Prikaz osnovnih podataka o dokumentu
Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain
Fina strukturna analiza fungalnog polisaharida pululana dobijenog pomoću Aureobasidium pullulans, soj CH-1
dc.creator | Jakovljević, Dragica | |
dc.creator | Vrvić, Miroslav | |
dc.creator | Radulović, Milanka | |
dc.creator | Hranisavljević-Jakovljević, Mirjana | |
dc.date.accessioned | 2019-01-30T17:09:33Z | |
dc.date.available | 2019-01-30T17:09:33Z | |
dc.date.issued | 2001 | |
dc.identifier.issn | 0352-5139 | |
dc.identifier.uri | https://cer.ihtm.bg.ac.rs/handle/123456789/33 | |
dc.description.abstract | The structure of pullulan, the extracellular α-D-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a linear α-D-glucan consisting of maltotriosyl repeat units connected terminally by (1->6)-α-D-glucosidic bonds. Occasionally some of maltotriosyl residues are replaced by higher oligosaccharide units, most frequently with maltotetraosyl residues. Using the susceptibility of pullulan CH-1 (obtained from strain CH-1 of Aureobasidium pullulans) to hydrolysis catalysed by porcine alpha-amylase, the polysaccharide was cleaved and the fragments obtained fractionated by gel-permeation chromatography. The heterogenous size of the fragments indicates that there is no apparent regular distribution of tetrasaccharide units in the pullulan chain. Enzymatic digestion of pullulan CH-1 using pullulanase, followed by gel-permeation chromatography of the resulting digest confirmed these results as did preparative paper chromatography and CI mass spectrometry of the separated components, i.e., that maltotetraosyl units (about 7 %) are building units of pullulan CH-1. | en |
dc.description.abstract | Pululan, ekstracelularni polisaharid koji proizvodi kvascu slična gljiva Aureobasidium pullulans, jeste linearni glukan čiju strukturu pretežno čine polimaltotriozil ostatci povezani međusobno α-(1->6)-glikozidnim vezama. Varijacije u strukturi ovog polisaharida mogu nastati usled zamene maltotrioznih ostataka na pojedinim mestima polimernog lanca oligosaharidnim fragmentima dužeg niza, najčešće maltotetraoznim ostacima. U ovom radu ispitan je pululan koji proizvodi A. pullulans, soj CH-1, enzimskom hidrolizom sa pankreasnom α-amilazom i pululanazom, sa ciljem da se utvrdi da li i u kom odnosu ovaj polisaharid sadrži oligosaharidne fragmente, kao i da se odredi dužina oligosaharidnog niza, odnosno broj monosaharidnih ostataka koji ulaze u njegov sastav. Primenom uobičajenih analitičkih metoda (gel-filtraciona hromatografija, metilaciona analiza, preparativna hromatografija kombinovana sa masenom spektrometrijom metilovanih oligosaharidnih alditola), utvrđeno je da su maltotetraozne jedinice (oko 7 %) integralni deo polisaharidnog lanca pululana CH-1. Raspored ovih jedinica je nasumičan. | sr |
dc.publisher | Serbian Chemical Society | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of the Serbian Chemical Society | |
dc.subject | Aureobasidium pullulans | en |
dc.subject | polysaccharide | en |
dc.subject | pullulan | en |
dc.subject | amylolysis | en |
dc.subject | pullulanolysis | en |
dc.title | Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain | en |
dc.title | Fina strukturna analiza fungalnog polisaharida pululana dobijenog pomoću Aureobasidium pullulans, soj CH-1 | sr |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Јаковљевић, Драгица; Врвић, Мирослав М.; Радуловић, Миланка; Хранисављевић-Јаковљевић, Мирјана; Фина структурна анализа фунгалног полисахарида пулулана добијеног помоћу Aуреобасидиум пуллуланс, сој ЦХ-1; Фина структурна анализа фунгалног полисахарида пулулана добијеног помоћу Aуреобасидиум пуллуланс, сој ЦХ-1; | |
dc.citation.volume | 66 | |
dc.citation.issue | 6 | |
dc.citation.spage | 377 | |
dc.citation.epage | 383 | |
dc.citation.other | 66(6): 377-383 | |
dc.citation.rank | M23 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_cer_33 | |
dc.identifier.fulltext | https://cer.ihtm.bg.ac.rs//bitstream/id/9000/31.pdf | |
dc.type.version | publishedVersion |