Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ
Samo za registrovane korisnike
2020
Autori
Popović, SlađanaKrizmanić, Jelena
Vidaković, Danijela
Karadžić, Vesna
Milovanović, Željka
Pećić, Marija
Subakov Simić, Gordana
Članak u časopisu (Objavljena verzija)
,
Springer Nature Switzerland
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Domination of certain aerophytic phototrophic group or specific taxon in biofilms is connected with biofilm features recognised in situ. Well-developed, gelatinous, olive to dark-green biofilms are composed mostly of coccoid cyanobacterial forms. The same features, characterised biofilms dominated by one coccoid taxon, except the latter were vividly coloured. Gloeobacter caused the appearance of purple, Gloeocapsa representatives yellow and Chroococcidiopsis black biofilm. The brown to the dark colour of heterocytous biofilms was mainly caused by Nostoc. Simple trichal Cyanobacteria were occasionally present in biofilm, except in one blue-coloured sample. According to the principal component analysis (PCA), well-developed and gelatinous biofilms were correlated with Cyanobacteria, while scanning electron microscopy (SEM) revealed richness of extracellular polymeric substances (EPS) in such biofilms. Biofilm with calcified cyanobacterium (Geitleria cf. calcarea) was also found. Chloroph...yta-abundant biofilms (many rich in Desmococcus), thinner than cyanobacterial, were predominantly green and occasionally yellow and blue. Many were dry when observed in situ (confirmed with PCA), with few being moistened (i.e. Klebsormidium-dominant). Diatom biofilms were usually developed on sediment, mosses or near seeping water (demonstrated by PCA) and were also thinner than cyanobacterial ones. Compared to cyanobacterial biofilms, SEM showed less developed EPS in those rich in diatoms and green algae, where microorganisms are more exposed to the environment. The study demonstrates an easy method for biofilm assessment based on visual characterisation and provides encouragement for more frequent biofilm investigation in caves that can be important from an ecological, biological, biotechnological point of view and which assessment can have an irreplaceable role in potential monitoring and protection.
Ključne reči:
biofilms / coccoid / cyanobacterial forms / Gloeobacter / Gloeocapsa / Chroococcidiopsis / principal component analysis (PCA) / scanning electron microscopy (SEM) / extracellular polymeric substances / monitoringIzvor:
Environmental Monitoring and Assessment, 2020, 192, 11, 720-Izdavač:
- Springer Science and Business Media LLC
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200026 (Univerzitet u Beogradu, Institut za hemiju, tehnologiju i metalurgiju - IHTM) (RS-200026)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-200178)
DOI: 10.1007/s10661-020-08686-4
ISSN: 0167-6369; 1573-2959
WoS: 000585090800001
Scopus: 2-s2.0-85093826698
Institucija/grupa
IHTMTY - JOUR AU - Popović, Slađana AU - Krizmanić, Jelena AU - Vidaković, Danijela AU - Karadžić, Vesna AU - Milovanović, Željka AU - Pećić, Marija AU - Subakov Simić, Gordana PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4025 AB - Domination of certain aerophytic phototrophic group or specific taxon in biofilms is connected with biofilm features recognised in situ. Well-developed, gelatinous, olive to dark-green biofilms are composed mostly of coccoid cyanobacterial forms. The same features, characterised biofilms dominated by one coccoid taxon, except the latter were vividly coloured. Gloeobacter caused the appearance of purple, Gloeocapsa representatives yellow and Chroococcidiopsis black biofilm. The brown to the dark colour of heterocytous biofilms was mainly caused by Nostoc. Simple trichal Cyanobacteria were occasionally present in biofilm, except in one blue-coloured sample. According to the principal component analysis (PCA), well-developed and gelatinous biofilms were correlated with Cyanobacteria, while scanning electron microscopy (SEM) revealed richness of extracellular polymeric substances (EPS) in such biofilms. Biofilm with calcified cyanobacterium (Geitleria cf. calcarea) was also found. Chlorophyta-abundant biofilms (many rich in Desmococcus), thinner than cyanobacterial, were predominantly green