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dc.creatorVujčić, Miroslava
dc.creatorVeličković, N.
dc.creatorRuždijić, S.
dc.date.accessioned2019-01-30T17:16:50Z
dc.date.available2019-01-30T17:16:50Z
dc.date.issued2007
dc.identifier.issn1096-4959
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/373
dc.description.abstractAging is associated with marked changes in the biochemical processes of many organs. Basal and glucocorticoid induced of liver nuclear glucocorticoid receptor (GR) on the level of protein expression and DNA-binding activity were investigated at different ages (3, 6, 12, 18 and 24 months old) in two groups of rats in: untreated and dexamethasone treated. The results showed a significant decline of GR protein immunopurified from untreated rats of advanced age. In dexamethasone-treated rats, the quantity of GR protein was lower than in controls at all ages. The interactions of liver nuclear proteins with radioactively labelled synthetic oligonucleotide analogue containing consensus GRE sequence were analysed during aging. The results showed that GRE binding activity demonstrated a decrease both in untreated and in dexamethasone treated rats. However, relative to untreated rats, dexamethasone treatment resulted in a significant increase in GRE binding at all ages, except that of three months old animals. In conclusion, the observed alterations in GR protein expression and its DNA binding activity may play a role in the changes of the cell response to glucocorticoid during aging.en
dc.publisherElsevier Science Inc, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143044/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143009/RS//
dc.rightsrestrictedAccess
dc.sourceComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
dc.subjectAgingen
dc.subjectDexamethasoneen
dc.subjectDNA-protein interactionen
dc.subjectGlucocorticoid receptoren
dc.subjectGREen
dc.titleDexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during agingen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВеличковић, Н.; Вујчић, Мирослава; Руждијић, С.;
dc.citation.volume148
dc.citation.issue4
dc.citation.spage463
dc.citation.epage469
dc.citation.other148(4): 463-469
dc.citation.rankM21
dc.identifier.pmid17761446
dc.identifier.doi10.1016/j.cbpb.2007.07.090
dc.identifier.rcubConv_4099
dc.identifier.scopus2-s2.0-35649002620
dc.identifier.wos000251481700013
dc.type.versionpublishedVersion


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