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dc.creatorĐukanović, Stefana
dc.creatorCvetković, Stefana
dc.creatorLončarević, Branka
dc.creatorLješević, Marija
dc.creatorNikolić, Biljana
dc.creatorSimin, Nataša
dc.creatorBekvalac, Kristina
dc.creatorKekić, Dušan
dc.creatorMitić-Ćulafić, Dragana
dc.date.accessioned2020-12-02T15:48:46Z
dc.date.available2020-12-02T15:48:46Z
dc.date.issued2020
dc.identifier.issn0926-6690
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/3946
dc.description.abstractStaphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.en
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200178/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceIndustrial Crops & Productssr
dc.subjectStaphylococcus aureussr
dc.subjectFrangula alnussr
dc.subjectBiofilmsr
dc.subjectAerobic respirationsr
dc.subjectSEM microscopysr
dc.titleAntistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extracten
dc.typearticlesr
dc.rights.licenseARRsr
dcterms.abstractЛончаревић, Бранка; Кекић, Душан; Ђукановић, Стефана; Цветковић, Стефана; Љешевић, Марија; Николић, Биљана; Симин, Наташа; Беквалац, Кристина; Митић-Ћулафић, Драгана;
dc.rights.holderElseviersr
dc.citation.volume158
dc.citation.spage113013
dc.citation.rankaM21~
dc.identifier.doi10.1016/j.indcrop.2020.113013
dc.identifier.scopus2-s2.0-85092675847
dc.identifier.wos000596374900003
dc.type.versionpublishedVersionsr


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