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Evaluation of assays for screening polycyclic aromatic hydrocarbon-degrading potential of bacteria

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2020
1451-93721900023L.pdf (517.3Kb)
Authors
Lješević, Marija
Milić, Jelena
Gojgić-Cvijović, Gordana
Šolević Knudsen, Tatjana
Ilić, Mila
Avdalović, Jelena
Vrvić, Miroslav
Article (Published version)
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Abstract
Within a 30-day incubation laboratory study, the polycyclic aromatic hydrocarbon (PAH) degradation profile of two bacteria, Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05 were studied by three microtiter plate assays to reveal the combination of certain biological and biochemical characteristics which are reliable indicators in evaluation of bacterial biodegradation abilities. The three assays, which are hydrocarbon growth assay, 2,6-DCPIP assay and dehydrogenase activity assay revealed that Rhodococcus sp. RNP05 exhibited better potential for PAH degradation than Planomicrobium sp. RNP01. Differences between initial and final optical density and specific growth rate constants were significantly higher (r = 0.995, P < 0.05) in case of Rhodococcus sp. RNP05 on all tested substrates, as compared to Planomicrobium sp. RNP01. This was confirmed by GC-FID analyses. Dehydrogenase activity of Rhodococcus sp. RNP05 was higher (r = 0.9995, P < 0.05) than Planomicrobium sp. RNP01 and correla...ted positively with the hydrocarbon growth assay (r = 0.999, P < 0.05, for Rhodococcus sp. RNP05, r = 0.986, P < 0.05 for Planomicrobium sp. RNP01). This study has shown that the combination of these assays could be used for determining the bioremediation potential of PAHs in petroleum contaminated soil with the ability of screening a large number of bacterial strains.

Degradacija policikličnih aromatičnih ugljovodonika (polycyclic aromatic hydrocarbons - PAH) ispitivana je testovima u mikrotitar pločama pomoću dva bakterijska soja Planomicrobium sp. RNP01 i Rhodococcus sp. RNP05, u periodu od 30 dana. Rezultati ispitivanja su ukazali da se kombinacijom određenih bioloških i biohemijskih karakteristika mogu kreirati dobri indikatori u proceni bakterijskog degradacionog potencijala. Tri testa, 2,6-DCPIP test, test dehidrogenazne aktivnosti i test rasta na ugljovodonicima pokazali su da Rhodococcus sp. RNP05 ima veći potencijal za degradaciju PAH jednjenja u odnosu na Planomicrobium sp. RNP01. Razlike u početnoj i krajnjoj optičkoj gustini i specifične konstante rasta bile su značajno više (r = 0,995, P < 0,05) u testu sa Rhodococcus sp. RNP05 na svim testiranim supstratima, u poređenju sa Planomicrobium sp. RNP01, a dobijeni rezultati su potvrđeni gasno-hromatogafskom-FID analizom. Dehidrogenazna aktivnost soja Rhodococcus sp. RNP05 bila je viša u odn...osu na Planomicrobium sp. RNP01 (r = 0,9995, P < 0,05) i u pozitivnoj korelaciji sa testom rasta na ugljovodonicima (r = 0,999, P < 0,05, za Rhodococcus sp. RNP05, r = 0,986, P < 0,05 za Planomicrobium sp. RNP01). Rezultati prikazanog istraživanja ukazuju na to da se kombinacija ovih testova može koristiti za određivanje bioremedijacionog potencijala za razgradnju PAH jedinjenja u zemljištu zagađenim naftom, pri čemu se dobija i mogućnost testiranja velikog broja bakterijskih sojeva.

Keywords:
hydrocarbon growth / PAHs / screening assays / Dehydrogenase activity / Planomicrobium / Rhodococcus / rast na ugljovodonicima / skrining testovi / dehidrogenazna aktivnost
Source:
Chemical Industry and Chemical Engineering Quarterly, 2020, 26, 1, 41-48
Publisher:
  • Savez hemijskih inženjera Srbije, Beograd
Funding / projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)

