Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography
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2021
Authors
Lugonja, Nikoleta
Gorjanović, Stanislava Ž.

Pastor, Ferenc T.

Marinković, Vesna
Miličić, Biljana
Vrvić, Miroslav

Spasić, Snežana

Article (Published version)

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Antioxidants present in infant food contribute to its nutritive value and provide protection from oxidative damage. Here, a direct current polarographic method, based on a decrease of anodic current from the hydroxoperhydroxo-mercury(II) complex (HPMC) formed at a mercury dropping electrode at its potential of dissolution, was used to determine the total antioxidant capacity (TAC) of infant formulas, human milk collected from mothers of full-term and preterm infants, human milk supplemented with fortifier, and cow milk. TAC of milk samples and infant formula correlated with protein content measured by the Bradford method (Pearson correlation coefficient 0.605, p<0.001), while TAC of human milk correlated with content of free thiol groups measured by the Ellman method (0.779, p<0.001). The results obtained showed that, in contrast to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, HPMC enables specific insight into the quality of infant milk food which reflects the amounts of free thiol... groups and protein content. The significant contribution of milk proteins and the most prevalent amino acids, particularly cysteine, arginine, and phenylalanine, to TAC was shown. Thus, the HPMC method might be useful in monitoring the quality of infant formulas and human milk for preterm infants before and after supplementation with fortifier.
Keywords:
AntioxidantInfant / Infant Formula / Milk / Polarography / Proteins / Thiols / Amino acids / Antioxidants / Correlation methods / Mercury compounds / Polarographs / Proteins / 2 ,2-diphenyl-1-picrylhydrazyl / Antioxidant capacity / Direct current polarographies / Free thiol group / Oxidative damage / Pearson correlation coefficients / Polarographic method / Total antioxidant capacities (TAC) / DairiesSource:
Food Analytical Methods, 2021Publisher:
- Springer
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-200026)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200051 (Institute of General and Physical Chemistry, Belgrade) (RS-200051)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200168 (University of Belgrade, Faculty of Chemistry) (RS-200168)
DOI: 10.1007/s12161-021-02030-3
ISSN: 1936-9751; 1936-976X
WoS: 000640762100001
Scopus: 2-s2.0-85104386895
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IHTMTY - JOUR AU - Lugonja, Nikoleta AU - Gorjanović, Stanislava Ž. AU - Pastor, Ferenc T. AU - Marinković, Vesna AU - Miličić, Biljana AU - Vrvić, Miroslav AU - Spasić, Snežana PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4574 AB - Antioxidants present in infant food contribute to its nutritive value and provide protection from oxidative damage. Here, a direct current polarographic method, based on a decrease of anodic current from the hydroxoperhydroxo-mercury(II) complex (HPMC) formed at a mercury dropping electrode at its potential of dissolution, was used to determine the total antioxidant capacity (TAC) of infant formulas, human milk collected from mothers of full-term and preterm infants, human milk supplemented with fortifier, and cow milk. TAC of milk samples and infant formula correlated with protein content measured by the Bradford method (Pearson correlation coefficient 0.605, p<0.001), while TAC of human milk correlated with content of free thiol groups measured by the Ellman method (0.779, p<0.001). The results obtained showed that, in contrast to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, HPMC enables specific insight into the quality of infant milk food which reflects the amounts of free thiol groups and protein content. The significant contribution of milk proteins and the most prevalent amino acids, particularly cysteine, arginine, and phenylalanine, to TAC was shown. Thus, the HPMC method might be useful in monitoring the quality of infant formulas and human milk for preterm infants before and after supplementation with fortifier. PB - Springer T2 - Food Analytical Methods T1 - Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography DO - 10.1007/s12161-021-02030-3 ER -
@article{ author = "Lugonja, Nikoleta and Gorjanović, Stanislava Ž. and Pastor, Ferenc T. and Marinković, Vesna and Miličić, Biljana and Vrvić, Miroslav and Spasić, Snežana", year = "2021", abstract = "Antioxidants present in infant food contribute to its nutritive value and provide protection from oxidative damage. Here, a direct current polarographic method, based on a decrease of anodic current from the hydroxoperhydroxo-mercury(II) complex (HPMC) formed at a mercury dropping electrode at its potential of dissolution, was used to determine the total antioxidant capacity (TAC) of infant formulas, human milk collected from mothers of full-term and preterm infants, human milk supplemented with fortifier, and cow milk. TAC of milk samples and infant formula correlated with protein content measured by the Bradford method (Pearson correlation coefficient 0.605, p<0.001), while TAC of human milk correlated with content of free thiol groups measured by the Ellman method (0.779, p<0.001). The results obtained showed that, in contrast to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, HPMC enables specific insight into the quality of infant milk food which reflects the amounts of free thiol groups and protein content. The significant contribution of milk proteins and the most prevalent amino acids, particularly cysteine, arginine, and phenylalanine, to TAC was shown. Thus, the HPMC method might be useful in monitoring the quality of infant formulas and human milk for preterm infants before and after supplementation with fortifier.", publisher = "Springer", journal = "Food Analytical Methods", title = "Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography", doi = "10.1007/s12161-021-02030-3" }
Lugonja, N., Gorjanović, S. Ž., Pastor, F. T., Marinković, V., Miličić, B., Vrvić, M.,& Spasić, S.. (2021). Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography. in Food Analytical Methods Springer.. https://doi.org/10.1007/s12161-021-02030-3
Lugonja N, Gorjanović SŽ, Pastor FT, Marinković V, Miličić B, Vrvić M, Spasić S. Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography. in Food Analytical Methods. 2021;. doi:10.1007/s12161-021-02030-3 .
Lugonja, Nikoleta, Gorjanović, Stanislava Ž., Pastor, Ferenc T., Marinković, Vesna, Miličić, Biljana, Vrvić, Miroslav, Spasić, Snežana, "Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography" in Food Analytical Methods (2021), https://doi.org/10.1007/s12161-021-02030-3 . .
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