Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma
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2007
Authors
Nikolić, Milan R.
Stanić-Vučinić, Dragana

Baričević, Ivona I.
Jones, David R.B.
Nedić, Olgica

Niketić, Vesna
Article (Published version)

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Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and cor...related with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo.
Keywords:
Cholesterol / HDL cholesterol / Haemoglobin / Lipoproteins / Phospholipids / Red blood cellsSource:
Clinical Biochemistry, 2007, 40, 5-6, 305-309Publisher:
- Elsevier
Funding / projects:
DOI: 10.1016/j.clinbiochem.2006.11.005
ISSN: 0009-9120
PubMed: 17291471
WoS: 000244997400004
Scopus: 2-s2.0-33847313315
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IHTMTY - JOUR AU - Nikolić, Milan R. AU - Stanić-Vučinić, Dragana AU - Baričević, Ivona I. AU - Jones, David R.B. AU - Nedić, Olgica AU - Niketić, Vesna PY - 2007 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4247 AB - Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and correlated with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo. PB - Elsevier T2 - Clinical Biochemistry T1 - Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma VL - 40 IS - 5-6 SP - 305 EP - 309 DO - 10.1016/j.clinbiochem.2006.11.005 ER -
@article{ author = "Nikolić, Milan R. and Stanić-Vučinić, Dragana and Baričević, Ivona I. and Jones, David R.B. and Nedić, Olgica and Niketić, Vesna", year = "2007", abstract = "Objective: The interior of red blood cells (RBCs) contains a variable amount of cholesterol and phospholipids bound to haemoglobin (Hb). This current study was devised to determine if this pool of lipids (termed Hb-Ch) was available for exchange with plasma lipoproteins. Design and methods: We studied the in vitro efflux of lipids from human RBCs into fasting plasma in men with either low (control group) or high Hb-Ch (study group). Results: When plasma was incubated with a two-fold excess of autologous RBCs the plasma cholesterol level increased due to a decrease in the level of cholesterol from the RBC membrane (in the control group) and due to a decrease in the level of cholesterol both from the RBC membrane and the Hb-Ch fraction (in the study group). The loss of Hb-Ch-derived phospholipids during lipid efflux was roughly equal to that of Hb-Ch-derived cholesterol. The loss of RBC cholesterol into plasma high-density lipoproteins (HDL) was more pronounced in our study group and correlated with the loss of cholesterol from Hb-Ch. Conclusion: The Hb-Ch adduct significantly contributes to the lipid efflux from RBCs into plasma. The majority of cholesterol released from Hb-Ch appears in the plasma HDL fraction suggesting that Hb-Ch may play a role in reverse cholesterol transport in vivo.", publisher = "Elsevier", journal = "Clinical Biochemistry", title = "Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma", volume = "40", number = "5-6", pages = "305-309", doi = "10.1016/j.clinbiochem.2006.11.005" }
Nikolić, M. R., Stanić-Vučinić, D., Baričević, I. I., Jones, D. R.B., Nedić, O.,& Niketić, V.. (2007). Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma. in Clinical Biochemistry Elsevier., 40(5-6), 305-309. https://doi.org/10.1016/j.clinbiochem.2006.11.005
Nikolić MR, Stanić-Vučinić D, Baričević II, Jones DR, Nedić O, Niketić V. Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma. in Clinical Biochemistry. 2007;40(5-6):305-309. doi:10.1016/j.clinbiochem.2006.11.005 .
Nikolić, Milan R., Stanić-Vučinić, Dragana, Baričević, Ivona I., Jones, David R.B., Nedić, Olgica, Niketić, Vesna, "Efflux of cholesterol and phospholipids derived from the haemoglobin-lipid adduct in human red blood cells into plasma" in Clinical Biochemistry, 40, no. 5-6 (2007):305-309, https://doi.org/10.1016/j.clinbiochem.2006.11.005 . .