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A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood

Spektrofotometrijsko određivanje acetaldehida u krvi sa tiosemikarbazidom

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1998
phat98-09.pdf (190.7Kb)
Authors
Đurđić, Vidosava
Stojanović, Srđan
Article (Published version)
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Abstract
The thiosemicarbazide method for spectrophotometric determination of acetaldehyde (AcH) in whole blood is presented. The concentration of thiosemicarbazone formed in slightly acidic medium was determined at 260 nm. The influence of non-specific chromogens present in blood was eliminated by simultaneous AcH distillation and thiosemicarbazone formation. The oxidation of AcH in blood samples was prevented by chloral hydrate addition. The presented method was found two folds more sensitive than a spectrophotometric determination of AcH by semicarbazide. Due to its simplicity and sensitivity as well as to the use of low cost equipment, this method should be a valuable complement to expensive GLC or HPLC determinations of AcH.
U radu je opisana metoda za spektrofotometrijsko određivanje acetaldehida (AcH) u krvi. Kao reagens upotrebljen je tiosemikarbazid a apsorpcija nastalih u slabo kiseloj sredini tiosemikarbazona određuje se na 260 nm. Uticaj nespecifičnih hromogena prisutnih u krvi eliminiše se destilacijom AcH sa istovremenim građenjem tiosemikarbazona. Oksidacija AcH u uzorcima krvi sprečena je dodatkom hloral hidrata. Opisana metoda se pokazala osetljivom u odnosu na spektrofotometrijsko određivanje AcH sa semikarbazidom. Zbog svoje jednostavnosti, osetljivosti i ekonomičnosti ova metoda može predstavljati korisnu zamenu za GLC i HPLC određivanja AcH.
Keywords:
Acetaldehyde (AcH) / Thiosemicarbazide / Spectrophotometric determination / Thiosemicarbazone / Acetildehid (AcH) / Tiosemikarbazid / Spektrofotometrijsko određivanje / Tiosemikarbazon
Source:
Facta Universitatis (Physics, Chemistry and Technology), 1998, 1, 5, 129-134
Publisher:
  • Niš, Serbia : University of Niš

ISSN: 0354-4656

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_cer_3888
URI
http://facta.junis.ni.ac.rs/phat/phat98/phat98-09.pdf
https://cer.ihtm.bg.ac.rs/handle/123456789/3888
Collections
  • Radovi istraživača pre dolaska u IHTM / Researchers' papers before joining ICTM
Institution/Community
IHTM
TY  - JOUR
AU  - Đurđić, Vidosava
AU  - Stojanović, Srđan
PY  - 1998
UR  - http://facta.junis.ni.ac.rs/phat/phat98/phat98-09.pdf
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3888
AB  - The  thiosemicarbazide  method  for  spectrophotometric  determination  of  acetaldehyde    (AcH)    in    whole    blood    is    presented.    The    concentration    of    thiosemicarbazone  formed  in  slightly  acidic  medium  was  determined  at  260  nm.  The  influence of non-specific chromogens present in blood was eliminated by simultaneous AcH  distillation  and  thiosemicarbazone  formation.  The  oxidation  of  AcH  in  blood  samples  was  prevented  by  chloral  hydrate  addition.  The  presented  method  was  found  two   folds   more   sensitive   than   a   spectrophotometric   determination   of   AcH   by   semicarbazide.  Due  to  its  simplicity  and  sensitivity  as  well  as  to  the  use  of  low  cost  equipment,  this  method  should  be  a  valuable  complement  to  expensive  GLC  or  HPLC  determinations of AcH.
AB  - U radu je opisana metoda za spektrofotometrijsko određivanje acetaldehida (AcH) u krvi. Kao
reagens upotrebljen je tiosemikarbazid a apsorpcija nastalih u slabo kiseloj sredini
tiosemikarbazona određuje se na 260 nm. Uticaj nespecifičnih hromogena prisutnih u krvi
eliminiše se destilacijom AcH sa istovremenim građenjem tiosemikarbazona. Oksidacija AcH u
uzorcima krvi sprečena je dodatkom hloral hidrata. Opisana metoda se pokazala osetljivom u
odnosu na spektrofotometrijsko određivanje AcH sa semikarbazidom. Zbog svoje jednostavnosti,
osetljivosti i ekonomičnosti ova metoda može predstavljati korisnu zamenu za GLC i HPLC
određivanja AcH.
PB  - Niš, Serbia : University of Niš
T2  - Facta Universitatis (Physics, Chemistry and Technology)
T1  - A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood
T1  - Spektrofotometrijsko određivanje acetaldehida u krvi sa tiosemikarbazidom
VL  - 1
IS  - 5
SP  - 129
EP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_cer_3888
ER  - 
@article{
author = "Đurđić, Vidosava and Stojanović, Srđan",
year = "1998",
abstract = "The  thiosemicarbazide  method  for  spectrophotometric  determination  of  acetaldehyde    (AcH)    in    whole    blood    is    presented.    The    concentration    of    thiosemicarbazone  formed  in  slightly  acidic  medium  was  determined  at  260  nm.  The  influence of non-specific chromogens present in blood was eliminated by simultaneous AcH  distillation  and  thiosemicarbazone  formation.  The  oxidation  of  AcH  in  blood  samples  was  prevented  by  chloral  hydrate  addition.  The  presented  method  was  found  two   folds   more   sensitive   than   a   spectrophotometric   determination   of   AcH   by   semicarbazide.  Due  to  its  simplicity  and  sensitivity  as  well  as  to  the  use  of  low  cost  equipment,  this  method  should  be  a  valuable  complement  to  expensive  GLC  or  HPLC  determinations of AcH., U radu je opisana metoda za spektrofotometrijsko određivanje acetaldehida (AcH) u krvi. Kao
reagens upotrebljen je tiosemikarbazid a apsorpcija nastalih u slabo kiseloj sredini
tiosemikarbazona određuje se na 260 nm. Uticaj nespecifičnih hromogena prisutnih u krvi
eliminiše se destilacijom AcH sa istovremenim građenjem tiosemikarbazona. Oksidacija AcH u
uzorcima krvi sprečena je dodatkom hloral hidrata. Opisana metoda se pokazala osetljivom u
odnosu na spektrofotometrijsko određivanje AcH sa semikarbazidom. Zbog svoje jednostavnosti,
osetljivosti i ekonomičnosti ova metoda može predstavljati korisnu zamenu za GLC i HPLC
određivanja AcH.",
publisher = "Niš, Serbia : University of Niš",
journal = "Facta Universitatis (Physics, Chemistry and Technology)",
title = "A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood, Spektrofotometrijsko određivanje acetaldehida u krvi sa tiosemikarbazidom",
volume = "1",
number = "5",
pages = "129-134",
url = "https://hdl.handle.net/21.15107/rcub_cer_3888"
}
Đurđić, V.,& Stojanović, S.. (1998). A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood. in Facta Universitatis (Physics, Chemistry and Technology)
Niš, Serbia : University of Niš., 1(5), 129-134.
https://hdl.handle.net/21.15107/rcub_cer_3888
Đurđić V, Stojanović S. A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood. in Facta Universitatis (Physics, Chemistry and Technology). 1998;1(5):129-134.
https://hdl.handle.net/21.15107/rcub_cer_3888 .
Đurđić, Vidosava, Stojanović, Srđan, "A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood" in Facta Universitatis (Physics, Chemistry and Technology), 1, no. 5 (1998):129-134,
https://hdl.handle.net/21.15107/rcub_cer_3888 .

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