DOI: 10.2298/CICEQ190220023L

ISSN: 1451-9372; 2217-7434

WoS: 000528214600005

Scopus: 2-s2.0-85084000910
[ Google Scholar ]
3
1
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/3517
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Lješević, Marija
AU  - Milić, Jelena
AU  - Gojgić-Cvijović, Gordana
AU  - Šolević Knudsen, Tatjana
AU  - Ilić, Mila
AU  - Avdalović, Jelena
AU  - Vrvić, Miroslav
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3517
AB  - Within a 30-day incubation laboratory study, the polycyclic aromatic hydrocarbon (PAH) degradation profile of two bacteria, Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05 were studied by three microtiter plate assays to reveal the combination of certain biological and biochemical characteristics which are reliable indicators in evaluation of bacterial biodegradation abilities. The three assays, which are hydrocarbon growth assay, 2,6-DCPIP assay and dehydrogenase activity assay revealed that Rhodococcus sp. RNP05 exhibited better potential for PAH degradation than Planomicrobium sp. RNP01. Differences between initial and final optical density and specific growth rate constants were significantly higher (r = 0.995, P < 0.05) in case of Rhodococcus sp. RNP05 on all tested substrates, as compared to Planomicrobium sp. RNP01. This was confirmed by GC-FID analyses. Dehydrogenase activity of Rhodococcus sp. RNP05 was higher (r = 0.9995, P < 0.05) than Planomicrobium sp. RNP01 and correlated positively with the hydrocarbon growth assay (r = 0.999, P < 0.05, for Rhodococcus sp. RNP05, r = 0.986, P < 0.05 for Planomicrobium sp. RNP01). This study has shown that the combination of these assays could be used for determining the bioremediation potential of PAHs in petroleum contaminated soil with the ability of screening a large number of bacterial strains.
AB  - Degradacija policikličnih aromatičnih ugljovodonika (polycyclic aromatic hydrocarbons - PAH) ispitivana je testovima u mikrotitar pločama pomoću dva bakterijska soja Planomicrobium sp. RNP01 i Rhodococcus sp. RNP05, u periodu od 30 dana. Rezultati ispitivanja su ukazali da se kombinacijom određenih bioloških i biohemijskih karakteristika mogu kreirati dobri indikatori u proceni bakterijskog degradacionog potencijala. Tri testa, 2,6-DCPIP test, test dehidrogenazne aktivnosti i test rasta na ugljovodonicima pokazali su da Rhodococcus sp. RNP05 ima veći potencijal za degradaciju PAH jednjenja u odnosu na Planomicrobium sp. RNP01. Razlike u početnoj i krajnjoj optičkoj gustini i specifične konstante rasta bile su značajno više (r = 0,995, P < 0,05) u testu sa Rhodococcus sp. RNP05 na svim testiranim supstratima, u poređenju sa Planomicrobium sp. RNP01, a dobijeni rezultati su potvrđeni gasno-hromatogafskom-FID analizom. Dehidrogenazna aktivnost soja Rhodococcus sp. RNP05 bila je viša u odnosu na Planomicrobium sp. RNP01 (r = 0,9995, P < 0,05) i u pozitivnoj korelaciji sa testom rasta na ugljovodonicima (r = 0,999, P < 0,05, za Rhodococcus sp. RNP05, r = 0,986, P < 0,05 za Planomicrobium sp. RNP01). Rezultati prikazanog istraživanja ukazuju na to da se kombinacija ovih testova može koristiti za određivanje bioremedijacionog potencijala za razgradnju PAH jedinjenja u zemljištu zagađenim naftom, pri čemu se dobija i mogućnost testiranja velikog broja bakterijskih sojeva.
PB  - Savez hemijskih inženjera Srbije, Beograd
T2  - Chemical Industry and Chemical Engineering Quarterly
T1  - Evaluation of assays for screening polycyclic aromatic hydrocarbon-degrading potential of bacteria
VL  - 26
IS  - 1
SP  - 41
EP  - 48
DO  - 10.2298/CICEQ190220023L
ER  - 
@article{
author = "Lješević, Marija and Milić, Jelena and Gojgić-Cvijović, Gordana and Šolević Knudsen, Tatjana and Ilić, Mila and Avdalović, Jelena and Vrvić, Miroslav",
year = "2020",
