Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin
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AuthorsBatinić, Petar M.
Đorđević, Verica B.
Balanč, Bojana D.
Luković, Nevena D.
Article (Accepted Version)
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In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Releas...e study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.
Keywords:Encapsulation efficiency / Phospholipon 90G / Cholesterol / SPAN.20 / Franz diffusion cell
Source:Journal of Drug Delivery Science and Technology, 2020, 59, 101920-
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-200287)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-200135)
- This is the peer-reviewed version of the article: P.M. Batinić, V.B. Đorđević, S.I. Stevanović, B.D. Balanč, S.B. Marković, N.D. Luković, D.Ž. Mijin, B.M. Bugarski, Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin, Journal of Drug Delivery Science and Technology, 2020, 59, 101920, doi: https://doi.org/10.1016/j.jddst.2020.101920
- The published version: http://cer.ihtm.bg.ac.rs/handle/123456789/3681