CER - Central Repository
Institute of Chemistry, Technology and Metallurgy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   CER
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
  •   CER
  • IHTM
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae

Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)

Thumbnail
2008
430.pdf (216.7Kb)
Authors
Božić, Nataša
Dojnov, Biljana
Milovanović, Aleksandra
Nenadović, Vera
Ivanović, Jelisaveta
Vujčić, Zoran
Article (Published version)
Metadata
Show full item record
Abstract
Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities.
Primenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni.
Keywords:
Cerambycid beetle / Morimus funereus / midgut peptidases / protease inhibitors / synthetic substrate / zymogram
Source:
Archives of Biological Sciences, 2008, 60, 3, 403-409
Publisher:
  • University of Belgrade, University of Novi Sad
Funding / projects:
  • Interakcije prirodnih proizvoda i njihovih analoga sa proteinima i nukleinskim kiselinama (RS-142026)

DOI: 10.2298/ABS0803403B

ISSN: 0354-4664

WoS: 000258524700008

Scopus: 2-s2.0-53549104726
[ Google Scholar ]
2
2
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/432
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Božić, Nataša
AU  - Dojnov, Biljana
AU  - Milovanović, Aleksandra
AU  - Nenadović, Vera
AU  - Ivanović, Jelisaveta
AU  - Vujčić, Zoran
PY  - 2008
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/432
AB  - Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities.
AB  - Primenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni.
PB  - University of Belgrade, University of Novi Sad
T2  - Archives of Biological Sciences
T1  - Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae
T1  - Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)
VL  - 60
IS  - 3
SP  - 403
EP  - 409
DO  - 10.2298/ABS0803403B
UR  - Conv_353
ER  - 
@article{
author = "Božić, Nataša and Dojnov, Biljana and Milovanović, Aleksandra and Nenadović, Vera and Ivanović, Jelisaveta and Vujčić, Zoran",
year = "2008",
abstract = "Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities., Primenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae, Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)",
volume = "60",
number = "3",
pages = "403-409",
doi = "10.2298/ABS0803403B",
url = "Conv_353"
}
Božić, N., Dojnov, B., Milovanović, A., Nenadović, V., Ivanović, J.,& Vujčić, Z.. (2008). Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 60(3), 403-409.
https://doi.org/10.2298/ABS0803403B
Conv_353
Božić N, Dojnov B, Milovanović A, Nenadović V, Ivanović J, Vujčić Z. Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae. in Archives of Biological Sciences. 2008;60(3):403-409.
doi:10.2298/ABS0803403B
Conv_353 .
Božić, Nataša, Dojnov, Biljana, Milovanović, Aleksandra, Nenadović, Vera, Ivanović, Jelisaveta, Vujčić, Zoran, "Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae" in Archives of Biological Sciences, 60, no. 3 (2008):403-409,
https://doi.org/10.2298/ABS0803403B .,
Conv_353 .

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

re3dataOpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About CeR – Central Repository | Send Feedback

re3dataOpenAIRERCUB