Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity
Authors
Medić, AnaLješević, Marija
Inui, Hideyuku
Beškoski, Vladimir
Kojić, Ivan
Stojanović, Ksenija
Karadžić, Ivanka
Article (Published version)
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Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected
petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic
hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%,
respectively, at initial concentrations of 20 mg L 1 and in seven days. P. aeruginosa san ai showed a high
biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from
crude oil. The efficiency of P. aeruginosa san ai degradation of crude oil fractions in seven days reached
stage 3–4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum
biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the
terminal oxidation pathway for the n-alkane degradation, and the salicylate and phthalate pathways for
fluorene biodegradation. Polyextremophilic P. aeruginosa san ai, as a biosurfactant producer... with
multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental
bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or
elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals.
Keywords:
biodegradation / petroleum n-alkanes / polycyclic aromatic hydrocarbons / Pseudomonas aeruginosa / petroleum compounds / environmental bioremediationSource:
RSC Advances, 2020, 10, 24, 14060-14070Publisher:
- Royal Society of Chemistry
Funding / projects:
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Geochemical investigations of sedimentary rocks - fossil fuels and environmental pollutants (RS-MESTD-Basic Research (BR or ON)-176006)
DOI: 10.1039/c9ra10371f
ISSN: 20462069
WoS: 000528043700018
Scopus: 2-s2.0-85083442472
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IHTMTY - JOUR AU - Medić, Ana AU - Lješević, Marija AU - Inui, Hideyuku AU - Beškoski, Vladimir AU - Kojić, Ivan AU - Stojanović, Ksenija AU - Karadžić, Ivanka PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3576 AB - Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%, respectively, at initial concentrations of 20 mg L 1 and in seven days. P. aeruginosa san ai showed a high biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from crude oil. The efficiency of P. aeruginosa san ai degradation of crude oil fractions in seven days reached stage 3–4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the terminal oxidation pathway for the n-alkane degradation, and the salicylate and phthalate pathways for fluorene biodegradation. Polyextremophilic P. aeruginosa san ai, as a biosurfactant producer with multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals. PB - Royal Society of Chemistry T2 - RSC Advances T1 - Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity VL - 10 IS - 24 SP - 14060 EP - 14070 DO - 10.1039/c9ra10371f ER -
@article{ author = "Medić, Ana and Lješević, Marija and Inui, Hideyuku and Beškoski, Vladimir and Kojić, Ivan and Stojanović, Ksenija and Karadžić, Ivanka", year = "2020", abstract = "Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%, respectively, at initial concentrations of 20 mg L 1 and in seven days. P. aeruginosa san ai showed a high biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from crude oil. The efficiency of P. aeruginosa san ai degradation of crude oil fractions in seven days reached stage 3–4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the terminal oxidation pathway for the n-alkane degradation, and the salicylate and phthalate pathways for fluorene biodegradation. Polyextremophilic P. aeruginosa san ai, as a biosurfactant producer with multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals.", publisher = "Royal Society of Chemistry", journal = "RSC Advances", title = "Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity", volume = "10", number = "24", pages = "14060-14070", doi = "10.1039/c9ra10371f" }
Medić, A., Lješević, M., Inui, H., Beškoski, V., Kojić, I., Stojanović, K.,& Karadžić, I.. (2020). Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity. in RSC Advances Royal Society of Chemistry., 10(24), 14060-14070. https://doi.org/10.1039/c9ra10371f
Medić A, Lješević M, Inui H, Beškoski V, Kojić I, Stojanović K, Karadžić I. Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity. in RSC Advances. 2020;10(24):14060-14070. doi:10.1039/c9ra10371f .
Medić, Ana, Lješević, Marija, Inui, Hideyuku, Beškoski, Vladimir, Kojić, Ivan, Stojanović, Ksenija, Karadžić, Ivanka, "Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity" in RSC Advances, 10, no. 24 (2020):14060-14070, https://doi.org/10.1039/c9ra10371f . .