Characterization of recombinant antibodies for detection of TNT and its derivatives
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2009
Authors
Simonović, Mladen
Milošević-Zlatanović, Svetlana

Milosavić, Nenad
Vrvić, Miroslav

Simonović, Branislav

Article (Published version)

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Show full item recordAbstract
Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.
Keywords:
TNT / assay / antibody / TNP–Tris / recombinant / detectionSource:
Chemical Papers, 2009, 63, 4, 391-398Publisher:
- Versita
Funding / projects:
- Ministry of Science
DOI: 10.2478/s11696-009-0043-5
ISSN: 0366-6352; 1336-9075
WoS: 000266447300003
Scopus: 2-s2.0-67650616228
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IHTMTY - JOUR AU - Simonović, Mladen AU - Milošević-Zlatanović, Svetlana AU - Milosavić, Nenad AU - Vrvić, Miroslav AU - Simonović, Branislav PY - 2009 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3414 AB - Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies. PB - Versita T2 - Chemical Papers T1 - Characterization of recombinant antibodies for detection of TNT and its derivatives VL - 63 IS - 4 SP - 391 EP - 398 DO - 10.2478/s11696-009-0043-5 ER -
@article{ author = "Simonović, Mladen and Milošević-Zlatanović, Svetlana and Milosavić, Nenad and Vrvić, Miroslav and Simonović, Branislav", year = "2009", abstract = "Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.", publisher = "Versita", journal = "Chemical Papers", title = "Characterization of recombinant antibodies for detection of TNT and its derivatives", volume = "63", number = "4", pages = "391-398", doi = "10.2478/s11696-009-0043-5" }
Simonović, M., Milošević-Zlatanović, S., Milosavić, N., Vrvić, M.,& Simonović, B.. (2009). Characterization of recombinant antibodies for detection of TNT and its derivatives. in Chemical Papers Versita., 63(4), 391-398. https://doi.org/10.2478/s11696-009-0043-5
Simonović M, Milošević-Zlatanović S, Milosavić N, Vrvić M, Simonović B. Characterization of recombinant antibodies for detection of TNT and its derivatives. in Chemical Papers. 2009;63(4):391-398. doi:10.2478/s11696-009-0043-5 .
Simonović, Mladen, Milošević-Zlatanović, Svetlana, Milosavić, Nenad, Vrvić, Miroslav, Simonović, Branislav, "Characterization of recombinant antibodies for detection of TNT and its derivatives" in Chemical Papers, 63, no. 4 (2009):391-398, https://doi.org/10.2478/s11696-009-0043-5 . .