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dc.creatorPopović, Mina
dc.creatorStojanović, Marijana
dc.creatorVeličković, Zlate
dc.creatorKovačević, Ana
dc.creatorMiljković, Radmila
dc.creatorMirković, Nemanja
dc.creatorMarinković, Aleksandar D.
dc.date.accessioned2021-05-11T13:27:28Z
dc.date.available2021-05-11T13:27:28Z
dc.date.issued2021
dc.identifier.issn0141-8130
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/4594
dc.description.abstractIn this study, Lactobacillus reuteri B2was isolated fromthe feces of C57BL/6 mice and assessed on probiotic activity. L. reuteri B2was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0was 64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2 showed maximumdiameters against Klebsiela oxytoca J7 (12.5±0.71mm).Wefurther hypothesized if L. reuteri B2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of the appropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulated into sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterization materials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy (SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at lowpH from2.0 to 4.0 and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobial activity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices (SGJ).sr
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200177/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS//sr
dc.relation.isversionofhttps://cer.ihtm.bg.ac.rs/handle/123456789/4595
dc.relation.isreferencedbyhttps://cer.ihtm.bg.ac.rs/handle/123456789/4596
dc.rightsrestrictedAccesssr
dc.sourceInternational Journal of Biological Macromoleculessr
dc.subjectProbioticssr
dc.subjectLactobacills reuterisr
dc.subjectAlginatesr
dc.subjectStarch maleatesr
dc.subjectMicroencapsulationsr
dc.titleCharacterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymerssr
dc.typearticlesr
dc.rights.licenseARRsr
dcterms.abstractВеличковић, Злате; Маринковић, Aлександар; Поповић, Мина; Ковачевић, Aна; Мирковић, Немања; Стојановић, Маријана; Миљковић, Радмила;
dc.citation.volume183
dc.citation.spage423
dc.citation.epage434
dc.citation.rankaM21
dc.description.otherThe peer-reviewed version: [https://cer.ihtm.bg.ac.rs/handle/123456789/4595]
dc.description.otherSupplementary material: [https://cer.ihtm.bg.ac.rs/handle/123456789/4596]
dc.identifier.pmid33932415
dc.identifier.doi10.1016/j.ijbiomac.2021.04.177
dc.identifier.scopus2-s2.0-85105110042
dc.identifier.wos000675542000011
dc.type.versionpublishedVersionsr


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