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dc.creatorStanković, Vesna
dc.creatorĐurđić, Slađana
dc.creatorOgnjanović, Miloš
dc.creatorAntić, Bratislav
dc.creatorKalcher, Kurt
dc.creatorMutić, Jelena
dc.creatorStanković, Dalibor
dc.date.accessioned2020-02-24T10:06:26Z
dc.date.available2020-02-24T10:06:26Z
dc.date.issued2020
dc.identifier.issn15726657
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3419
dc.description.abstractDiagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice.en
dc.publisherElsevier BVen
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172030/RS//en
dc.relationCEEPUS network: CIII-CZ-0212-12-1819-M-120151en
dc.relationEUREKA project E! 13303 MED-BIO-TESTen
dc.rightsrestrictedAccess
dc.sourceJournal of Electroanalytical Chemistryen
dc.subjectHuman serum albumin
dc.subjectElectrochemical biosensor
dc.subjectImmunosensor
dc.subjectScreen-printed carbon electrode
dc.titleAnti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensoren
dc.typearticleen
dc.rights.licenseARRen
dcterms.abstractЂурђић, Слађана; Огњановић, Милош; Станковић, Весна; Aнтић, Братислав; Калцхер, Курт; Мутић, Јелена; Станковић, Далибор;
dc.rights.holderElsevier
dc.citation.volume860
dc.citation.spage113928
dc.citation.rankM21~
dc.description.otherThe peer-reviewed version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3420]
dc.identifier.doi10.1016/j.jelechem.2020.113928
dc.identifier.scopus2-s2.0-85079132277
dc.identifier.wos000519336100030
dc.type.versionpublishedVersion


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