Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals
Authors
Nikolić, Ines
Antić-Stanković, Jelena

Božić, Dragana

Randjelović, Danijela

Marković, Bojan D.

Lunter, Dominique Jasmin

Kremenović, Aleksandar

Savić, Miroslav M.

Savić, Snežana

Article (Published version)
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As the number of poorly soluble drugs is increasing, nanocrystals have become very interesting due to wide range of application possibilities. Curcuminwas used as a model active ingredient in this work. Even though it has many proven positive effects, due to its physicochemical issues, its possibilities have not been fully exploited. The goal of this work was to select optimal conditions for a top-down method for curcumin nanosuspension production, and to perform their comprehensive characterization applying complementary methodologies: dynamic light scattering, polarization and atomic force microscopy, thermal analysis, X-ray powder diffraction, antioxidant activity evaluation, release kinetics assessment, and screening of potential biological effects applying cell viability assays on normal human lung fibroblasts, human melanoma and human adenomacarcinoma cells. After 30 min of milling, nanosuspensions stabilized by polysorbate 80 and by its combinations with sucrose palmitate showed... good stability, while curcumin crystal structure was unaltered. Obtained nanocrystals were well defined, with average diameter 120-170 nm and PDI of about 0.25, zeta potential was below -30 mV and pH~5 for all formulations. Nanodispersions exhibited high antioxidant potential and improved dissolution rate compared to the corresponding coarse dispersions. Although curcumin nanodispersions exhibited significant antiproliferative effect to each cancer cell line, the highest effect was towards adenocarcinoma cells.
Keywords:
curcumin / nanocrystal / top-down method / wetball millingSource:
Reviews on Advanced Materials Science, 2020, 59, 1, 406-424Publisher:
- Walter de Gruyter GmbH
Funding / projects:
DOI: 10.1515/rams-2020-0043
ISSN: 1606-5131
WoS: 000579065000001
Scopus: 2-s2.0-85092928784
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/3711https://cer.ihtm.bg.ac.rs/handle/123456789/3720
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IHTMTY - JOUR AU - Nikolić, Ines AU - Antić-Stanković, Jelena AU - Božić, Dragana AU - Randjelović, Danijela AU - Marković, Bojan D. AU - Lunter, Dominique Jasmin AU - Kremenović, Aleksandar AU - Savić, Miroslav M. AU - Savić, Snežana PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3711 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3720 AB - As the number of poorly soluble drugs is increasing, nanocrystals have become very interesting due to wide range of application possibilities. Curcuminwas used as a model active ingredient in this work. Even though it has many proven positive effects, due to its physicochemical issues, its possibilities have not been fully exploited. The goal of this work was to select optimal conditions for a top-down method for curcumin nanosuspension production, and to perform their comprehensive characterization applying complementary methodologies: dynamic light scattering, polarization and atomic force microscopy, thermal analysis, X-ray powder diffraction, antioxidant activity evaluation, release kinetics assessment, and screening of potential biological effects applying cell viability assays on normal human lung fibroblasts, human melanoma and human adenomacarcinoma cells. After 30 min of milling, nanosuspensions stabilized by polysorbate 80 and by its combinations with sucrose palmitate showed good stability, while curcumin crystal structure was unaltered. Obtained nanocrystals were well defined, with average diameter 120-170 nm and PDI of about 0.25, zeta potential was below -30 mV and pH~5 for all formulations. Nanodispersions exhibited high antioxidant potential and improved dissolution rate compared to the corresponding coarse dispersions. Although curcumin nanodispersions exhibited significant antiproliferative effect to each cancer cell line, the highest effect was towards adenocarcinoma cells. PB - Walter de Gruyter GmbH T2 - Reviews on Advanced Materials Science T1 - Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals VL - 59 IS - 1 SP - 406 EP - 424 DO - 10.1515/rams-2020-0043 ER -
@article{ author = "Nikolić, Ines and Antić-Stanković, Jelena and Božić, Dragana and Randjelović, Danijela and Marković, Bojan D. and Lunter, Dominique Jasmin and Kremenović, Aleksandar and Savić, Miroslav M. and Savić, Snežana", year = "2020", abstract = "As the number of poorly soluble drugs is increasing, nanocrystals have become very interesting due to wide range of application possibilities. Curcuminwas used as a model active ingredient in this work. Even though it has many proven positive effects, due to its physicochemical issues, its possibilities have not been fully exploited. The goal of this work was to select optimal conditions for a top-down method for curcumin nanosuspension production, and to perform their comprehensive characterization applying complementary methodologies: dynamic light scattering, polarization and atomic force microscopy, thermal analysis, X-ray powder diffraction, antioxidant activity evaluation, release kinetics assessment, and screening of potential biological effects applying cell viability assays on normal human lung fibroblasts, human melanoma and human adenomacarcinoma cells. After 30 min of milling, nanosuspensions stabilized by polysorbate 80 and by its combinations with sucrose palmitate showed good stability, while curcumin crystal structure was unaltered. Obtained nanocrystals were well defined, with average diameter 120-170 nm and PDI of about 0.25, zeta potential was below -30 mV and pH~5 for all formulations. Nanodispersions exhibited high antioxidant potential and improved dissolution rate compared to the corresponding coarse dispersions. Although curcumin nanodispersions exhibited significant antiproliferative effect to each cancer cell line, the highest effect was towards adenocarcinoma cells.", publisher = "Walter de Gruyter GmbH", journal = "Reviews on Advanced Materials Science", title = "Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals", volume = "59", number = "1", pages = "406-424", doi = "10.1515/rams-2020-0043" }
Nikolić, I., Antić-Stanković, J., Božić, D., Randjelović, D., Marković, B. D., Lunter, D. J., Kremenović, A., Savić, M. M.,& Savić, S.. (2020). Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals. in Reviews on Advanced Materials Science Walter de Gruyter GmbH., 59(1), 406-424. https://doi.org/10.1515/rams-2020-0043
Nikolić I, Antić-Stanković J, Božić D, Randjelović D, Marković BD, Lunter DJ, Kremenović A, Savić MM, Savić S. Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals. in Reviews on Advanced Materials Science. 2020;59(1):406-424. doi:10.1515/rams-2020-0043 .
Nikolić, Ines, Antić-Stanković, Jelena, Božić, Dragana, Randjelović, Danijela, Marković, Bojan D., Lunter, Dominique Jasmin, Kremenović, Aleksandar, Savić, Miroslav M., Savić, Snežana, "Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals" in Reviews on Advanced Materials Science, 59, no. 1 (2020):406-424, https://doi.org/10.1515/rams-2020-0043 . .