Hydrolytic degradation of star-shaped poly(ε-caprolactone)s with different number of arms and their cytotoxic effects
Authors
Ponjavić, Marijana
Nikolić, Marija S.

Stevanović, Sanja

Nikodinović-Runić, Jasmina

Jeremić, Sanja

Pavić, Aleksandar

Đongalić, Jasna

Article (Published version)
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Star-shaped polymers of biodegradable aliphatic polyester, poly(ε-caprolactone), PCL, with different number of arms (three, four, and six) were synthesized by ring-opening polymerization initiated by multifunctional alcohols used as cores. As potential biomaterials, synthesized star-shaped poly(ε-caprolactone)s, sPCL, were thoroughly characterized in terms of their degradation under different pH conditions and in respect to their cytotoxicity. The in vitro degradation was performed in phosphate buffer (pH 7.4) and hydrochloric acid solution (pH 1.0) over 5 weeks. Degradation of sPCL films was followed by the weight loss measurements, GPC, FTIR, and AFM analysis. While the most of the samples were stable against the abiotic hydrolysis at pH 7.4 after 5 weeks of degradation, degradation was significantly accelerated in the acidic medium. Degradation rate of polymer films was affected by the polymer architecture and molecular weight. The molecular weight profiles during the degradation re...vealed random chain scission of the ester bonds indicating bulk degradation mechanism of hydrolysis at pH 7.4, while acidic hydrolysis proceeded through the bulk degradation associated with surface erosion, confirmed by AFM. The in vitro toxicity tests, cytotoxicity applying normal human fibroblasts (MRC5) and embryotoxicity assessment (using zebra fish model, Danio rerio), suggested those polymeric materials as suitable for biomedical application.
Keywords:
Star-shaped PCLs / hydrolysis at different pH / cytotoxycity / embryotoxycitySource:
Journal of Bioactive and Compatible Polymers, 2020, 35, 6, 517-537Publisher:
- SAGE Publications
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-200135)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-200042)
DOI: 10.1177/0883911520951826
ISSN: 0883-9115; 1530-8030
WoS: 000565911800001
Scopus: 2-s2.0-85090200992
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IHTMTY - JOUR AU - Ponjavić, Marijana AU - Nikolić, Marija S. AU - Stevanović, Sanja AU - Nikodinović-Runić, Jasmina AU - Jeremić, Sanja AU - Pavić, Aleksandar AU - Đongalić, Jasna PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/4019 AB - Star-shaped polymers of biodegradable aliphatic polyester, poly(ε-caprolactone), PCL, with different number of arms (three, four, and six) were synthesized by ring-opening polymerization initiated by multifunctional alcohols used as cores. As potential biomaterials, synthesized star-shaped poly(ε-caprolactone)s, sPCL, were thoroughly characterized in terms of their degradation under different pH conditions and in respect to their cytotoxicity. The in vitro degradation was performed in phosphate buffer (pH 7.4) and hydrochloric acid solution (pH 1.0) over 5 weeks. Degradation of sPCL films was followed by the weight loss measurements, GPC, FTIR, and AFM analysis. While the most of the samples were stable against the abiotic hydrolysis at pH 7.4 after 5 weeks of degradation, degradation was significantly accelerated in the acidic medium. Degradation rate of polymer films was affected by the polymer architecture and molecular weight. The molecular weight profiles during the degradation revealed random chain scission of the ester bonds indicating bulk degradation mechanism of hydrolysis at pH 7.4, while acidic hydrolysis proceeded through the bulk degradation associated with surface erosion, confirmed by AFM. The in vitro toxicity tests, cytotoxicity applying normal human fibroblasts (MRC5) and embryotoxicity assessment (using zebra fish model, Danio rerio), suggested those polymeric materials as suitable for biomedical application. PB - SAGE Publications T2 - Journal of Bioactive and Compatible Polymers T1 - Hydrolytic degradation of star-shaped poly(ε-caprolactone)s with different number of arms and their cytotoxic effects VL - 35 IS - 6 SP - 517 EP - 537 DO - 10.1177/0883911520951826 ER -
@article{ author = "Ponjavić, Marijana and Nikolić, Marija S. and Stevanović, Sanja and Nikodinović-Runić, Jasmina and Jeremić, Sanja and Pavić, Aleksandar and Đongalić, Jasna", year = "2020", abstract = "Star-shaped polymers of biodegradable aliphatic polyester, poly(ε-caprolactone), PCL, with different number of arms (three, four, and six) were synthesized by ring-opening polymerization initiated by multifunctional alcohols used as cores. As potential biomaterials, synthesized star-shaped poly(ε-caprolactone)s, sPCL, were thoroughly characterized in terms of their degradation under different pH conditions and in respect to their cytotoxicity. The in vitro degradation was performed in phosphate buffer (pH 7.4) and hydrochloric acid solution (pH 1.0) over 5 weeks. Degradation of sPCL films was followed by the weight loss measurements, GPC, FTIR, and AFM analysis. While the most of the samples were stable against the abiotic hydrolysis at pH 7.4 after 5 weeks of degradation, degradation was significantly accelerated in the acidic medium. Degradation rate of polymer films was affected by the polymer architecture and molecular weight. The molecular weight profiles during the degradation revealed random chain scission of the ester bonds indicating bulk degradation mechanism of hydrolysis at pH 7.4, while acidic hydrolysis proceeded through the bulk degradation associated with surface erosion, confirmed by AFM. The in vitro toxicity tests, cytotoxicity applying normal human fibroblasts (MRC5) and embryotoxicity assessment (using zebra fish model, Danio rerio), suggested those polymeric materials as suitable for biomedical application.", publisher = "SAGE Publications", journal = "Journal of Bioactive and Compatible Polymers", title = "Hydrolytic degradation of star-shaped poly(ε-caprolactone)s with different number of arms and their cytotoxic effects", volume = "35", number = "6", pages = "517-537", doi = "10.1177/0883911520951826" }
Ponjavić, M., Nikolić, M. S., Stevanović, S., Nikodinović-Runić, J., Jeremić, S., Pavić, A.,& Đongalić, J.. (2020). Hydrolytic degradation of star-shaped poly(ε-caprolactone)s with different number of arms and their cytotoxic effects. in Journal of Bioactive and Compatible Polymers SAGE Publications., 35(6), 517-537. https://doi.org/10.1177/0883911520951826
Ponjavić M, Nikolić MS, Stevanović S, Nikodinović-Runić J, Jeremić S, Pavić A, Đongalić J. Hydrolytic degradation of star-shaped poly(ε-caprolactone)s with different number of arms and their cytotoxic effects. in Journal of Bioactive and Compatible Polymers. 2020;35(6):517-537. doi:10.1177/0883911520951826 .
Ponjavić, Marijana, Nikolić, Marija S., Stevanović, Sanja, Nikodinović-Runić, Jasmina, Jeremić, Sanja, Pavić, Aleksandar, Đongalić, Jasna, "Hydrolytic degradation of star-shaped poly(ε-caprolactone)s with different number of arms and their cytotoxic effects" in Journal of Bioactive and Compatible Polymers, 35, no. 6 (2020):517-537, https://doi.org/10.1177/0883911520951826 . .