Inactivation of nanocrystalline C60 cytotoxicity by γ-irradiation
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Marković, Zoran M.
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We investigated the effect of γ-irradiation on the cytotoxicity of pure C 60 solubilized in water by using tetrahydrofuran (THF/n-C 60 or THF/n-C 60 ). In contrast to THF/n-C 60 , its γ-irradiated counterpart failed to generate oxygen radicals and cause extracellular signal-regulated kinase (ERK)-dependent necrotic cell death in various types of mammalian cells. Moreover, γ-irradiated THF/n-C 60 protected cells from the oxidative stress induced by native THF/n-C 60 or hydrogen peroxide. The observed biological effects were associated with γ-irradiation-mediated decomposition of THF and subsequent derivatization of the n-C 60 surface. These results for the first time demonstrate γ-irradiation-mediated changes in the physico-chemical properties of THF-prepared nanocrystalline C 60 , resulting in a complete loss of its cytotoxic effect and its conversion to a cytoprotective agent.
Keywords:Carbon / Nanoparticle / Gamma irradiation / Cytotoxicity / Antioxidant
Source:Biomaterials, 2006, 27, 29, 5049-5058