JavaScript is disabled for your browser. Some features of this site may not work without it.
Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging
Aging is associated with marked changes in the biochemical processes of many organs. Basal and glucocorticoid induced of liver nuclear glucocorticoid receptor (GR) on the level of protein expression and DNA-binding activity were investigated at different ages (3, 6, 12, 18 and 24 months old) in two groups of rats in: untreated and dexamethasone treated. The results showed a significant decline of GR protein immunopurified from untreated rats of advanced age. In dexamethasone-treated rats, the quantity of GR protein was lower than in controls at all ages. The interactions of liver nuclear proteins with radioactively labelled synthetic oligonucleotide analogue containing consensus GRE sequence were analysed during aging. The results showed that GRE binding activity demonstrated a decrease both in untreated and in dexamethasone treated rats. However, relative to untreated rats, dexamethasone treatment resulted in a significant increase in GRE binding at all ages, except that of three mont...hs old animals. In conclusion, the observed alterations in GR protein expression and its DNA binding activity may play a role in the changes of the cell response to glucocorticoid during aging.
TY - JOUR
AU - Vujčić, Miroslava
AU - Veličković, N.
AU - Ruždijić, S.
PY - 2007
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/373
AB - Aging is associated with marked changes in the biochemical processes of many organs. Basal and glucocorticoid induced of liver nuclear glucocorticoid receptor (GR) on the level of protein expression and DNA-binding activity were investigated at different ages (3, 6, 12, 18 and 24 months old) in two groups of rats in: untreated and dexamethasone treated. The results showed a significant decline of GR protein immunopurified from untreated rats of advanced age. In dexamethasone-treated rats, the quantity of GR protein was lower than in controls at all ages. The interactions of liver nuclear proteins with radioactively labelled synthetic oligonucleotide analogue containing consensus GRE sequence were analysed during aging. The results showed that GRE binding activity demonstrated a decrease both in untreated and in dexamethasone treated rats. However, relative to untreated rats, dexamethasone treatment resulted in a significant increase in GRE binding at all ages, except that of three months old animals. In conclusion, the observed alterations in GR protein expression and its DNA binding activity may play a role in the changes of the cell response to glucocorticoid during aging.
PB - Elsevier Science Inc, New York
T2 - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
T1 - Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging
VL - 148
IS - 4
SP - 463
EP - 469
DO - 10.1016/j.cbpb.2007.07.090
ER -
@article{
author = "Vujčić, Miroslava and Veličković, N. and Ruždijić, S.",
year = "2007",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/373",
abstract = "Aging is associated with marked changes in the biochemical processes of many organs. Basal and glucocorticoid induced of liver nuclear glucocorticoid receptor (GR) on the level of protein expression and DNA-binding activity were investigated at different ages (3, 6, 12, 18 and 24 months old) in two groups of rats in: untreated and dexamethasone treated. The results showed a significant decline of GR protein immunopurified from untreated rats of advanced age. In dexamethasone-treated rats, the quantity of GR protein was lower than in controls at all ages. The interactions of liver nuclear proteins with radioactively labelled synthetic oligonucleotide analogue containing consensus GRE sequence were analysed during aging. The results showed that GRE binding activity demonstrated a decrease both in untreated and in dexamethasone treated rats. However, relative to untreated rats, dexamethasone treatment resulted in a significant increase in GRE binding at all ages, except that of three months old animals. In conclusion, the observed alterations in GR protein expression and its DNA binding activity may play a role in the changes of the cell response to glucocorticoid during aging.",
publisher = "Elsevier Science Inc, New York",
journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",
title = "Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging",
volume = "148",
number = "4",
pages = "463-469",
doi = "10.1016/j.cbpb.2007.07.090"
}
Vujčić, M., Veličković, N.,& Ruždijić, S. (2007). Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging.
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Elsevier Science Inc, New York., 148(4), 463-469.
https://doi.org/10.1016/j.cbpb.2007.07.090
Vujčić M, Veličković N, Ruždijić S. Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 2007;148(4):463-469
Vujčić Miroslava, Veličković N., Ruždijić S., "Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging" Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 148, no. 4 (2007):463-469,
https://doi.org/10.1016/j.cbpb.2007.07.090 .
