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Characterization of recombinant antibodies for detection of TNT and its derivatives

Authorized Users Only
2009
Authors
Simonović, Mladen
Milošević-Zlatanović, Svetlana
Milosavić, Nenad
Vrvić, Miroslav
Simonović, Branislav
Article (Published version)
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Abstract
Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.
Keywords:
TNT / assay / antibody / TNP–Tris / recombinant / detection
Source:
Chemical Papers, 2009, 63, 4, 391-398
Publisher:
  • Versita
Funding / projects:
  • Ministry of Science

DOI: 10.2478/s11696-009-0043-5

ISSN: 0366-6352; 1336-9075

WoS: 000266447300003

Scopus: 2-s2.0-67650616228
[ Google Scholar ]
4
4
URI
https://cer.ihtm.bg.ac.rs/handle/123456789/3414
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - JOUR
AU  - Simonović, Mladen
AU  - Milošević-Zlatanović, Svetlana
AU  - Milosavić, Nenad
AU  - Vrvić, Miroslav
AU  - Simonović, Branislav
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3414
AB  - Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.
PB  - Versita
T2  - Chemical Papers
T1  - Characterization of recombinant antibodies for detection of TNT and its derivatives
VL  - 63
IS  - 4
SP  - 391
EP  - 398
DO  - 10.2478/s11696-009-0043-5
ER  - 
@article{
author = "Simonović, Mladen and Milošević-Zlatanović, Svetlana and Milosavić, Nenad and Vrvić, Miroslav and Simonović, Branislav",
year = "2009",
abstract = "Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.",
publisher = "Versita",
journal = "Chemical Papers",
title = "Characterization of recombinant antibodies for detection of TNT and its derivatives",
volume = "63",
number = "4",
pages = "391-398",
doi = "10.2478/s11696-009-0043-5"
}
Simonović, M., Milošević-Zlatanović, S., Milosavić, N., Vrvić, M.,& Simonović, B.. (2009). Characterization of recombinant antibodies for detection of TNT and its derivatives. in Chemical Papers
Versita., 63(4), 391-398.
https://doi.org/10.2478/s11696-009-0043-5
Simonović M, Milošević-Zlatanović S, Milosavić N, Vrvić M, Simonović B. Characterization of recombinant antibodies for detection of TNT and its derivatives. in Chemical Papers. 2009;63(4):391-398.
doi:10.2478/s11696-009-0043-5 .
Simonović, Mladen, Milošević-Zlatanović, Svetlana, Milosavić, Nenad, Vrvić, Miroslav, Simonović, Branislav, "Characterization of recombinant antibodies for detection of TNT and its derivatives" in Chemical Papers, 63, no. 4 (2009):391-398,
https://doi.org/10.2478/s11696-009-0043-5 . .

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