Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release
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2021
Authors
Jauković, ValentinaKrajišnik, Danina R.
Daković, Aleksandra S.
Damjanović, Ana B.
Krstić, Jugoslav

Stojanović, Jovica
Čalija, Bojan

Article (Published version)

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The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XR...PD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.
Keywords:
Chitosan / drug / Polymer binding / Halloysite nanotubes / Chitosan / Nanocomposites / Etching / Aceclofenac / Sustained releaseSource:
Materials Science and Engineering C, 2021, 123, 112029-Publisher:
- Elsevier
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200161 (University of Belgrade, Faculty of Pharmacy) (RS-200161)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200043 (Institute of Oncology and Radiology of Serbia, Belgrade) (RS-200043)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200023 (Institute of Technology of Nuclear and Other Mineral Row Materials - ITNMS, Belgrade) (RS-200023)
DOI: 10.1016/j.msec.2021.112029
ISSN: 0928-4931; 1873-0191
WoS: 000636846100003
Scopus: 2-s2.0-85102582860
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IHTMTY - JOUR AU - Jauković, Valentina AU - Krajišnik, Danina R. AU - Daković, Aleksandra S. AU - Damjanović, Ana B. AU - Krstić, Jugoslav AU - Stojanović, Jovica AU - Čalija, Bojan PY - 2021 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4487 AB - The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility. PB - Elsevier T2 - Materials Science and Engineering C T1 - Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release VL - 123 SP - 112029 DO - 10.1016/j.msec.2021.112029 ER -
@article{ author = "Jauković, Valentina and Krajišnik, Danina R. and Daković, Aleksandra S. and Damjanović, Ana B. and Krstić, Jugoslav and Stojanović, Jovica and Čalija, Bojan", year = "2021", abstract = "The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.", publisher = "Elsevier", journal = "Materials Science and Engineering C", title = "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release", volume = "123", pages = "112029", doi = "10.1016/j.msec.2021.112029" }
Jauković, V., Krajišnik, D. R., Daković, A. S., Damjanović, A. B., Krstić, J., Stojanović, J.,& Čalija, B.. (2021). Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C Elsevier., 123, 112029. https://doi.org/10.1016/j.msec.2021.112029
Jauković V, Krajišnik DR, Daković AS, Damjanović AB, Krstić J, Stojanović J, Čalija B. Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C. 2021;123:112029. doi:10.1016/j.msec.2021.112029 .
Jauković, Valentina, Krajišnik, Danina R., Daković, Aleksandra S., Damjanović, Ana B., Krstić, Jugoslav, Stojanović, Jovica, Čalija, Bojan, "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release" in Materials Science and Engineering C, 123 (2021):112029, https://doi.org/10.1016/j.msec.2021.112029 . .