Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ
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2020
Authors
Gavrilović, Branka R.
Prokić, Marko D.

Petrović, Tamara G.

Despotović, Svetlana G.

Radovanović, Tijana B.

Krizmanić, Imre I.

Ćirić, Miloš

Gavrić, Jelena P.

Article (Published version)

Elsevier
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There is little information in scientific literature as to how conditions created by a microcystin (MC) producing cyanobacterial bloom affect the oxidant/antioxidant, biotransformation and neurotoxicity parameters in adult frogs in situ. We investigated biochemical parameters in the skin and muscle of Pelophylax kl. esculentus from Lake Ludaš (Serbia) by comparing frogs that live on the northern bloom side (BS) of the lake with those that inhabit the southern no-bloom side (NBS). A higher protein carbonylation level and lower antioxidant defense system capability in the skin of frogs living in conditions of the cyanobacterial bloom were observed. Inhibition of glutathione-dependent machinery was the major mechanism responsible for the induction of cyanobacterial bloom-mediated oxidative stress in frog skin. On the other hand, the detected higher ability of muscle to overcome bloom prooxidant toxicity was linked to a higher efficiency of the biotransformation system through glutathione-...S-transferase activity and/or was the consequence of indirect exposure of the tissue to the bloom. Our results have also revealed that the cyanobacterial bloom conditions induced the cholinergic neurotransmitter system in both tissues. This study provides a better understanding of the ecotoxicological impact of the MC producing cyanobacterial bloom on frogs in situ. However, further investigations of the complex mechanism involved in cyanobacterial bloom toxicity in real environmental conditions are required.
Keywords:
Oxidative stress / Biotransformation / Neurotoxicity / Frog / MicrocystinSource:
Aquatic Toxicology, 2020, 220, 105399-Publisher:
- Elsevier
Funding / projects:
DOI: 10.1016/j.aquatox.2019.105399
ISSN: 0166-445X
WoS: 000517853700002
Scopus: 2-s2.0-85077023123
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IHTMTY - JOUR AU - Gavrilović, Branka R. AU - Prokić, Marko D. AU - Petrović, Tamara G. AU - Despotović, Svetlana G. AU - Radovanović, Tijana B. AU - Krizmanić, Imre I. AU - Ćirić, Miloš AU - Gavrić, Jelena P. PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3343 AB - There is little information in scientific literature as to how conditions created by a microcystin (MC) producing cyanobacterial bloom affect the oxidant/antioxidant, biotransformation and neurotoxicity parameters in adult frogs in situ. We investigated biochemical parameters in the skin and muscle of Pelophylax kl. esculentus from Lake Ludaš (Serbia) by comparing frogs that live on the northern bloom side (BS) of the lake with those that inhabit the southern no-bloom side (NBS). A higher protein carbonylation level and lower antioxidant defense system capability in the skin of frogs living in conditions of the cyanobacterial bloom were observed. Inhibition of glutathione-dependent machinery was the major mechanism responsible for the induction of cyanobacterial bloom-mediated oxidative stress in frog skin. On the other hand, the detected higher ability of muscle to overcome bloom prooxidant toxicity was linked to a higher efficiency of the biotransformation system through glutathione-S-transferase activity and/or was the consequence of indirect exposure of the tissue to the bloom. Our results have also revealed that the cyanobacterial bloom conditions induced the cholinergic neurotransmitter system in both tissues. This study provides a better understanding of the ecotoxicological impact of the MC producing cyanobacterial bloom on frogs in situ. However, further investigations of the complex mechanism involved in cyanobacterial bloom toxicity in real environmental conditions are required. PB - Elsevier T2 - Aquatic Toxicology T1 - Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ VL - 220 SP - 105399 DO - 10.1016/j.aquatox.2019.105399 ER -
@article{ author = "Gavrilović, Branka R. and Prokić, Marko D. and Petrović, Tamara G. and Despotović, Svetlana G. and Radovanović, Tijana B. and Krizmanić, Imre I. and Ćirić, Miloš and Gavrić, Jelena P.", year = "2020", abstract = "There is little information in scientific literature as to how conditions created by a microcystin (MC) producing cyanobacterial bloom affect the oxidant/antioxidant, biotransformation and neurotoxicity parameters in adult frogs in situ. We investigated biochemical parameters in the skin and muscle of Pelophylax kl. esculentus from Lake Ludaš (Serbia) by comparing frogs that live on the northern bloom side (BS) of the lake with those that inhabit the southern no-bloom side (NBS). A higher protein carbonylation level and lower antioxidant defense system capability in the skin of frogs living in conditions of the cyanobacterial bloom were observed. Inhibition of glutathione-dependent machinery was the major mechanism responsible for the induction of cyanobacterial bloom-mediated oxidative stress in frog skin. On the other hand, the detected higher ability of muscle to overcome bloom prooxidant toxicity was linked to a higher efficiency of the biotransformation system through glutathione-S-transferase activity and/or was the consequence of indirect exposure of the tissue to the bloom. Our results have also revealed that the cyanobacterial bloom conditions induced the cholinergic neurotransmitter system in both tissues. This study provides a better understanding of the ecotoxicological impact of the MC producing cyanobacterial bloom on frogs in situ. However, further investigations of the complex mechanism involved in cyanobacterial bloom toxicity in real environmental conditions are required.", publisher = "Elsevier", journal = "Aquatic Toxicology", title = "Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ", volume = "220", pages = "105399", doi = "10.1016/j.aquatox.2019.105399" }
Gavrilović, B. R., Prokić, M. D., Petrović, T. G., Despotović, S. G., Radovanović, T. B., Krizmanić, I. I., Ćirić, M.,& Gavrić, J. P.. (2020). Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ. in Aquatic Toxicology Elsevier., 220, 105399. https://doi.org/10.1016/j.aquatox.2019.105399
Gavrilović BR, Prokić MD, Petrović TG, Despotović SG, Radovanović TB, Krizmanić II, Ćirić M, Gavrić JP. Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ. in Aquatic Toxicology. 2020;220:105399. doi:10.1016/j.aquatox.2019.105399 .
Gavrilović, Branka R., Prokić, Marko D., Petrović, Tamara G., Despotović, Svetlana G., Radovanović, Tijana B., Krizmanić, Imre I., Ćirić, Miloš, Gavrić, Jelena P., "Biochemical parameters in skin and muscle of Pelophylax kl. esculentus frogs: Influence of a cyanobacterial bloom in situ" in Aquatic Toxicology, 220 (2020):105399, https://doi.org/10.1016/j.aquatox.2019.105399 . .