Приказ основних података о документу

dc.creatorDojnov, Biljana
dc.creatorBožić, Nataša
dc.creatorNenadović, V.
dc.creatorIvanović, J.
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:18:41Z
dc.date.available2019-01-30T17:18:41Z
dc.date.issued2008
dc.identifier.issn1096-4959
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/459
dc.description.abstractUsing soluble starch as a substrate five isoforms of α-amylase were identified in a crude extract of Morimus funereus larvae. The main α-amylase (termed AMF-3) was purified by gel filtration chromatography and anion exchange chromatography to obtain a single band on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Its enzymatic purity was confirmed by an in-gel activity assay after SDS-PAGE. The purity of AMF-3 was increased 112-fold with a 15.4% yield. AMF-3 had apparent molecular masses of 33 and 31 kDa when analysed using SDS-PAGE and Superdex 75 FPLC gel filtration chromatography, respectively and a calculated isoelectric point of 3.2. Purified AMF-3 showed maximal activity at pH 5.2 and had an optimum activity temperature of 45 °C. AMF-3 retained over 90% of its maximum activity at temperatures from 45 to 60 °C. AMF-3 exhibited a high affinity towards soluble starch with a Km value of 0.43 mg/mL. Maximal AMF-3 activity was achieved in the presence of 0.1 mM CaCl2, while at higher concentrations its activity decreased. AMF-3 activity increased with increasing NaCl concentration. AMF-3 activity was significantly inhibited by α-amylase wheat inhibitor. Using a number of raw starch substrates maximum AMF-3 activity was achieved with horse-radish starch, in contrast to undetectable activity towards potato starch.en
dc.publisherElsevier Science Inc, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsrestrictedAccess
dc.sourceComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
dc.subjectCerambycid beetleen
dc.subjectIsoformen
dc.subjectMidgut a-amylaseen
dc.subjectMorimus funereusen
dc.subjectXylophagous larvaeen
dc.titlePurification and properties of midgut α-amylase isolated from Morimus funereus (Coleoptera: Cerambycidae) larvaeen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractНенадовић, В.; Божић, Наташа; Дојнов, Биљана; Ивановић, Ј.; Вујчић, З.;
dc.citation.volume149
dc.citation.issue1
dc.citation.spage153
dc.citation.epage160
dc.citation.other149(1): 153-160
dc.citation.rankM22
dc.identifier.pmid17942357
dc.identifier.doi10.1016/j.cbpb.2007.09.009
dc.identifier.scopus2-s2.0-36849076255
dc.identifier.wos000254219600016
dc.type.versionpublishedVersion


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Приказ основних података о документу