Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae
Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)
dc.creator | Božić, Nataša | |
dc.creator | Dojnov, Biljana | |
dc.creator | Milovanović, Aleksandra | |
dc.creator | Nenadović, Vera | |
dc.creator | Ivanović, Jelisaveta | |
dc.creator | Vujčić, Zoran | |
dc.date.accessioned | 2019-01-30T17:18:07Z | |
dc.date.available | 2019-01-30T17:18:07Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 0354-4664 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/432 | |
dc.description.abstract | Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities. | en |
dc.description.abstract | Primenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni. | sr |
dc.publisher | University of Belgrade, University of Novi Sad | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Archives of Biological Sciences | |
dc.subject | Cerambycid beetle | en |
dc.subject | Morimus funereus | en |
dc.subject | midgut peptidases | en |
dc.subject | protease inhibitors | en |
dc.subject | synthetic substrate | en |
dc.subject | zymogram | en |
dc.title | Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae | en |
dc.title | Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae) | sr |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Дојнов, Биљана; Божић, Наташа; Ненадовић, Вера; Вујчић, З.; Ивановић, Јелисавета; Милутиновић Николић, Aлександра; | |
dc.citation.volume | 60 | |
dc.citation.issue | 3 | |
dc.citation.spage | 403 | |
dc.citation.epage | 409 | |
dc.citation.other | 60(3): 403-409 | |
dc.identifier.doi | 10.2298/ABS0803403B | |
dc.identifier.fulltext | https://cer.ihtm.bg.ac.rs//bitstream/id/9084/430.pdf | |
dc.identifier.scopus | 2-s2.0-53549104726 | |
dc.identifier.wos | 000258524700008 | |
dc.type.version | publishedVersion |