Show simple item record

Fina strukturna analiza fungalnog polisaharida pululana dobijenog pomoću Aureobasidium pullulans, soj CH-1

dc.creatorJakovljević, Dragica
dc.creatorVrvić, Miroslav
dc.creatorRadulović, Milanka
dc.creatorHranisavljević-Jakovljević, Mirjana
dc.date.accessioned2019-01-30T17:09:33Z
dc.date.available2019-01-30T17:09:33Z
dc.date.issued2001
dc.identifier.issn0352-5139
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/33
dc.description.abstractThe structure of pullulan, the extracellular α-D-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a linear α-D-glucan consisting of maltotriosyl repeat units connected terminally by (1->6)-α-D-glucosidic bonds. Occasionally some of maltotriosyl residues are replaced by higher oligosaccharide units, most frequently with maltotetraosyl residues. Using the susceptibility of pullulan CH-1 (obtained from strain CH-1 of Aureobasidium pullulans) to hydrolysis catalysed by porcine alpha-amylase, the polysaccharide was cleaved and the fragments obtained fractionated by gel-permeation chromatography. The heterogenous size of the fragments indicates that there is no apparent regular distribution of tetrasaccharide units in the pullulan chain. Enzymatic digestion of pullulan CH-1 using pullulanase, followed by gel-permeation chromatography of the resulting digest confirmed these results as did preparative paper chromatography and CI mass spectrometry of the separated components, i.e., that maltotetraosyl units (about 7 %) are building units of pullulan CH-1.en
dc.description.abstractPululan, ekstracelularni polisaharid koji proizvodi kvascu slična gljiva Aureobasidium pullulans, jeste linearni glukan čiju strukturu pretežno čine polimaltotriozil ostatci povezani međusobno α-(1->6)-glikozidnim vezama. Varijacije u strukturi ovog polisaharida mogu nastati usled zamene maltotrioznih ostataka na pojedinim mestima polimernog lanca oligosaharidnim fragmentima dužeg niza, najčešće maltotetraoznim ostacima. U ovom radu ispitan je pululan koji proizvodi A. pullulans, soj CH-1, enzimskom hidrolizom sa pankreasnom α-amilazom i pululanazom, sa ciljem da se utvrdi da li i u kom odnosu ovaj polisaharid sadrži oligosaharidne fragmente, kao i da se odredi dužina oligosaharidnog niza, odnosno broj monosaharidnih ostataka koji ulaze u njegov sastav. Primenom uobičajenih analitičkih metoda (gel-filtraciona hromatografija, metilaciona analiza, preparativna hromatografija kombinovana sa masenom spektrometrijom metilovanih oligosaharidnih alditola), utvrđeno je da su maltotetraozne jedinice (oko 7 %) integralni deo polisaharidnog lanca pululana CH-1. Raspored ovih jedinica je nasumičan.sr
dc.publisherSerbian Chemical Society
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceJournal of the Serbian Chemical Society
dc.subjectAureobasidium pullulansen
dc.subjectpolysaccharideen
dc.subjectpullulanen
dc.subjectamylolysisen
dc.subjectpullulanolysisen
dc.titleFine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strainen
dc.titleFina strukturna analiza fungalnog polisaharida pululana dobijenog pomoću Aureobasidium pullulans, soj CH-1sr
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractЈаковљевић, Драгица; Врвић, Мирослав М.; Радуловић, Миланка; Хранисављевић-Јаковљевић, Мирјана; Фина структурна анализа фунгалног полисахарида пулулана добијеног помоћу Aуреобасидиум пуллуланс, сој ЦХ-1; Фина структурна анализа фунгалног полисахарида пулулана добијеног помоћу Aуреобасидиум пуллуланс, сој ЦХ-1;
dc.citation.volume66
dc.citation.issue6
dc.citation.spage377
dc.citation.epage383
dc.citation.other66(6): 377-383
dc.citation.rankM23
dc.identifier.rcubConv_146
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs//bitstream/id/9000/31.pdf
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record