and occasionally yellow and blue. Many were dry when observed in situ (confirmed with PCA), with few being moistened (i.e. Klebsormidium-dominant). Diatom biofilms were usually developed on sediment, mosses or near seeping water (demonstrated by PCA) and were also thinner than cyanobacterial ones. Compared to cyanobacterial biofilms, SEM showed less developed EPS in those rich in diatoms and green algae, where microorganisms are more exposed to the environment. The study demonstrates an easy method for biofilm assessment based on visual characterisation and provides encouragement for more frequent biofilm investigation in caves that can be important from an ecological, biological, biotechnological point of view and which assessment can have an irreplaceable role in potential monitoring and protection. PB - Springer Science and Business Media LLC T2 - Environmental Monitoring and Assessment T1 - Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ VL - 192 IS - 11 SP - 720 DO - 10.1007/s10661-020-08686-4 ER -
@article{ author = "Popović, Slađana and Krizmanić, Jelena and Vidaković, Danijela and Karadžić, Vesna and Milovanović, Željka and Pećić, Marija and Subakov Simić, Gordana", year = "2020", abstract = "Domination of certain aerophytic phototrophic group or specific taxon in biofilms is connected with biofilm features recognised in situ. Well-developed, gelatinous, olive to dark-green biofilms are composed mostly of coccoid cyanobacterial forms. The same features, characterised biofilms dominated by one coccoid taxon, except the latter were vividly coloured. Gloeobacter caused the appearance of purple, Gloeocapsa representatives yellow and Chroococcidiopsis black biofilm. The brown to the dark colour of heterocytous biofilms was mainly caused by Nostoc. Simple trichal Cyanobacteria were occasionally present in biofilm, except in one blue-coloured sample. According to the principal component analysis (PCA), well-developed and gelatinous biofilms were correlated with Cyanobacteria, while scanning electron microscopy (SEM) revealed richness of extracellular polymeric substances (EPS) in such biofilms. Biofilm with calcified cyanobacterium (Geitleria cf. calcarea) was also found. Chlorophyta-abundant biofilms (many rich in Desmococcus), thinner than cyanobacterial, were predominantly green and occasionally yellow and blue. Many were dry when observed in situ (confirmed with PCA), with few being moistened (i.e. Klebsormidium-dominant). Diatom biofilms were usually developed on sediment, mosses or near seeping water (demonstrated by PCA) and were also thinner than cyanobacterial ones. Compared to cyanobacterial biofilms, SEM showed less developed EPS in those rich in diatoms and green algae, where microorganisms are more exposed to the environment. The study demonstrates an easy method for biofilm assessment based on visual characterisation and provides encouragement for more frequent biofilm investigation in caves that can be important from an ecological, biological, biotechnological point of view and which assessment can have an irreplaceable role in potential monitoring and protection.", publisher = "Springer Science and Business Media LLC", journal = "Environmental Monitoring and Assessment", title = "Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ", volume = "192", number = "11", pages = "720", doi = "10.1007/s10661-020-08686-4" }
Popović, S., Krizmanić, J., Vidaković, D., Karadžić, V., Milovanović, Ž., Pećić, M.,& Subakov Simić, G.. (2020). Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ. in Environmental Monitoring and Assessment Springer Science and Business Media LLC., 192(11), 720. https://doi.org/10.1007/s10661-020-08686-4
Popović S, Krizmanić J, Vidaković D, Karadžić V, Milovanović Ž, Pećić M, Subakov Simić G. Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ. in Environmental Monitoring and Assessment. 2020;192(11):720. doi:10.1007/s10661-020-08686-4 .
Popović, Slađana, Krizmanić, Jelena, Vidaković, Danijela, Karadžić, Vesna, Milovanović, Željka, Pećić, Marija, Subakov Simić, Gordana, "Biofilms in caves: easy method for the assessment of dominant phototrophic groups/taxa in situ" in Environmental Monitoring and Assessment, 192, no. 11 (2020):720, https://doi.org/10.1007/s10661-020-08686-4 . .