abstract = "Within a 30-day incubation laboratory study, the polycyclic aromatic hydrocarbon (PAH) degradation profile of two bacteria, Planomicrobium sp. RNP01 and Rhodococcus sp. RNP05 were studied by three microtiter plate assays to reveal the combination of certain biological and biochemical characteristics which are reliable indicators in evaluation of bacterial biodegradation abilities. The three assays, which are hydrocarbon growth assay, 2,6-DCPIP assay and dehydrogenase activity assay revealed that Rhodococcus sp. RNP05 exhibited better potential for PAH degradation than Planomicrobium sp. RNP01. Differences between initial and final optical density and specific growth rate constants were significantly higher (r = 0.995, P < 0.05) in case of Rhodococcus sp. RNP05 on all tested substrates, as compared to Planomicrobium sp. RNP01. This was confirmed by GC-FID analyses. Dehydrogenase activity of Rhodococcus sp. RNP05 was higher (r = 0.9995, P < 0.05) than Planomicrobium sp. RNP01 and correlated positively with the hydrocarbon growth assay (r = 0.999, P < 0.05, for Rhodococcus sp. RNP05, r = 0.986, P < 0.05 for Planomicrobium sp. RNP01). This study has shown that the combination of these assays could be used for determining the bioremediation potential of PAHs in petroleum contaminated soil with the ability of screening a large number of bacterial strains., Degradacija policikličnih aromatičnih ugljovodonika (polycyclic aromatic hydrocarbons - PAH) ispitivana je testovima u mikrotitar pločama pomoću dva bakterijska soja Planomicrobium sp. RNP01 i Rhodococcus sp. RNP05, u periodu od 30 dana. Rezultati ispitivanja su ukazali da se kombinacijom određenih bioloških i biohemijskih karakteristika mogu kreirati dobri indikatori u proceni bakterijskog degradacionog potencijala. Tri testa, 2,6-DCPIP test, test dehidrogenazne aktivnosti i test rasta na ugljovodonicima pokazali su da Rhodococcus sp. RNP05 ima veći potencijal za degradaciju PAH jednjenja u odnosu na Planomicrobium sp. RNP01. Razlike u početnoj i krajnjoj optičkoj gustini i specifične konstante rasta bile su značajno više (r = 0,995, P < 0,05) u testu sa Rhodococcus sp. RNP05 na svim testiranim supstratima, u poređenju sa Planomicrobium sp. RNP01, a dobijeni rezultati su potvrđeni gasno-hromatogafskom-FID analizom. Dehidrogenazna aktivnost soja Rhodococcus sp. RNP05 bila je viša u odnosu na Planomicrobium sp. RNP01 (r = 0,9995, P < 0,05) i u pozitivnoj korelaciji sa testom rasta na ugljovodonicima (r = 0,999, P < 0,05, za Rhodococcus sp. RNP05, r = 0,986, P < 0,05 za Planomicrobium sp. RNP01). Rezultati prikazanog istraživanja ukazuju na to da se kombinacija ovih testova može koristiti za određivanje bioremedijacionog potencijala za razgradnju PAH jedinjenja u zemljištu zagađenim naftom, pri čemu se dobija i mogućnost testiranja velikog broja bakterijskih sojeva.",
publisher = "Savez hemijskih inženjera Srbije, Beograd",
journal = "Chemical Industry and Chemical Engineering Quarterly",
title = "Evaluation of assays for screening polycyclic aromatic hydrocarbon-degrading potential of bacteria",
volume = "26",
number = "1",
pages = "41-48",
doi = "10.2298/CICEQ190220023L"
}
Lješević, M., Milić, J., Gojgić-Cvijović, G., Šolević Knudsen, T., Ilić, M., Avdalović, J.,& Vrvić, M.. (2020). Evaluation of assays for screening polycyclic aromatic hydrocarbon-degrading potential of bacteria. in Chemical Industry and Chemical Engineering Quarterly
Savez hemijskih inženjera Srbije, Beograd., 26(1), 41-48.
https://doi.org/10.2298/CICEQ190220023L
Lješević M, Milić J, Gojgić-Cvijović G, Šolević Knudsen T, Ilić M, Avdalović J, Vrvić M. Evaluation of assays for screening polycyclic aromatic hydrocarbon-degrading potential of bacteria. in Chemical Industry and Chemical Engineering Quarterly. 2020;26(1):41-48.
doi:10.2298/CICEQ190220023L .
Lješević, Marija, Milić, Jelena, Gojgić-Cvijović, Gordana, Šolević Knudsen, Tatjana, Ilić, Mila, Avdalović, Jelena, Vrvić, Miroslav, "Evaluation of assays for screening polycyclic aromatic hydrocarbon-degrading potential of bacteria" in Chemical Industry and Chemical Engineering Quarterly, 26, no. 1 (2020):41-48,
https://doi.org/10.2298/CICEQ190220023L . .

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