Since the introduction of suitable, highly porous supports and later nonporous and monolithic supports, chromatography has been an indispensibleindispensable method for protein separations on the analytical and preparative scales. The most frequently used methods for protein chromatography are ion exchange, reversed- phase, hydrophobic hydrophobic-interaction chromatography, chromatography on hydroxyapatite and different types of affinity, and pseudo-affinity chromatography. Because of the use of organic solvents during the separation in reversed-phase chromatography, denaturation and loss of biological activity frequently occurs, and this method is less suitable for the separation of biologically active, therapeutic proteins. Chromatography on monolithic supports and chromatography in the displacement mode offer additional opportunities for fast, highly effective separation of proteins on both the analytical and preparative scales.
Keywords:
Liquid chromatography / Protein fractionation / Peptide separations
TY - CHAP
AU - Anđelković, Uroš
AU - Giacometti, Jasminka
AU - Josić, Đuro
PY - 2017
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4233
AB - Since the introduction of suitable, highly porous supports and later nonporous and monolithic supports, chromatography has been an indispensibleindispensable method for protein separations on the analytical and preparative scales. The most frequently used methods for protein chromatography are ion exchange, reversed- phase, hydrophobic hydrophobic-interaction chromatography, chromatography on hydroxyapatite and different types of affinity, and pseudo-affinity chromatography. Because of the use of organic solvents during the separation in reversed-phase chromatography, denaturation and loss of biological activity frequently occurs, and this method is less suitable for the separation of biologically active, therapeutic proteins. Chromatography on monolithic supports and chromatography in the displacement mode offer additional opportunities for fast, highly effective separation of proteins on both the analytical and preparative scales.
PB - Elsevier
T2 - Liquid Chromatography: Applications
T1 - Protein and Peptide Separations
VL - 2
SP - 107
EP - 157
DO - 10.1016/B978-0-12-805392-8.00005-0
ER -
@article{
author = "Anđelković, Uroš and Giacometti, Jasminka and Josić, Đuro",
year = "2017",
url = "https://cer.ihtm.bg.ac.rs/handle/123456789/4233",
abstract = "Since the introduction of suitable, highly porous supports and later nonporous and monolithic supports, chromatography has been an indispensibleindispensable method for protein separations on the analytical and preparative scales. The most frequently used methods for protein chromatography are ion exchange, reversed- phase, hydrophobic hydrophobic-interaction chromatography, chromatography on hydroxyapatite and different types of affinity, and pseudo-affinity chromatography. Because of the use of organic solvents during the separation in reversed-phase chromatography, denaturation and loss of biological activity frequently occurs, and this method is less suitable for the separation of biologically active, therapeutic proteins. Chromatography on monolithic supports and chromatography in the displacement mode offer additional opportunities for fast, highly effective separation of proteins on both the analytical and preparative scales.",
publisher = "Elsevier",
journal = "Liquid Chromatography: Applications",
title = "Protein and Peptide Separations",
volume = "2",
pages = "107-157",
doi = "10.1016/B978-0-12-805392-8.00005-0"
}
Anđelković, U., Giacometti, J.,& Josić, Đ. (2017). Protein and Peptide Separations.
Liquid Chromatography: Applications
Elsevier., 2, 107-157.
https://doi.org/10.1016/B978-0-12-805392-8.00005-0
Anđelković U, Giacometti J, Josić Đ. Protein and Peptide Separations. Liquid Chromatography: Applications. 2017;2:107-157
