In the present investigation the antiproliferative
activity of thirteen derivatives of betulinic acid and
betulin was tested against five different tumor cell lines.
The toxicity against normal human fibroblasts
(WWO70327) and the mode of cell death on HT-29
(colon cancer) as well as caspase activity induced by the
most active compounds, 9 (3-O-chloroacetylbetulinic
acid) and 15 (28-O-chloroacetylbetulin) were determined.
Investigated derivatives exerted a dose dependent antiproliferative
action at micromolar concentrations toward
target tumor cell lines. Treatment of HT-29 cells for 24 h
with 9 and 15 induced apoptosis, as observed by dye
exclusion test (trypan blue) and confirmed by the
appearance of a typical ladder pattern in the DNA
fragmentation assay.
The authors are grateful to the Wirtschaftsministerium Sachsen-Anhalt for financial support (Antitumor drugs on the basis of natural compounds FuE 62/05).
TY - JOUR
AU - Kommera, Harish
AU - Kaluđerović, Goran N.
AU - Kalbitz, Jutta
AU - Paschke, Reinhard
PY - 2011
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3411
AB - In the present investigation the antiproliferative
activity of thirteen derivatives of betulinic acid and
betulin was tested against five different tumor cell lines.
The toxicity against normal human fibroblasts
(WWO70327) and the mode of cell death on HT-29
(colon cancer) as well as caspase activity induced by the
most active compounds, 9 (3-O-chloroacetylbetulinic
acid) and 15 (28-O-chloroacetylbetulin) were determined.
Investigated derivatives exerted a dose dependent antiproliferative
action at micromolar concentrations toward
target tumor cell lines. Treatment of HT-29 cells for 24 h
with 9 and 15 induced apoptosis, as observed by dye
exclusion test (trypan blue) and confirmed by the
appearance of a typical ladder pattern in the DNA
fragmentation assay.
PB - Netherlands : Springer
T2 - Investigational New Drugs
T1 - Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells
VL - 29
IS - 2
SP - 266
EP - 272
DO - 10.1007/s10637-009-9358-x
ER -
@article{
author = "Kommera, Harish and Kaluđerović, Goran N. and Kalbitz, Jutta and Paschke, Reinhard",
year = "2011",
abstract = "In the present investigation the antiproliferative
activity of thirteen derivatives of betulinic acid and
betulin was tested against five different tumor cell lines.
The toxicity against normal human fibroblasts
(WWO70327) and the mode of cell death on HT-29
(colon cancer) as well as caspase activity induced by the
most active compounds, 9 (3-O-chloroacetylbetulinic
acid) and 15 (28-O-chloroacetylbetulin) were determined.
Investigated derivatives exerted a dose dependent antiproliferative
action at micromolar concentrations toward
target tumor cell lines. Treatment of HT-29 cells for 24 h
with 9 and 15 induced apoptosis, as observed by dye
exclusion test (trypan blue) and confirmed by the
appearance of a typical ladder pattern in the DNA
fragmentation assay.",
publisher = "Netherlands : Springer",
journal = "Investigational New Drugs",
title = "Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells",
volume = "29",
number = "2",
pages = "266-272",
doi = "10.1007/s10637-009-9358-x"
}
Kommera, H., Kaluđerović, G. N., Kalbitz, J.,& Paschke, R.. (2011). Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells. in Investigational New Drugs
Netherlands : Springer., 29(2), 266-272.
https://doi.org/10.1007/s10637-009-9358-x
Kommera H, Kaluđerović GN, Kalbitz J, Paschke R. Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells. in Investigational New Drugs. 2011;29(2):266-272.
doi:10.1007/s10637-009-9358-x .
Kommera, Harish, Kaluđerović, Goran N., Kalbitz, Jutta, Paschke, Reinhard, "Lupane Triterpenoids—Betulin and Betulinic acid derivatives induce apoptosis in tumor cells" in Investigational New Drugs, 29, no. 2 (2011):266-272,
https://doi.org/10.1007/s10637-009-9358-x . .
