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dc.creatorDrača, Dijana
dc.creatorEdeler, David
dc.creatorSaoud, Mohamad
dc.creatorDojčinović, Biljana
dc.creatorDunđerović, Duško
dc.creatorĐmura, Goran
dc.creatorMaksimović-Ivanić, Danijela D.
dc.creatorMijatović, Sanja A.
dc.creatorKaluđerović, Goran N.
dc.date.accessioned2021-04-21T07:34:32Z
dc.date.available2021-04-21T07:34:32Z
dc.date.issued2021
dc.identifier.issn0162-0134
dc.identifier.issn1873-3344
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/4481
dc.description.abstractCP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4′,6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2′,7′-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP.sr
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS//sr
dc.relation.isreferencedbyhttps://cer.ihtm.bg.ac.rs/handle/123456789/4482
dc.rightsrestrictedAccesssr
dc.sourceJournal of Inorganic Biochemistrysr
dc.subjectApoptosissr
dc.subjectAutophagysr
dc.subjectCisplatinsr
dc.subjectCytotoxicitysr
dc.subjectDrug carriersr
dc.subjectSBA-15sr
dc.titleAntitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studiessr
dc.typearticlesr
dc.rights.licenseARRsr
dc.rights.holderElseviersr
dc.citation.volume217
dc.citation.spage111383
dc.citation.rankM21~
dc.description.otherSupplementary material: [https://cer.ihtm.bg.ac.rs/handle/123456789/4482]
dc.identifier.pmid33582397
dc.identifier.doi10.1016/j.jinorgbio.2021.111383
dc.identifier.scopus2-s2.0-85100813341
dc.type.versionpublishedVersionsr


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