extracellular GQD by extensive washing only partly diminished their protective effect. Moreover, GQD cooperated with SNP to induce autophagy, as confirmed by the inhibition of autophagylimiting Akt/PRAS40/mTOR signaling and increase in autophagy gene transcription, protein levels of proautophagic beclin-1 and LC3-II, formation of autophagic vesicles, and degradation of autophagic target p62. The antioxidant activity of GQD was not involved in autophagy induction, as antioxidants N-acetylcysteine and dimethyl sulfoxide failed to stimulate autophagy in SNP-exposed cells. Pharmacological inhibitors of early (wortmannin, 3-methyladenine) or late stages of autophagy (NH4Cl) efficiently reduced the protective effect of GQD. Therefore, the ability of GQD to prevent the in vitro neurotoxicity of SNP depends on both •OH/NO scavenging and induction of cytoprotective autophagy.sr
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200110/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200017/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200146/RS//sr
dc.relation.isversionofhttps://doi.org/10.1016/j.freeradbiomed.2021.10.025
dc.relation.isversionofhttps://cer.ihtm.bg.ac.rs/handle/123456789/4812
dc.rightsembargoedAccesssr
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceFree Radical Biology and Medicinesr
dc.subjectGraphene quantum dotssr
dc.subjectSodium nitroprussidesr
dc.subjectNeurotoxicitysr
dc.subjectOxidative stresssr
dc.subjectHydroxyl radicalsr
dc.subjectNitric oxidesr
dc.subjectAutophagysr
dc.titleGraphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic deathsr
dc.typearticlesr
dc.rights.licenseBY-NC-NDsr
dcterms.abstractНакарада, Ђура; Марковић, Зоран; Мојовић, Милош; Клеут, Душка; Јовановић, Светлана; Тодоровић-Марковић, Биљана; Крунић, Матија; Ристић, Биљана; Бошњак, Михајло; Пауновић, Верица; Товиловић-Ковачевић, Гордана; Марковић, Оливера; Вуковић, Ирена; Хархаји-Трајковић, Љубица; Трајковић, Владимир; Мирчић, Aлександар; Зоговић, Невена;
dc.citation.volume177
dc.citation.spage167
dc.citation.epage180
dc.citation.rankM21~
dc.description.otherThis is the peer-reviewed version of the article: Matija Krunić, Biljana Ristić, Mihajlo Bošnjak, Verica Paunović, Gordana Tovilović- Kovacević, Nevena Zogović, Aleksandar Mirčić, Zoran Marković, Biljana Todorović- Marković, Svetlana Jovanović, Duška Kleut, Miloš Mojović, Đura Nakarada, Olivera Marković, Irena Vuković, Ljubica Harhaji-Trajković, Vladimir Trajković, Graphene quantum dot antioxidant and proautophagic actions protect SH-SY5Y neuroblastoma cells from oxidative stress-mediated apoptotic death, Free Radical Biology and Medicine, 2021, 177, 167-180, doi: [https://doi.org/10.1016/j.freeradbiomed.2021.10.025]
dc.description.otherThe published version: [https://cer.ihtm.bg.ac.rs/handle/123456789/4812]
dc.identifier.doi10.1016/j.freeradbiomed.2021.10.025
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/20989/acc_1-s2.0-S0891584921007760-main.pdf
dc.identifier.wos000717740300004
dc.type.versionacceptedVersionsr


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