Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin
Autori
Batinić, Petar M.Đorđević, Verica B.
Stevanović, Sanja
Balanč, Bojana D.
Marković, Smilja
Luković, Nevena
Mijin, Dušan
Bugarski, Branko
Članak u časopisu (Recenzirana verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Releas...e study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.
Ključne reči:
Encapsulation efficiency / Phospholipon 90G / Cholesterol / SPAN.20 / Franz diffusion cellIzvor:
Journal of Drug Delivery Science and Technology, 2020, 59, 101920-Izdavač:
- Elsevier
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-200287)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-200135)
Napomena:
- This is the peer-reviewed version of the article: P.M. Batinić, V.B. Đorđević, S.I. Stevanović, B.D. Balanč, S.B. Marković, N.D. Luković, D.Ž. Mijin, B.M. Bugarski, Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin, Journal of Drug Delivery Science and Technology, 2020, 59, 101920, doi: https://doi.org/10.1016/j.jddst.2020.101920
- The published version: http://cer.ihtm.bg.ac.rs/handle/123456789/3681
DOI: 10.1016/j.jddst.2020.101920
ISSN: 1773-2247
WoS: 000572445500003
Scopus: 2-s2.0-85088379189
Institucija/grupa
IHTMTY - JOUR AU - Batinić, Petar M. AU - Đorđević, Verica B. AU - Stevanović, Sanja AU - Balanč, Bojana D. AU - Marković, Smilja AU - Luković, Nevena AU - Mijin, Dušan AU - Bugarski, Branko PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3682 AB - In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Release study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility. PB - Elsevier T2 - Journal of Drug Delivery Science and Technology T1 - Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin VL - 59 SP - 101920 DO - 10.1016/j.jddst.2020.101920 ER -
@article{ author = "Batinić, Petar M. and Đorđević, Verica B. and Stevanović, Sanja and Balanč, Bojana D. and Marković, Smilja and Luković, Nevena and Mijin, Dušan and Bugarski, Branko", year = "2020", abstract = "In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Release study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.", publisher = "Elsevier", journal = "Journal of Drug Delivery Science and Technology", title = "Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin", volume = "59", pages = "101920", doi = "10.1016/j.jddst.2020.101920" }
Batinić, P. M., Đorđević, V. B., Stevanović, S., Balanč, B. D., Marković, S., Luković, N., Mijin, D.,& Bugarski, B.. (2020). Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin. in Journal of Drug Delivery Science and Technology Elsevier., 59, 101920. https://doi.org/10.1016/j.jddst.2020.101920
Batinić PM, Đorđević VB, Stevanović S, Balanč BD, Marković S, Luković N, Mijin D, Bugarski B. Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin. in Journal of Drug Delivery Science and Technology. 2020;59:101920. doi:10.1016/j.jddst.2020.101920 .
Batinić, Petar M., Đorđević, Verica B., Stevanović, Sanja, Balanč, Bojana D., Marković, Smilja, Luković, Nevena, Mijin, Dušan, Bugarski, Branko, "Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin" in Journal of Drug Delivery Science and Technology, 59 (2020):101920, https://doi.org/10.1016/j.jddst.2020.101920 . .