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dc.creatorProdić, I.
dc.creatorStanić-Vučinić, Dragana
dc.creatorApostolovic, D.
dc.creatorMihailović, Jelena
dc.creatorRadibratović, Milica
dc.creatorRadosavljevic, J.
dc.creatorBurazer, L.
dc.creatorMilčić, Miloš
dc.creatorSmiljanic, K.
dc.creatorvan, Hage M.
dc.creatorĆirković Veličković, Tanja
dc.date.accessioned2020-01-14T16:27:08Z
dc.date.available2019-01-09
dc.date.issued2018
dc.identifier.issn0954-7894
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3340
dc.description.abstractBackgroundMost food allergens sensitizing via the gastrointestinal tract are stable proteins that are resistant to pepsin digestion, in particular major peanut allergens, Ara h 2 and Ara h 6. Survival of their large fragments is essential for sensitizing capacity. However, the immunoreactive proteins/peptides to which the immune system of the gastrointestinal tract is exposed during digestion of peanut proteins are unknown. Particularly, the IgE reactivity of short digestion-resistant peptides (SDRPs; LT 10kDa) released by gastric digestion under standardized and physiologically relevant invitro conditions has not been investigated. ObjectiveThe aim of this study was to investigate and identify digestion products of major peanut allergens and in particular to examine IgE reactivity of SDRPs released by pepsin digestion of whole peanut grains. MethodsTwo-dimensional gel-based proteomics and shotgun peptidomics, immunoblotting with allergen-specific antibodies from peanut-sensitized patients, enzyme-linked immunosorbent inhibition assay and ImmunoCAP tests, including far ultraviolet-circular dichroism spectroscopy were used to identify and characterize peanut digesta. ResultsAra h 2 and Ara h 6 remained mostly intact, and SDRPs from Ara h 2 were more potent in inhibiting IgE binding than Ara h 1 and Ara 3. Ara h 1 and Ara h 3 exhibited sequential digestion into a series of digestion-resistant peptides with preserved allergenic capacity. A high number of identified SDRPs from Ara h 1, Ara h 2 and Ara h 3 were part of short continuous epitope sequences and possessed substantial allergenic potential. Conclusion and Clinical RelevancePeanut grain digestion by oral and gastric phase enzymes generates mixture of products, where the major peanut allergens remain intact and their digested peptides have preserved allergenic capacity highlighting their important roles in allergic reactions to peanut.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172024/RS//
dc.rightsembargoedAccess
dc.sourceClinical and Experimental Allergy
dc.subjectdigestion-resistant peptidesen
dc.subjectfood matrixen
dc.subjectgastric-simulated digestionen
dc.subjectpeanut allergyen
dc.subjectproteolysis resistanceen
dc.titleInfluence of peanut matrix on stability of allergens in gastric-simulated digesta: 2S albumins are main contributors to the IgE reactivity of short digestion-resistant peptidesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractван, Хаге М.; Ћирковић Величковић, Тања; Станиц-Вуциниц, Драгана; Продић, И.; Aпостоловиц, Д.; Михаиловић, Јелена; Радибратовић, Милица; Радосављевиц, Ј.; Буразер, Л.; Милчић, Милош; Смиљаниц, К.;
dc.citation.volume48
dc.citation.issue6
dc.citation.spage731
dc.citation.epage740
dc.citation.other48(6): 731-740
dc.citation.rankM21
dc.description.otherThis is peer-reviewed version of the following article: Prodic, I.; Stanic-Vucinic, D.; Apostolovic, D.; Mihailovic, J.; Radibratovic, M.; Radosavljevic, J.; Burazer, L.; Milcic, M.; Smiljanic, K.; van Hage, M.; et al. Influence of Peanut Matrix on Stability of Allergens in Gastric-Simulated Digesta: 2S Albumins Are Main Contributors to the IgE Reactivity of Short Digestion-Resistant Peptides. Clinical and Experimental Allergy 2018, 48 (6), 731–740. [https://doi.org/10.1111/cea.13113]
dc.description.otherThe published version: [http://cer.ihtm.bg.ac.rs/handle/123456789/2299]
dc.identifier.pmid29412488
dc.identifier.doi10.1111/cea.13113
dc.identifier.rcubConv_3944
dc.identifier.fulltexthttp://cer.ihtm.bg.ac.rs/bitstream/id/15872/Influence_of_peanut_acc_2018.pdf
dc.identifier.scopus2-s2.0-85043571987
dc.identifier.wos000434080100013
dc.type.versionacceptedVersion


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