Show simple item record

Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)

dc.creatorBožić, Nataša
dc.creatorDojnov, Biljana
dc.creatorMilovanović, Aleksandra
dc.creatorNenadović, Vera
dc.creatorIvanović, Jelisaveta
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:18:07Z
dc.date.available2019-01-30T17:18:07Z
dc.date.issued2008
dc.identifier.issn0354-4664
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/432
dc.description.abstractApplication of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities.en
dc.description.abstractPrimenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni.sr
dc.publisherUniversity of Belgrade, University of Novi Sad
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsopenAccess
dc.sourceArchives of Biological Sciences
dc.subjectCerambycid beetleen
dc.subjectMorimus funereusen
dc.subjectmidgut peptidasesen
dc.subjectprotease inhibitorsen
dc.subjectsynthetic substrateen
dc.subjectzymogramen
dc.titleCharacterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvaeen
dc.titleKarakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)sr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractДојнов, Биљана; Божић, Наташа; Ненадовић, Вера; Вујчић, З.; Ивановић, Јелисавета; Милутиновић Николић, Aлександра;
dc.citation.volume60
dc.citation.issue3
dc.citation.spage403
dc.citation.epage409
dc.citation.other60(3): 403-409
dc.identifier.doi10.2298/ABS0803403B
dc.identifier.rcubConv_353
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/9084/430.pdf
dc.identifier.scopus2-s2.0-53549104726
dc.identifier.wos000258524700008
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record