Influence of the low frequency 10-1000 Hz magnetic field on Saccharomyces cerevisiae respiration activity
Uticaj niskofrekventnog magnetnog polja (10-1000 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae
Authors
Lješević, MarijaLončarević, Branka
Bubanja, Itana Nuša
Beškoski, Vladimir
Gojgić-Cvijović, Gordana
Velikić, Zoran
Stanisavljev, Dragomir
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The effects of electric, magnetic or electromagnetic fields on different microbes have
become a very popular topic nowadays, mostly because the fields could potentially affect
the survival of the microbial cells as well as their behavior and metabolism. In this paper the
influence of the magnetic field with constant low frequency scan regime from 10 to 1000
Hz on yeast cells S. cerevisiae respiration activity was examined using Micro-Oxymax®
respirometer. Experiments were performed in five replicates. All five experiments showed
lower cumulative O2 consumption in magnetic field exposed samples, compared to the
control sample and inconsistent cumulative CO2 production. The paired two sample onetail
T-test showed statistically significant differences between control and magnetic field
sample for cumulative O2 consumption, but not for the CO2 production. Even though
additional experiments are neccesary to clarify the differencies in CO2 production, these
preliminary findings st...rongly suggest that obtained results represent a good basis for
further investigations in this field.
Veoma popularna tema današnjice je ispitivanje električnog, magnetnog i elektromagnetnog polja na različite mikroorganizme, jer pomenuta fizička polja potencijalno deluju kao faktori stresa i tako utiču na njihovo preživljavanje, ponašanje i metabolizam. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja sa konstantnim intervalom skeniranja od 10 do 1000 Hz na respiraciju ćelija kvasca, Saccharomyces cerevisiae. Eksperimenti su rađeni u pet ponavljanja i kumulativna potrošnja O2 i produkcija CO2 praćena je pomoću Micro-Oxymax® respirometra. U svih pet ponavljanja, ćelije koje su bile izložene magnetnom polju pokazale su manju kumulativnu potrošnju kiseonika u poređenju sa uzorcima van magnetnog polja i nekonzistentnu produkciju CO2. Rezultati su obrađeni uporednim jednosmernim T-testom, koji je pokazao da postoje statistički značajne razlike u kumulativnoj potrošnji O2 između kontrolnih ćelija i onih izloženih magnetnom polju, što nije slučaj sa kumulativnom produkcijom C...O2. Iako su dodatna ispitivanja neophodna da se objasni nekonzistentnost produkcije CO2, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.
Keywords:
magnetic field / yeast cells / O2 consumption / CO2 productionSource:
Proceedings - 55th Meeting of the Serbian Chemical Society, 2018, 31-35Publisher:
- Belgrade : Serbian Chemical Society
URI
https://www.shd.org.rs/index.php/abstracts2018-2/proceedingshttps://cer.ihtm.bg.ac.rs/handle/123456789/3979
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IHTMTY - CONF AU - Lješević, Marija AU - Lončarević, Branka AU - Bubanja, Itana Nuša AU - Beškoski, Vladimir AU - Gojgić-Cvijović, Gordana AU - Velikić, Zoran AU - Stanisavljev, Dragomir PY - 2018 UR - https://www.shd.org.rs/index.php/abstracts2018-2/proceedings UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3979 AB - The effects of electric, magnetic or electromagnetic fields on different microbes have become a very popular topic nowadays, mostly because the fields could potentially affect the survival of the microbial cells as well as their behavior and metabolism. In this paper the influence of the magnetic field with constant low frequency scan regime from 10 to 1000 Hz on yeast cells S. cerevisiae respiration activity was examined using Micro-Oxymax® respirometer. Experiments were performed in five replicates. All five experiments showed lower cumulative O2 consumption in magnetic field exposed samples, compared to the control sample and inconsistent cumulative CO2 production. The paired two sample onetail T-test showed statistically significant differences between control and magnetic field sample for cumulative O2 consumption, but not for the CO2 production. Even though additional experiments are neccesary to clarify the differencies in CO2 production, these preliminary findings strongly suggest that obtained results represent a good basis for further investigations in this field. AB - Veoma popularna tema današnjice je ispitivanje električnog, magnetnog i elektromagnetnog polja na različite mikroorganizme, jer pomenuta fizička polja potencijalno deluju kao faktori stresa i tako utiču na njihovo preživljavanje, ponašanje i metabolizam. