Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks
Samo za registrovane korisnike
2006
Autori
Radulovic, ZeljkoMilutinovic, Marija
Andelković, Marko
Vujčić, Zoran
Tomanovic, Snezana
Božić, Nataša
Marinkovic, Dragoslav
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Ixodes ricinus Linnaeus (Acari: Ixodidae) ticks are vectors of numerous infectious diseases in humans and animals. The allozyme variability of MDH and alpha-Gpdh was detected by native polyacrylamide gel electrophoresis in I. ricinus natural populations in three localities in Serbia. Four alleles of Mdh locus (MDH 1, MDH 2, MDH 3 and MDH X) and four alleles of alpha-Gpdh locus (VS, S, F and VF) were detected. Interpopulation differences in Mdh and alpha-Gpdh allele frequencies were statistically insignificant. Significant difference in alpha-Gpdh allele frequencies between mates and females was recorded in the largest sample only. Differences in allele frequencies, detected between borreliae-infected and uninfected I. ricinus ticks, were close to the level of statistical significance, especially for alpha-Gpdh locus. Clear significant difference appeared in females when sexes were tested separatelly (P = 0.037). It is interesting that genotypes containing rarer alleles (MDH 1 and S) we...re infected in higher proportion in comparison to other genotypes. Our results point towards a possible role of Mdh and alpha-Gpdh loci in I. ricinus ticks in the determination of energy requirements for host seeking. Sex differences in alpha-Gpdh allele frequencies suggest that selective pressure, concerning efficiency of reserve materials utilisation, points to alpha-Gpdh rather than to Mdh locus.
Ključne reči:
Ixodes ricinus / Borrelia burgdorferi sensu lato / Lyme disease / allozyme variability / alpha-glycerophosphate dehydrogenase / malate dehydrogenaseIzvor:
Experimental and Applied Acarology, 2006, 40, 2, 113-121Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Infekcije intercelularnim mikroorganizmima rastućeg značaja: transmisija, odnos patogen-domaćin, molekularna epidemiologija i klinički značaj (RS-MESTD-MPN2006-2010-145002)
DOI: 10.1007/s10493-006-9028-z
ISSN: 0168-8162
PubMed: 17072538
WoS: 000243152700003
Scopus: 2-s2.0-33751290652
Institucija/grupa
IHTMTY - JOUR AU - Radulovic, Zeljko AU - Milutinovic, Marija AU - Andelković, Marko AU - Vujčić, Zoran AU - Tomanovic, Snezana AU - Božić, Nataša AU - Marinkovic, Dragoslav PY - 2006 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4669 AB - Ixodes ricinus Linnaeus (Acari: Ixodidae) ticks are vectors of numerous infectious diseases in humans and animals. The allozyme variability of MDH and alpha-Gpdh was detected by native polyacrylamide gel electrophoresis in I. ricinus natural populations in three localities in Serbia. Four alleles of Mdh locus (MDH 1, MDH 2, MDH 3 and MDH X) and four alleles of alpha-Gpdh locus (VS, S, F and VF) were detected. Interpopulation differences in Mdh and alpha-Gpdh allele frequencies were statistically insignificant. Significant difference in alpha-Gpdh allele frequencies between mates and females was recorded in the largest sample only. Differences in allele frequencies, detected between borreliae-infected and uninfected I. ricinus ticks, were close to the level of statistical significance, especially for alpha-Gpdh locus. Clear significant difference appeared in females when sexes were tested separatelly (P = 0.037). It is interesting that genotypes containing rarer alleles (MDH 1 and S) were infected in higher proportion in comparison to other genotypes. Our results point towards a possible role of Mdh and alpha-Gpdh loci in I. ricinus ticks in the determination of energy requirements for host seeking. Sex differences in alpha-Gpdh allele frequencies suggest that selective pressure, concerning efficiency of reserve materials utilisation, points to alpha-Gpdh rather than to Mdh locus. PB - Springer, Dordrecht T2 - Experimental and Applied Acarology T1 - Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks VL - 40 IS - 2 SP - 113 EP - 121 DO - 10.1007/s10493-006-9028-z ER -
@article{ author = "Radulovic, Zeljko and Milutinovic, Marija and Andelković, Marko and Vujčić, Zoran and Tomanovic, Snezana and Božić, Nataša and Marinkovic, Dragoslav", year = "2006", abstract = "Ixodes ricinus Linnaeus (Acari: Ixodidae) ticks are vectors of numerous infectious diseases in humans and animals. The allozyme variability of MDH and alpha-Gpdh was detected by native polyacrylamide gel electrophoresis in I. ricinus natural populations in three localities in Serbia. Four alleles of Mdh locus (MDH 1, MDH 2, MDH 3 and MDH X) and four alleles of alpha-Gpdh locus (VS, S, F and VF) were detected. Interpopulation differences in Mdh and alpha-Gpdh allele frequencies were statistically insignificant. Significant difference in alpha-Gpdh allele frequencies between mates and females was recorded in the largest sample only. Differences in allele frequencies, detected between borreliae-infected and uninfected I. ricinus ticks, were close to the level of statistical significance, especially for alpha-Gpdh locus. Clear significant difference appeared in females when sexes were tested separatelly (P = 0.037). It is interesting that genotypes containing rarer alleles (MDH 1 and S) were infected in higher proportion in comparison to other genotypes. Our results point towards a possible role of Mdh and alpha-Gpdh loci in I. ricinus ticks in the determination of energy requirements for host seeking. Sex differences in alpha-Gpdh allele frequencies suggest that selective pressure, concerning efficiency of reserve materials utilisation, points to alpha-Gpdh rather than to Mdh locus.", publisher = "Springer, Dordrecht", journal = "Experimental and Applied Acarology", title = "Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks", volume = "40", number = "2", pages = "113-121", doi = "10.1007/s10493-006-9028-z" }
Radulovic, Z., Milutinovic, M., Andelković, M., Vujčić, Z., Tomanovic, S., Božić, N.,& Marinkovic, D.. (2006). Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks. in Experimental and Applied Acarology Springer, Dordrecht., 40(2), 113-121. https://doi.org/10.1007/s10493-006-9028-z
Radulovic Z, Milutinovic M, Andelković M, Vujčić Z, Tomanovic S, Božić N, Marinkovic D. Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks. in Experimental and Applied Acarology. 2006;40(2):113-121. doi:10.1007/s10493-006-9028-z .
Radulovic, Zeljko, Milutinovic, Marija, Andelković, Marko, Vujčić, Zoran, Tomanovic, Snezana, Božić, Nataša, Marinkovic, Dragoslav, "Allozyme polymorphism of Mdh and alpha-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks" in Experimental and Applied Acarology, 40, no. 2 (2006):113-121, https://doi.org/10.1007/s10493-006-9028-z . .