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Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies
dc.creator | Drača, Dijana | |
dc.creator | Edeler, David | |
dc.creator | Saoud, Mohamad | |
dc.creator | Dojčinović, Biljana | |
dc.creator | Dunđerović, Duško | |
dc.creator | Đmura, Goran | |
dc.creator | Maksimović-Ivanić, Danijela D. | |
dc.creator | Mijatović, Sanja A. | |
dc.creator | Kaluđerović, Goran N. | |
dc.date.accessioned | 2021-04-21T07:34:32Z | |
dc.date.available | 2021-04-21T07:34:32Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0162-0134 | |
dc.identifier.issn | 1873-3344 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/4481 | |
dc.description.abstract | CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4′,6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2′,7′-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200007/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS// | sr |
dc.relation.isreferencedby | https://cer.ihtm.bg.ac.rs/handle/123456789/4482 | |
dc.rights | restrictedAccess | sr |
dc.source | Journal of Inorganic Biochemistry | sr |
dc.subject | Apoptosis | sr |
dc.subject | Autophagy | sr |
dc.subject | Cisplatin | sr |
dc.subject | Cytotoxicity | sr |
dc.subject | Drug carrier | sr |
dc.subject | SBA-15 | sr |
dc.title | Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | Elsevier | sr |
dc.citation.volume | 217 | |
dc.citation.spage | 111383 | |
dc.citation.rank | M21~ | |
dc.description.other | Supplementary material: [https://cer.ihtm.bg.ac.rs/handle/123456789/4482] | |
dc.identifier.pmid | 33582397 | |
dc.identifier.doi | 10.1016/j.jinorgbio.2021.111383 | |
dc.identifier.scopus | 2-s2.0-85100813341 | |
dc.identifier.wos | 000632487200001 | |
dc.type.version | publishedVersion | sr |