Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor

Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to th...

Keywords:

Human serum albumin / Electrochemical biosensor / Immunosensor / Screen-printed carbon electrode

Source:
Journal of Electroanalytical Chemistry, 2020, 860, 113928-

Publisher:

Elsevier

Funding / projects:

Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring (RS-172030)
CEEPUS network: CIII-CZ-0212-12-1819-M-120151
EUREKA project E! 13303 MED-BIO-TEST

Note:

This is the peer-reviewed version of the article: Journal of Electroanalytical Chemistry, 2020, 860, 113928, doi: https://doi.org/10.1016/j.jelechem.2020.113928
The published version: http://cer.ihtm.bg.ac.rs/handle/123456789/3419

DOI: 10.1016/j.jelechem.2020.113928

ISSN: 1572-6657

WoS: 000519336100030

Scopus: 2-s2.0-85079132277

[ Google Scholar ]


	26
	15

TY  - JOUR
AU  - Stanković, Vesna
AU  - Đurđić, Slađana
AU  - Ognjanović, Miloš
AU  - Antić, Bratislav
AU  - Kalcher, Kurt
AU  - Mutić, Jelena
AU  - Stanković, Dalibor
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3420
AB  - Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice.
PB  - Elsevier
T2  - Journal of Electroanalytical Chemistry
T1  - Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor
VL  - 860
SP  - 113928
DO  - 10.1016/j.jelechem.2020.113928
ER  -

@article{
author = "Stanković, Vesna and Đurđić, Slađana and Ognjanović, Miloš and Antić, Bratislav and Kalcher, Kurt and Mutić, Jelena and Stanković, Dalibor",
year = "2020",
abstract = "Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice.",
publisher = "Elsevier",
journal = "Journal of Electroanalytical Chemistry",
title = "Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor",
volume = "860",
pages = "113928",
doi = "10.1016/j.jelechem.2020.113928"
}

Stanković, V., Đurđić, S., Ognjanović, M., Antić, B., Kalcher, K., Mutić, J.,& Stanković, D.. (2020). Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor. in Journal of Electroanalytical Chemistry
Elsevier., 860, 113928.
https://doi.org/10.1016/j.jelechem.2020.113928

Stanković V, Đurđić S, Ognjanović M, Antić B, Kalcher K, Mutić J, Stanković D. Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor. in Journal of Electroanalytical Chemistry. 2020;860:113928.
doi:10.1016/j.jelechem.2020.113928 .

Stanković, Vesna, Đurđić, Slađana, Ognjanović, Miloš, Antić, Bratislav, Kalcher, Kurt, Mutić, Jelena, Stanković, Dalibor, "Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor" in Journal of Electroanalytical Chemistry, 860 (2020):113928,
https://doi.org/10.1016/j.jelechem.2020.113928 . .