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja sa konstantnim intervalom skeniranja od 10 do 1000 Hz na respiraciju ćelija kvasca, Saccharomyces cerevisiae. Eksperimenti su rađeni u pet ponavljanja i kumulativna potrošnja O2 i produkcija CO2 praćena je pomoću Micro-Oxymax® respirometra. U svih pet ponavljanja, ćelije koje su bile izložene magnetnom polju pokazale su manju kumulativnu potrošnju kiseonika u poređenju sa uzorcima van magnetnog polja i nekonzistentnu produkciju CO2. Rezultati su obrađeni uporednim jednosmernim T-testom, koji je pokazao da postoje statistički značajne razlike u kumulativnoj potrošnji O2 između kontrolnih ćelija i onih izloženih magnetnom polju, što nije slučaj sa kumulativnom produkcijom CO2. Iako su dodatna ispitivanja neophodna da se objasni nekonzistentnost produkcije CO2, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti. PB - Belgrade : Serbian Chemical Society C3 - Proceedings - 55th Meeting of the Serbian Chemical Society T1 - Influence of the low frequency 10-1000 Hz magnetic field on Saccharomyces cerevisiae respiration activity T1 - Uticaj niskofrekventnog magnetnog polja (10-1000 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae SP - 31 EP - 35 UR - https://hdl.handle.net/21.15107/rcub_cer_3979 ER -
@conference{ author = "Lješević, Marija and Lončarević, Branka and Bubanja, Itana Nuša and Beškoski, Vladimir and Gojgić-Cvijović, Gordana and Velikić, Zoran and Stanisavljev, Dragomir", year = "2018", abstract = "The effects of electric, magnetic or electromagnetic fields on different microbes have become a very popular topic nowadays, mostly because the fields could potentially affect the survival of the microbial cells as well as their behavior and metabolism. In this paper the influence of the magnetic field with constant low frequency scan regime from 10 to 1000 Hz on yeast cells S. cerevisiae respiration activity was examined using Micro-Oxymax® respirometer. Experiments were performed in five replicates. All five experiments showed lower cumulative O2 consumption in magnetic field exposed samples, compared to the control sample and inconsistent cumulative CO2 production. The paired two sample onetail T-test showed statistically significant differences between control and magnetic field sample for cumulative O2 consumption, but not for the CO2 production. Even though additional experiments are neccesary to clarify the differencies in CO2 production, these preliminary findings strongly suggest that obtained results represent a good basis for further investigations in this field., Veoma popularna tema današnjice je ispitivanje električnog, magnetnog i elektromagnetnog polja na različite mikroorganizme, jer pomenuta fizička polja potencijalno deluju kao faktori stresa i tako utiču na njihovo preživljavanje, ponašanje i metabolizam. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja sa konstantnim intervalom skeniranja od 10 do 1000 Hz na respiraciju ćelija kvasca, Saccharomyces cerevisiae. Eksperimenti su rađeni u pet ponavljanja i kumulativna potrošnja O2 i produkcija CO2 praćena je pomoću Micro-Oxymax® respirometra. U svih pet ponavljanja, ćelije koje su bile izložene magnetnom polju pokazale su manju kumulativnu potrošnju kiseonika u poređenju sa uzorcima van magnetnog polja i nekonzistentnu produkciju CO2. Rezultati su obrađeni uporednim jednosmernim T-testom, koji je pokazao da postoje statistički značajne razlike u kumulativnoj potrošnji O2 između kontrolnih ćelija i onih izloženih magnetnom polju, što nije slučaj sa kumulativnom produkcijom CO2. Iako su dodatna ispitivanja neophodna da se objasni nekonzistentnost produkcije CO2, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.", publisher = "Belgrade : Serbian Chemical Society", journal = "Proceedings - 55th Meeting of the Serbian Chemical Society", title = "Influence of the low frequency 10-1000 Hz magnetic field on Saccharomyces cerevisiae respiration activity, Uticaj niskofrekventnog magnetnog polja (10-1000 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae", pages = "31-35", url = "https://hdl.handle.net/21.15107/rcub_cer_3979" }
Lješević, M., Lončarević, B., Bubanja, I. N., Beškoski, V., Gojgić-Cvijović, G., Velikić, Z.,& Stanisavljev, D.. (2018). Influence of the low frequency 10-1000 Hz magnetic field on Saccharomyces cerevisiae respiration activity. in Proceedings - 55th Meeting of the Serbian Chemical Society Belgrade : Serbian Chemical Society., 31-35. https://hdl.handle.net/21.15107/rcub_cer_3979
Lješević M, Lončarević B, Bubanja IN, Beškoski V, Gojgić-Cvijović G, Velikić Z, Stanisavljev D. Influence of the low frequency 10-1000 Hz magnetic field on Saccharomyces cerevisiae respiration activity. in Proceedings - 55th Meeting of the Serbian Chemical Society. 2018;:31-35. https://hdl.handle.net/21.15107/rcub_cer_3979 .
Lješević, Marija, Lončarević, Branka, Bubanja, Itana Nuša, Beškoski, Vladimir, Gojgić-Cvijović, Gordana, Velikić, Zoran, Stanisavljev, Dragomir, "Influence of the low frequency 10-1000 Hz magnetic field on Saccharomyces cerevisiae respiration activity" in Proceedings - 55th Meeting of the Serbian Chemical Society (2018):31-35, https://hdl.handle.net/21.15107/rcub_cer_3979 .