Characterization of recombinant antibodies for detection of TNT and its derivatives
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Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.
Keywords:TNT / assay / antibody / TNP–Tris / recombinant / detection
Source:Chemical Papers, 2009, 63, 4, 391-398
- Ministry of Science