Objective: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). Study design: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. Results: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin- lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. Conclusion: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the forma...tion of Hb-Ch. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.
TY - JOUR
AU - Nikolić, Milan
AU - Stanić, Dragana
AU - Antonijevic, Nadežda
AU - Niketić, Vesna
PY - 2004
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4167
AB - Objective: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). Study design: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. Results: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin- lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. Conclusion: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.
PB - Elsevier
T2 - Clinical Biochemistry
T1 - Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?
VL - 37
IS - 1
SP - 22
EP - 26
DO - 10.1016/j.clinbiochem.2003
ER -
@article{
author = "Nikolić, Milan and Stanić, Dragana and Antonijevic, Nadežda and Niketić, Vesna",
year = "2004",
url = "https://cer.ihtm.bg.ac.rs/handle/123456789/4167",
abstract = "Objective: To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated). Study design: Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer. Results: The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin- lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels. Conclusion: To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?",
volume = "37",
number = "1",
pages = "22-26",
doi = "10.1016/j.clinbiochem.2003"
}
Nikolić, M., Stanić, D., Antonijevic, N.,& Niketić, V. (2004). Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?.
Clinical Biochemistry
Elsevier., 37(1), 22-26.
https://doi.org/10.1016/j.clinbiochem.2003
Nikolić M, Stanić D, Antonijevic N, Niketić V. Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?. Clinical Biochemistry. 2004;37(1):22-26
Nikolić Milan, Stanić Dragana, Antonijevic Nadežda, Niketić Vesna, "Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?" Clinical Biochemistry, 37, no. 1 (2004):22-26,
https://doi.org/10.1016/j.clinbiochem.2003 .
