Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers
Само за регистроване кориснике
2021
Аутори
Popović, MinaStojanović, Marijana
Veličković, Zlate
Kovačević, Ana
Miljković, Radmila
Mirković, Nemanja
Marinković, Aleksandar D.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study, Lactobacillus reuteri B2was isolated fromthe feces of C57BL/6 mice and assessed on probiotic activity.
L. reuteri B2was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0was
64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2
showed maximumdiameters against Klebsiela oxytoca J7 (12.5±0.71mm).Wefurther hypothesized if L. reuteri
B2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of the
appropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulated
into sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterization
materials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy
(SEM), FTIR, and particle size distribution. The high survival rate of L. re...uteri B2 at lowpH from2.0 to 4.0
and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobial
activity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices
(SGJ).
Кључне речи:
Probiotics / Lactobacills reuteri / Alginate / Starch maleate / MicroencapsulationИзвор:
International Journal of Biological Macromolecules, 2021, 183, 423-434Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200177 (Центар за имунолошка истраживања 'Бранислав Јанковић' Торлак, Београд) (RS-MESTD-inst-2020-200177)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
Напомена:
- The peer-reviewed version: https://cer.ihtm.bg.ac.rs/handle/123456789/4595
- Supplementary material: https://cer.ihtm.bg.ac.rs/handle/123456789/4596
Повезане информације:
- Друга верзија
https://cer.ihtm.bg.ac.rs/handle/123456789/4595 - Повезани садржај
https://cer.ihtm.bg.ac.rs/handle/123456789/4596
DOI: 10.1016/j.ijbiomac.2021.04.177
ISSN: 0141-8130
PubMed: 33932415
WoS: 000675542000011
Scopus: 2-s2.0-85105110042
Институција/група
IHTMTY - JOUR AU - Popović, Mina AU - Stojanović, Marijana AU - Veličković, Zlate AU - Kovačević, Ana AU - Miljković, Radmila AU - Mirković, Nemanja AU - Marinković, Aleksandar D. PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4594 AB - In this study, Lactobacillus reuteri B2was isolated fromthe feces of C57BL/6 mice and assessed on probiotic activity. L. reuteri B2was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0was 64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2 showed maximumdiameters against Klebsiela oxytoca J7 (12.5±0.71mm).Wefurther hypothesized if L. reuteri B2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of the appropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulated into sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterization materials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy (SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at lowpH from2.0 to 4.0 and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobial activity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices (SGJ). PB - Elsevier T2 - International Journal of Biological Macromolecules T1 - Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers VL - 183 SP - 423 EP - 434 DO - 10.1016/j.ijbiomac.2021.04.177 ER -
@article{ author = "Popović, Mina and Stojanović, Marijana and Veličković, Zlate and Kovačević, Ana and Miljković, Radmila and Mirković, Nemanja and Marinković, Aleksandar D.", year = "2021", abstract = "In this study, Lactobacillus reuteri B2was isolated fromthe feces of C57BL/6 mice and assessed on probiotic activity. L. reuteri B2was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0was 64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2 showed maximumdiameters against Klebsiela oxytoca J7 (12.5±0.71mm).Wefurther hypothesized if L. reuteri B2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of the appropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulated into sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterization materials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy (SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at lowpH from2.0 to 4.0 and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobial activity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices (SGJ).", publisher = "Elsevier", journal = "International Journal of Biological Macromolecules", title = "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers", volume = "183", pages = "423-434", doi = "10.1016/j.ijbiomac.2021.04.177" }
Popović, M., Stojanović, M., Veličković, Z., Kovačević, A., Miljković, R., Mirković, N.,& Marinković, A. D.. (2021). Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. in International Journal of Biological Macromolecules Elsevier., 183, 423-434. https://doi.org/10.1016/j.ijbiomac.2021.04.177
Popović M, Stojanović M, Veličković Z, Kovačević A, Miljković R, Mirković N, Marinković AD. Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. in International Journal of Biological Macromolecules. 2021;183:423-434. doi:10.1016/j.ijbiomac.2021.04.177 .
Popović, Mina, Stojanović, Marijana, Veličković, Zlate, Kovačević, Ana, Miljković, Radmila, Mirković, Nemanja, Marinković, Aleksandar D., "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers" in International Journal of Biological Macromolecules, 183 (2021):423-434, https://doi.org/10.1016/j.ijbiomac.2021.04.177 . .