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dc.creatorSavić, Aleksandar
dc.creatorGligorijević, Nevenka
dc.creatorAranđelović, Sandra
dc.creatorDojčinović, Biljana
dc.creatorKaczmarek, Anna M.
dc.creatorRadulović, Siniša
dc.creatorVan Deun, Rik
dc.creatorVan Hecke, Kristof
dc.date.accessioned2020-01-20T21:23:12Z
dc.date.available2020-01-20T21:23:12Z
dc.date.issued2020
dc.identifier.issn0162-0134
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3352
dc.description.abstractThe monocationic chloro complexes containing chelating N∩N ligands: [(η6-p-cymene)Ru(L1–4)Cl]+ (1–4), where L1 = 4-methyl-1,10-phenantroline, L2 = dipyrido[3,2-a:2′,3′-c]phenazine, L3 = 11-chloro-dipyrido[3,2-a:2′,3′-c]phenazine, L4 = 11-nitro-dipyrido[3,2-a:2′,3′-c]phenazine; p-cymene = 1-methyl-4-isopropylbenzene) have been prepared and characterized as the hexafluorophosphate salts. The biological activity of 1–4 has been investigated in selected 2D monolayer cell cultures (A549, PANC-1, MDA-MB-231, MRC-5). All investigated ruthenium complexes showed similar or even better cytotoxicity to cisplatin. However, there was no significant reduction in growth of PANC-1 cells in a 3D cell culture of multicellular tumor spheroids (MCTS) after treatment with 2–4, while the cisplatin treatment induced retardation in MCTS growth. Flow cytometry analysis of the cell cycle of PANC-1 cells shows that 3 caused changes of cell cycle phase distribution characterized by slight accumulation of cells in the G2-M phase. Absence of the Sub-G1 phase in the cell cycle of the treated cells indicated that there was no fragmentation of DNA for the analyzed time intervals (48 and 72 h treatment). Fluorescent microscopy, after acridine orange/ethidium bromide staining, revealed that the investigated ruthenium complexes induced some characteristics of apoptotic morphology (shrinking and condensation of chromatin) with notably preserved integrity of the plasma membrane. Investigation of cellular uptake and DNA - fraction accumulation performed by inductively coupled plasma mass spectrometry in PANC-1 cells with equimolar concentrations (5 μM) of 2–4 and cisplatin showed more efficient cellular uptake and DNA - fraction accumulation of complex 3 compared to complexes 2 and 4.en
dc.publisherElsevier
dc.relationErasmus Mundus Basileus V project
dc.relationHercules Foundation (project AUGE/11/029 “3D-SPACE: 3D Structural Platform Aiming for Chemical Excellence”)
dc.relationSpecial Research Fund (BOF) – UGent (project 01N03217)
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172035/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41026/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Inorganic Biochemistry
dc.subject3D cell culture
dc.subjectCrystal structure
dc.subjectCytotoxicity
dc.subjectOrganoruthenium complexes
dc.titleAntitumor activity of organoruthenium complexes with chelate aromatic ligands, derived from 1,10-phenantroline: Synthesis and biological activityen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВан Деун, Рик; Дојчиновић, Биљана; Радуловић, Синиша; Глигоријевић, Невенка; Савић, Aлександар; Ван Хецке, Кристоф; Кацзмарек, Aнна М.; Aранђеловић, Сандра;
dc.citation.volume202
dc.citation.spage110869
dc.citation.spage110869
dc.citation.rankM21~
dc.identifier.doi10.1016/j.jinorgbio.2019.110869
dc.identifier.scopus2-s2.0-85073921098
dc.identifier.wos000502686300007
dc.type.versionpublishedVersion


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