The thiosemicarbazide method for spectrophotometric determination of acetaldehyde (AcH) in whole blood is presented. The concentration of thiosemicarbazone formed in slightly acidic medium was determined at 260 nm. The influence of non-specific chromogens present in blood was eliminated by simultaneous AcH distillation and thiosemicarbazone formation. The oxidation of AcH in blood samples was prevented by chloral hydrate addition. The presented method was found two folds more sensitive than a spectrophotometric determination of AcH by semicarbazide. Due to its simplicity and sensitivity as well as to the use of low cost equipment, this method should be a valuable complement to expensive GLC or HPLC determinations of AcH.
U radu je opisana metoda za spektrofotometrijsko određivanje acetaldehida (AcH) u krvi. Kao
reagens upotrebljen je tiosemikarbazid a apsorpcija nastalih u slabo kiseloj sredini
tiosemikarbazona određuje se na 260 nm. Uticaj nespecifičnih hromogena prisutnih u krvi
eliminiše se destilacijom AcH sa istovremenim građenjem tiosemikarbazona. Oksidacija AcH u
uzorcima krvi sprečena je dodatkom hloral hidrata. Opisana metoda se pokazala osetljivom u
odnosu na spektrofotometrijsko određivanje AcH sa semikarbazidom. Zbog svoje jednostavnosti,
osetljivosti i ekonomičnosti ova metoda može predstavljati korisnu zamenu za GLC i HPLC
određivanja AcH.
TY - JOUR
AU - Đurđić, Vidosava
AU - Stojanović, Srđan
PY - 1998
UR - http://facta.junis.ni.ac.rs/phat/phat98/phat98-09.pdf
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3888
AB - The thiosemicarbazide method for spectrophotometric determination of acetaldehyde (AcH) in whole blood is presented. The concentration of thiosemicarbazone formed in slightly acidic medium was determined at 260 nm. The influence of non-specific chromogens present in blood was eliminated by simultaneous AcH distillation and thiosemicarbazone formation. The oxidation of AcH in blood samples was prevented by chloral hydrate addition. The presented method was found two folds more sensitive than a spectrophotometric determination of AcH by semicarbazide. Due to its simplicity and sensitivity as well as to the use of low cost equipment, this method should be a valuable complement to expensive GLC or HPLC determinations of AcH.
AB - U radu je opisana metoda za spektrofotometrijsko određivanje acetaldehida (AcH) u krvi. Kao
reagens upotrebljen je tiosemikarbazid a apsorpcija nastalih u slabo kiseloj sredini
tiosemikarbazona određuje se na 260 nm. Uticaj nespecifičnih hromogena prisutnih u krvi
eliminiše se destilacijom AcH sa istovremenim građenjem tiosemikarbazona. Oksidacija AcH u
uzorcima krvi sprečena je dodatkom hloral hidrata. Opisana metoda se pokazala osetljivom u
odnosu na spektrofotometrijsko određivanje AcH sa semikarbazidom. Zbog svoje jednostavnosti,
osetljivosti i ekonomičnosti ova metoda može predstavljati korisnu zamenu za GLC i HPLC
određivanja AcH.
PB - Niš, Serbia : University of Niš
T2 - Facta Universitatis (Physics, Chemistry and Technology)
T1 - A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood
T1 - Spektrofotometrijsko određivanje acetaldehida u krvi sa tiosemikarbazidom
VL - 1
IS - 5
SP - 129
EP - 134
ER -
@article{
author = "Đurđić, Vidosava and Stojanović, Srđan",
year = "1998",
abstract = "The thiosemicarbazide method for spectrophotometric determination of acetaldehyde (AcH) in whole blood is presented. The concentration of thiosemicarbazone formed in slightly acidic medium was determined at 260 nm. The influence of non-specific chromogens present in blood was eliminated by simultaneous AcH distillation and thiosemicarbazone formation. The oxidation of AcH in blood samples was prevented by chloral hydrate addition. The presented method was found two folds more sensitive than a spectrophotometric determination of AcH by semicarbazide. Due to its simplicity and sensitivity as well as to the use of low cost equipment, this method should be a valuable complement to expensive GLC or HPLC determinations of AcH., U radu je opisana metoda za spektrofotometrijsko određivanje acetaldehida (AcH) u krvi. Kao
reagens upotrebljen je tiosemikarbazid a apsorpcija nastalih u slabo kiseloj sredini
tiosemikarbazona određuje se na 260 nm. Uticaj nespecifičnih hromogena prisutnih u krvi
eliminiše se destilacijom AcH sa istovremenim građenjem tiosemikarbazona. Oksidacija AcH u
uzorcima krvi sprečena je dodatkom hloral hidrata. Opisana metoda se pokazala osetljivom u
odnosu na spektrofotometrijsko određivanje AcH sa semikarbazidom. Zbog svoje jednostavnosti,
osetljivosti i ekonomičnosti ova metoda može predstavljati korisnu zamenu za GLC i HPLC
određivanja AcH.",
publisher = "Niš, Serbia : University of Niš",
journal = "Facta Universitatis (Physics, Chemistry and Technology)",
title = "A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood, Spektrofotometrijsko određivanje acetaldehida u krvi sa tiosemikarbazidom",
volume = "1",
number = "5",
pages = "129-134"
}
Đurđić, V.,& Stojanović, S.. (1998). A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood. in Facta Universitatis (Physics, Chemistry and Technology)
Niš, Serbia : University of Niš., 1(5), 129-134.
Đurđić V, Stojanović S. A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood. in Facta Universitatis (Physics, Chemistry and Technology). 1998;1(5):129-134..
Đurđić, Vidosava, Stojanović, Srđan, "A method for spectrophotometric determination of acetaldehyde with thiosemicarbazide in blood" in Facta Universitatis (Physics, Chemistry and Technology), 1, no. 5 (1998):129-134.
