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dc.creatorMilovanović, Aleksandra
dc.creatorBožić, Nataša
dc.creatorVujčić, Zoran
dc.date.accessioned2019-01-30T17:16:41Z
dc.date.available2019-01-30T17:16:41Z
dc.date.issued2007
dc.identifier.issn0308-8146
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/365
dc.description.abstractYeast cell wall invertase (CWI) was immobilized within calcium alginate beads. The result of entrapment was the complete immobilization of all the added CWI. Three types of biocatalysts were prepared: CWI-S, CWI-155 and CWI-157. The optimum pH values were 4.5 and 5.0 for free and immobilized invertase, respectively. The optimum temperature was 60 °C, for both free CWI and CWI-S immobilizate. 80 °C was the optimum temperature for CWI-155 whereas the optimum temperature for CWI-157 ranged from 50 °C to 80 °C. Immobilized CWI was more stable than was free CWI above optimum activity temperatures. The activation energies were 32 kJ/mol for free CWI and 45, 21 and 25 kJ/mol for CWI-S, CWI-155 and CWI-157, respectively. The Km values of free and immobilized CWI-157 were 28.4 mM and 72 mM, respectively. The Vmax values were estimated as 4.5 mM/min and 0.42 mM/min, respectively. Immobilized CWI-157 was tested in a batch reactor using 70% sucrose (w/v). Complete sucrose conversion was achieved after 55 h. After 40 consecutive cycles, CWI-157 retained 90% of its activity.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142026/RS//
dc.rightsrestrictedAccess
dc.sourceFood Chemistry
dc.subjectAlginateen
dc.subjectCell wall invertaseen
dc.subjectImmobilizationen
dc.subjectSaccharomyces cerevisiaeen
dc.titleCell wall invertase immobilization within calcium alginate beadsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВујчић, З.; Милутиновић Николић, Aлександра; Божић, Наташа;
dc.citation.volume104
dc.citation.issue1
dc.citation.spage81
dc.citation.epage86
dc.citation.other104(1): 81-86
dc.citation.rankaM21
dc.identifier.doi10.1016/j.foodchem.2006.11.001
dc.identifier.rcubConv_4065
dc.identifier.scopus2-s2.0-34147160238
dc.identifier.wos000246630500011
dc.type.versionpublishedVersion


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