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dc.creatorSpasojević, Ivan
dc.creatorMojović, Miloš
dc.creatorBlagojević, Duško
dc.creatorSpasić, Snežana
dc.creatorJones, David
dc.creatorNikolić-Kokić, Aleksandra
dc.creatorSpasić, Mihajlo
dc.date.accessioned2020-12-02T11:46:58Z
dc.date.available2020-12-02T11:46:58Z
dc.date.issued2009
dc.identifier.issn0008-6215
dc.identifier.issn1873-426X
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/3937
dc.description.abstractThe hydroxyl radical (radical dotOH) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P); fructose-6-phosphate (F6P); and fructose-1,6-bis(phosphate) (F16BP)) on radical dotOH radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of radical dotOH radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber–Weiss-like system was also employed to generate radical dotOH, so that the effect of iron sequestration could be distinguished from direct radical dotOH radical scavenging. In the latter system, the rank order of radical dotOH scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body’s reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.en
dc.language.isoensr
dc.publisherElseviersr
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143034/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143016/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceCarbohydrate Researchsr
dc.subjectFructosesr
dc.subjectGlucosesr
dc.subjectHydroxyl radicalsr
dc.subjectMannitolsr
dc.subjectEPR spectroscopysr
dc.subjectHaber–Weiss reactionsr
dc.titleRelevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radicalen
dc.typearticlesr
dc.rights.licenseARRsr
dcterms.abstractМојовић, Милош; Спасојевић, Иван; Благојевић, Душко; Спасић, Снежана; Јонес, Давид; Николић-Кокић, Aлександра; Спасић, Михајло;
dc.rights.holderElseviersr
dc.citation.volume344
dc.citation.issue1
dc.citation.spage80
dc.citation.epage84
dc.citation.rankM21
dc.identifier.doi10.1016/j.carres.2008.09.025
dc.identifier.scopus2-s2.0-57749111966
dc.identifier.wos000262596200011
dc.type.versionpublishedVersionsr


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