pH-dependent solubility profile of Desipramine hydrochloride (Ds.HCl) was studied using pH-ramp shake flask method. First, the pH value of DsHCl stock solution in 0.15 M phosphate buffer was adjusted to 11.7 in order to minimize supersaturation effect. Then, the pH value in separate samples was adjusted downwards with HCl, to prepare solutions in the pH 1.7-11.7 region. After stirring (6 h) and sedimentation (18 h), PTFE (hydrophobic, pore size 0.22 µm) filters or centrifugation were used for phase separation. Concentration was measured using HPLC with UV/Vis detection. The computer program pDISOL-X was used for data processing and refinement of equilibrium constants. Different techniques were used for solid phase characterization.
TY - CONF
AU - Marković, Olivera
AU - Pešić, Miloš P.
AU - Verbić, Tatjana
AU - Avdeef, Alex
PY - 2017
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/3402
AB - pH-dependent solubility profile of Desipramine hydrochloride (Ds.HCl) was studied using pH-ramp shake flask method. First, the pH value of DsHCl stock solution in 0.15 M phosphate buffer was adjusted to 11.7 in order to minimize supersaturation effect. Then, the pH value in separate samples was adjusted downwards with HCl, to prepare solutions in the pH 1.7-11.7 region. After stirring (6 h) and sedimentation (18 h), PTFE (hydrophobic, pore size 0.22 µm) filters or centrifugation were used for phase separation. Concentration was measured using HPLC with UV/Vis detection. The computer program pDISOL-X was used for data processing and refinement of equilibrium constants. Different techniques were used for solid phase characterization.
PB - International Association of Physical Chemists
C3 - 6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts
T1 - pH-Dependent solubility profile of desipramine hydrochloride
SP - 42
EP - 42
ER -
@conference{
author = "Marković, Olivera and Pešić, Miloš P. and Verbić, Tatjana and Avdeef, Alex",
year = "2017",
url = "http://cer.ihtm.bg.ac.rs/handle/123456789/3402",
abstract = "pH-dependent solubility profile of Desipramine hydrochloride (Ds.HCl) was studied using pH-ramp shake flask method. First, the pH value of DsHCl stock solution in 0.15 M phosphate buffer was adjusted to 11.7 in order to minimize supersaturation effect. Then, the pH value in separate samples was adjusted downwards with HCl, to prepare solutions in the pH 1.7-11.7 region. After stirring (6 h) and sedimentation (18 h), PTFE (hydrophobic, pore size 0.22 µm) filters or centrifugation were used for phase separation. Concentration was measured using HPLC with UV/Vis detection. The computer program pDISOL-X was used for data processing and refinement of equilibrium constants. Different techniques were used for solid phase characterization.",
publisher = "International Association of Physical Chemists",
journal = "6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts",
title = "pH-Dependent solubility profile of desipramine hydrochloride",
pages = "42-42"
}
Marković, O., Pešić, M. P., Verbić, T.,& Avdeef, A. (2017). pH-Dependent solubility profile of desipramine hydrochloride.
6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts
International Association of Physical Chemists., 42-42.
Marković O, Pešić MP, Verbić T, Avdeef A. pH-Dependent solubility profile of desipramine hydrochloride. 6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts. 2017;:42-42
Marković Olivera, Pešić Miloš P., Verbić Tatjana, Avdeef Alex, "pH-Dependent solubility profile of desipramine hydrochloride" 6th World Conference on Physico-Chemical Methods in Drug Discovery Zagreb, Croatia, September 4-7, 2017 - Book of Abstracts (2017):42-42
