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Determination of serum glycosylated proteins: Comparison of thiobarbituric acid and phenol-sulphuric acid assays

dc.creatorMandić, Ljuba
dc.creatorStojanović, Srđan
dc.creatorČolović, Ana
dc.creatorMilin, Nenad
dc.date.accessioned2020-11-30T11:55:03Z
dc.date.available2020-11-30T11:55:03Z
dc.date.issued2000
dc.identifier.issn0354-3447
dc.identifier.urihttps://cer.ihtm.bg.ac.rs/handle/123456789/3889
dc.description.abstractOdređivanje sadržaja glikozilovanih proteina je važno za otkrivanje i kontrolu stanja dugotrajne hiperglikemijc u dijabetesu. Cl ovom radu određivan je sadržaj glukoze vezane za proteine seruma kod obolelih od dijabetesa, spektrolotometrijskim metodama s fenol-sumpornom kiselinom (PS) i s tiobarbiturnom kiselinom (TBA). Vrednosti vezane glukoze dobijene s PS reagensom (80,6 ± 22,8 gmol glukoze/g proteina) su bile znatno više od do-bijenih s TBA metodom (3,71 ± 1,34 pmol glukoze/g proteina). Analiza faktora, koji bi mogli dovesti do ove značajne razlike, je pokazala da su više vrednosti dobijene PS metodom posledica dodatne dehidratacije ugljenih hidrata oslobođenih u toku hidrolize glikozilovanih proteina. Do dehidratacije dolazi pri izvođenju bojene reakcije s fenol-sumpornom kiselinom. Zaključeno je da, iako se osetljivost obe metode za fruktozu kao standard ne razlikuje značajno, TBA metoda je pouzdanija od PS metode i preporučuje se za kliničku praksu.sr
dc.description.abstractThe estimation of glycosylated proteins is useful in the detection and control of long-standing hyperglycemic conditions. In this paper the content of protein-bound glucose was determined in diabetics by spectrophotometric method with phenol-sulphuric acid (PS) and with thiobarbituric acid (TBA). The amounts of protein-bound glucose obtained with the PS reagent (80.6 ± 22.8 mmol glucose/g protein) were always considerably higher than those obtained by TBA method (3.71 ± 1.34 μmol glucose/g protein). The analysis was made of parameters that might have led to such significant difference. It was shown that in the reaction with PS reagent there was an additional dehydration of carbohydrate released during the hydrolysis of glycosylated proteins. The additional dehydration was due to concentrated sulphuric acid which at higher temperature influenced chromogen formation. On the basis of the results obtained it was concluded that, although the sensitivity of the both methods for standard fructose was comparable, TBA method was more reliable than PS method. Therefore, it is recommended in clinical practice.en
dc.language.isosrsr
dc.publisherDruštvo medicinskih biohemičara Srbije i Crne Gore i Farmaceutski fakultet, Beogradsr
dc.rightsopenAccesssr
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceJugoslovenska medicinska biohemija - Yugoslav Medical Biochemistrysr
dc.subjectGlikozilovani proteinisr
dc.subjectFenol-sumporna metodasr
dc.subjectTiobarbiturna metodasr
dc.subjectGlycosylated proteinssr
dc.subjectPhenol-sulphuric methodsr
dc.subjectThiobarbituric methodsr
dc.titleOdredivanje glikozilovanih proteina seruma: poredenje metoda sa tiobarbiturnom kiselinom i fenol-sumpornom kiselinomsr
dc.titleDetermination of serum glycosylated proteins: Comparison of thiobarbituric acid and phenol-sulphuric acid assaysen
dc.typearticlesr
dc.rights.licenseBYsr
dcterms.abstractМилин, Ненад; Стојановић, Срђан; Чоловић, Aна; Мандић, Љуба; Одредивање гликозилованих протеина серума: поредење метода са тиобарбитурном киселином и фенол-сумпорном киселином; Одредивање гликозилованих протеина серума: поредење метода са тиобарбитурном киселином и фенол-сумпорном киселином;
dc.citation.volume19
dc.citation.issue4
dc.citation.spage411
dc.citation.epage416
dc.citation.rankM23
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/18028/JMedBioh2000.pdf
dc.identifier.scopus2-s2.0-0034496242
dc.identifier.wos000166041900007
dc.type.versionpublishedVersionsr


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