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SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
dc.creator | Nešić, Maja D. | |
dc.creator | Dučić, Tanja | |
dc.creator | Liang, Xinyue | |
dc.creator | Algarra, Manuel | |
dc.creator | Mi, Lan | |
dc.creator | Korićanac, Lela | |
dc.creator | Žakula, Jelena | |
dc.creator | Kop, Tatjana | |
dc.creator | Bjelaković, Mira | |
dc.creator | Mitrović, Aleksandra | |
dc.creator | Gojgić-Cvijović, Gordana | |
dc.creator | Stepić, Milutin | |
dc.creator | Petković, Marijana | |
dc.date.accessioned | 2020-12-01T06:54:05Z | |
dc.date.available | 2020-12-01T06:54:05Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0141-8130 | |
dc.identifier.issn | 1879-0003 (web) | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/3891 | |
dc.description.abstract | Abstract Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide Iband, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells. | en |
dc.language.iso | en | sr |
dc.publisher | Elsevier | sr |
dc.relation | Agência Regional para o Desenvolvimento da Investigação, Tecnologia e Inovação (M1420-01-0145-FEDER-000005) | sr |
dc.relation | Ministry of Science and Technology of the People's Republic of China (SINO-SERBIA2018002) | sr |
dc.relation | Madeira 14-20 Program (RAM-M1420-01-0145-FEDER-000008) | sr |
dc.relation | Serbian-Chinese bilateral project (451-00-478/2018-09/16) | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200017/RS// | sr |
dc.relation | ALBA Synchrotron (Grant No. 2019093770) | sr |
dc.relation | Fundação para a Ciência e a Tecnologia (PEstOE/QUI/UI0674/2019) | sr |
dc.relation | Fundação para a Ciência e a Tecnologia (INNOINDIGO/0001/2015) | sr |
dc.relation | Fundação para a Ciência e a Tecnologia (UID/MAT/00006/2019) | sr |
dc.relation | Ministerio de Ciencia, Innovación y Universidades (RTI2018-099668-BC22) | sr |
dc.relation | Junta de Andalucía and FEDER funds (UMA18-FEDERJA-126) | sr |
dc.rights | restrictedAccess | sr |
dc.source | International Journal of Biological Macromolecules | sr |
dc.subject | Pullulan-C60 | sr |
dc.subject | Levan-C60 | sr |
dc.subject | Synchrotron radiation FTIR spectro-microscopy | sr |
dc.title | SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives | en |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dcterms.abstract | Нешић, Маја, Д.; Бјелаковић, Мира С.; Дучић, Тања; Aлгарра, Мануел; Коп, Татјана Ј.; Корићанац, Лела; Ми, Лан; Петковић, Маријана; Лианг, Xинyуе; Митровић, Aлександра; Гојгић Цвијовић, Гордана Д.; Степић, Милутин; Жакула, Јелена; | |
dc.rights.holder | Elsevier | sr |
dc.citation.volume | 165 | |
dc.citation.issue | B | |
dc.citation.spage | 2541 | |
dc.citation.epage | 2549 | |
dc.citation.rank | aM21~ | |
dc.identifier.doi | 10.1016/j.ijbiomac.2020.10.141 | |
dc.identifier.scopus | 2-s2.0-85094207129 | |
dc.identifier.wos | 000600773500088 | |
dc.type.version | publishedVersion | sr |