Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae
Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)

2008
Authors
Božić, Nataša
Dojnov, Biljana

Milovanović, Aleksandra

Nenadović, Vera
Ivanović, Jelisaveta
Vujčić, Zoran

Article (Published version)
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Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities.
Primenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni.
Keywords:
Cerambycid beetle / Morimus funereus / midgut peptidases / protease inhibitors / synthetic substrate / zymogramSource:
Archives of Biological Sciences, 2008, 60, 3, 403-409Publisher:
- University of Belgrade, University of Novi Sad
Funding / projects:
- Interakcije prirodnih proizvoda i njihovih analoga sa proteinima i nukleinskim kiselinama (RS-142026)
DOI: 10.2298/ABS0803403B
ISSN: 0354-4664
WoS: 000258524700008
Scopus: 2-s2.0-53549104726
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IHTMTY - JOUR AU - Božić, Nataša AU - Dojnov, Biljana AU - Milovanović, Aleksandra AU - Nenadović, Vera AU - Ivanović, Jelisaveta AU - Vujčić, Zoran PY - 2008 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/432 AB - Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities. AB - Primenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni. PB - University of Belgrade, University of Novi Sad T2 - Archives of Biological Sciences T1 - Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae T1 - Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae) VL - 60 IS - 3 SP - 403 EP - 409 DO - 10.2298/ABS0803403B ER -
@article{ author = "Božić, Nataša and Dojnov, Biljana and Milovanović, Aleksandra and Nenadović, Vera and Ivanović, Jelisaveta and Vujčić, Zoran", year = "2008", abstract = "Application of specific chromogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenanthroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsin-like activities., Primenom specifičnih hromogenih supstrata, klasno-specifičnih inhibitora i zimogramske analize identifikovano je nekoliko klasa peptidaza u sirovom ekstraktu srednjeg creva larvi koleoptere Morimus funereus. Zimogramskom analizom sa želatinom kao supstratom i fenilmetilsulfonil-fluoridom kao inhibitorom utvrđeno je da su serin-peptidaze najzastupljenije peptidaze u ekstraktu srednjeg creva larvi M. funereus. Zimogramskom analizom sa želatinom kao supstratom i 1,10-fenantrolinom kaoinhibitorom takođe su detektovane metalopeptidaze. Analizom serin-peptidaza, upotrebom specifičnih hromogenih supstrata, dokazano je da su dominantni elastazi-slični enzimi u sirovom ekstraktu srednjeg creva larvi M. funereus, dok su himotripsinima- i tripsinima-slični enzimi manje zastupljeni.", publisher = "University of Belgrade, University of Novi Sad", journal = "Archives of Biological Sciences", title = "Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae, Karakterizacija endopeptidaza srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)", volume = "60", number = "3", pages = "403-409", doi = "10.2298/ABS0803403B" }
Božić, N., Dojnov, B., Milovanović, A., Nenadović, V., Ivanović, J.,& Vujčić, Z.. (2008). Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae. in Archives of Biological Sciences University of Belgrade, University of Novi Sad., 60(3), 403-409. https://doi.org/10.2298/ABS0803403B
Božić N, Dojnov B, Milovanović A, Nenadović V, Ivanović J, Vujčić Z. Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae. in Archives of Biological Sciences. 2008;60(3):403-409. doi:10.2298/ABS0803403B .
Božić, Nataša, Dojnov, Biljana, Milovanović, Aleksandra, Nenadović, Vera, Ivanović, Jelisaveta, Vujčić, Zoran, "Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae" in Archives of Biological Sciences, 60, no. 3 (2008):403-409, https://doi.org/10.2298/ABS0803403B . .