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Antioxidant properties of grape seed extract on human lymphocyte oxidative defence

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2008
Authors
Stanković, Miroslava
Tešević, Vele
Vajs, Vlatka
Todorović, Nina
Milosavljević, Slobodan
Gođevac, Dejan
Article (Published version)
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Abstract
The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and C-13-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyt...e damage by ROS.

Keywords:
Vitis vinifera / Vitaceae / micronucleus test / antioxidant enzymes / human lymphocytes / LC/MS / NMR
Source:
Planta Medica, 2008, 74, 7, 730-735
Publisher:
  • Georg Thieme Verlag Kg, Stuttgart
Projects:
  • Sekundarni metaboliti samoniklih, lekovitih biljaka: izolovanje, karakterizacija i biloška aktivnost (RS-142053)

DOI: 10.1055/s-2008-1074521

ISSN: 0032-0943

PubMed: 18496781

WoS: 000257284700005

Scopus: 2-s2.0-45849128011
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URI
http://cer.ihtm.bg.ac.rs/handle/123456789/391
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IHTM

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