Genetic and morphological variation in Chara contraria and a taxon morphologically resembling Chara connivens
Authorized Users Only
2020
Authors
Trbojević, Ivana
Marković, Aleksandra

Blaženčić, Jelena
Subakov Simić, Gordana

Nowak, Petra
Ballot, Andreas
Schneider, Susanne
Article (Published version)

Société botanique de France
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Show full item recordAbstract
Charophyte species delineation is regularly based on a set of thallus morphological character- istics, but considering pronounced phenotypic plasticity, difficulties and doubts commonly occur in Chara species determination. DNA barcoding may contribute to solving these chal- lenges. Here we characterize Chara contraria with an unusual set of morphological character- istics, and specimens morphologically resembling Chara connivens collected in Serbia, by describing their morphological traits and analysing matK barcoding results. Our results indi- cated that dioecious Chara specimens, tentatively determined as Chara “connivens” based on morphological traits, were genetically more closely related to C. globularis. These Chara
“connivens” specimens formed a sister group to a monophyletic C. globularis cluster, suggesting that it may be neither C. connivens nor C. globularis. We strongly encourage further barcoding of C. “connivens” samples from freshwater, in order to find out if there ar...e consistent genetic differences between the dioecious freshwater C. “connivens” and monoecious C. globularis. Barcoding of matK placed the monoecious Chara specimens, which based on morphological characteristics initially were determined as C. virgata, into the C. contraria group. This indicates that the microscopic traits which commonly are used for Chara species determination some- times are misleading. In general, our study challenges the commonly used phenetic species concept in Charophyte taxonomy and illustrates the importance of molecular approaches to evaluate the validity of morphological characteristics of the plant thallus in species delineation.
Keywords:
algae / barcode / Charales / charophyte / matKSource:
Botany Letters, 2020, 167, 2, 187-200Publisher:
- Taylor & Francis
Funding / projects:
- Transformation of Serbian Geospace - lessons from the past, contemporary problems and possible solutions (RS-176020)
- Norwegian Ministry of Foreign Affairs [STAR-WALK project]
- Rufford Foundation [25789-1]
DOI: 10.1080/23818107.2019.1703808
ISSN: 2381-8107; 2381-8115 (Online)
WoS: 000506150300001
Scopus: 2-s2.0-85077862586
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IHTMTY - JOUR AU - Trbojević, Ivana AU - Marković, Aleksandra AU - Blaženčić, Jelena AU - Subakov Simić, Gordana AU - Nowak, Petra AU - Ballot, Andreas AU - Schneider, Susanne PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3865 AB - Charophyte species delineation is regularly based on a set of thallus morphological character- istics, but considering pronounced phenotypic plasticity, difficulties and doubts commonly occur in Chara species determination. DNA barcoding may contribute to solving these chal- lenges. Here we characterize Chara contraria with an unusual set of morphological character- istics, and specimens morphologically resembling Chara connivens collected in Serbia, by describing their morphological traits and analysing matK barcoding results. Our results indi- cated that dioecious Chara specimens, tentatively determined as Chara “connivens” based on morphological traits, were genetically more closely related to C. globularis. These Chara “connivens” specimens formed a sister group to a monophyletic C. globularis cluster, suggesting that it may be neither C. connivens nor C. globularis. We strongly encourage further barcoding of C. “connivens” samples from freshwater, in order to find out if there are consistent genetic differences between the dioecious freshwater C. “connivens” and monoecious C. globularis. Barcoding of matK placed the monoecious Chara specimens, which based on morphological characteristics initially were determined as C. virgata, into the C. contraria group. This indicates that the microscopic traits which commonly are used for Chara species determination some- times are misleading. In general, our study challenges the commonly used phenetic species concept in Charophyte taxonomy and illustrates the importance of molecular approaches to evaluate the validity of morphological characteristics of the plant thallus in species delineation. PB - Taylor & Francis T2 - Botany Letters T1 - Genetic and morphological variation in Chara contraria and a taxon morphologically resembling Chara connivens VL - 167 IS - 2 SP - 187 EP - 200 DO - 10.1080/23818107.2019.1703808 ER -
@article{ author = "Trbojević, Ivana and Marković, Aleksandra and Blaženčić, Jelena and Subakov Simić, Gordana and Nowak, Petra and Ballot, Andreas and Schneider, Susanne", year = "2020", abstract = "Charophyte species delineation is regularly based on a set of thallus morphological character- istics, but considering pronounced phenotypic plasticity, difficulties and doubts commonly occur in Chara species determination. DNA barcoding may contribute to solving these chal- lenges. Here we characterize Chara contraria with an unusual set of morphological character- istics, and specimens morphologically resembling Chara connivens collected in Serbia, by describing their morphological traits and analysing matK barcoding results. Our results indi- cated that dioecious Chara specimens, tentatively determined as Chara “connivens” based on morphological traits, were genetically more closely related to C. globularis. These Chara “connivens” specimens formed a sister group to a monophyletic C. globularis cluster, suggesting that it may be neither C. connivens nor C. globularis. We strongly encourage further barcoding of C. “connivens” samples from freshwater, in order to find out if there are consistent genetic differences between the dioecious freshwater C. “connivens” and monoecious C. globularis. Barcoding of matK placed the monoecious Chara specimens, which based on morphological characteristics initially were determined as C. virgata, into the C. contraria group. This indicates that the microscopic traits which commonly are used for Chara species determination some- times are misleading. In general, our study challenges the commonly used phenetic species concept in Charophyte taxonomy and illustrates the importance of molecular approaches to evaluate the validity of morphological characteristics of the plant thallus in species delineation.", publisher = "Taylor & Francis", journal = "Botany Letters", title = "Genetic and morphological variation in Chara contraria and a taxon morphologically resembling Chara connivens", volume = "167", number = "2", pages = "187-200", doi = "10.1080/23818107.2019.1703808" }
Trbojević, I., Marković, A., Blaženčić, J., Subakov Simić, G., Nowak, P., Ballot, A.,& Schneider, S.. (2020). Genetic and morphological variation in Chara contraria and a taxon morphologically resembling Chara connivens. in Botany Letters Taylor & Francis., 167(2), 187-200. https://doi.org/10.1080/23818107.2019.1703808
Trbojević I, Marković A, Blaženčić J, Subakov Simić G, Nowak P, Ballot A, Schneider S. Genetic and morphological variation in Chara contraria and a taxon morphologically resembling Chara connivens. in Botany Letters. 2020;167(2):187-200. doi:10.1080/23818107.2019.1703808 .
Trbojević, Ivana, Marković, Aleksandra, Blaženčić, Jelena, Subakov Simić, Gordana, Nowak, Petra, Ballot, Andreas, Schneider, Susanne, "Genetic and morphological variation in Chara contraria and a taxon morphologically resembling Chara connivens" in Botany Letters, 167, no. 2 (2020):187-200, https://doi.org/10.1080/23818107.2019.1703808 . .