Show simple item record

dc.creatorNikolić, Ines
dc.creatorMitsou, Evgenia
dc.creatorPantelić, Ivana
dc.creatorRanđelović, Danijela
dc.creatorMarković, Bojan D.
dc.creatorPapadimitriou, Vassiliki
dc.creatorXenakis, Aristotelis
dc.creatorLunter, Dominique Jasmin
dc.creatorZugic, Ana
dc.creatorSavić, Snežana D.
dc.date.accessioned2019-12-25T11:22:09Z
dc.date.available2019-12-25T11:22:09Z
dc.date.issued2020
dc.identifier.issn09280987
dc.identifier.urihttp://cer.ihtm.bg.ac.rs/handle/123456789/3309
dc.description.abstracthe objective of this work was to develop low-energy nanoemulsions for enhanced dermal delivery of curcumin, using monoterpene compounds eucalyptol (EUC) and pinene (PIN) as chemical penetration enhancers. Spontaneous emulsification was the preparation method. All formulations contained 10% of the oil phase (medium-chain triglycerides (MCT), or their mixture with EUC or PIN). Formulations were stabilized by the combination of polysorbate 80 and soybean lecithin (surfactant-to-oil-ratio=1). Concentration of curcumin was set to 3 mg/ml. Average droplet diameter of all tested formulations ranged from 102 nm to 132 nm, but the ones containing monoterpenes had significantly smaller size compared to the MCT formulation. Such finding was profoundly studied through electron paramagnetic resonance spectroscopy, which proved that the presence of monoterpenes modified the nanoemulsions’ interfacial environment, resulting in droplet size reduction. The release study of curcumin (using Franz cells) demonstrated that the cumulative amount released after 6 h of the experiment was 10.1 ± 0.2% for the MCT nanoemulsions, 13.9 ± 0.1% and 14.0 ± 0.2% for PIN and EUC formulations, respectively. In vivo tape stripping revealed their performances in delivering curcumin into the skin, indicating the following order: EUC>MCT>PIN. The formulation with EUC was clearly the most successful, giving the highest cumulative amount of curcumin that penetrated per surface unit: 34.24±5.68 µg/cm2. The MCT formulation followed (30.62±2.61 µg/cm2) and, finally, the one with PIN (21.61±0.11 µg/cm2). These results corelated with curcumin's solubility in the chosen oils: 4.18±0.02 mg/ml for EUC, 1.67±0.04 mg/ml for MCT and 0.21±0.01 mg/ml for PIN. Probably, higher solubility in the oil phase of the nanoemulsion promoted curcumin's solubility in the superficial skin layers, providing enhanced penetration.en
dc.publisherElsevier BVen
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/34031/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/32008/RS//
dc.relationbilateral project between the Republic of Serbia and the Federal Republic of Germany (Biosurfactants and biopolysaccharides/film-forming polymers as cosmetic raw materials and prospective pharmaceutical excipients: formulation of colloidal and film-forming delivery systems)
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Pharmaceutical Sciencesen
dc.subjectMonoterpene
dc.subjectLow-energy nanoemulsion
dc.subjectElectron paramagnetic resonance spectroscopy
dc.subjectInterfacial dynamics
dc.subjectPenetration
dc.subjectCurcumin
dc.titleMicrostructure and biopharmaceutical performances of curcumin-loaded low-energy nanoemulsions containing eucalyptol and pinene: Terpenes’ role overcome penetration enhancement effect?en
dc.typearticleen
dc.rights.licenseARRen
dcterms.abstractМарковиц, Бојан; Пападимитриоу, Вассилики; Xенакис, Aристотелис; Лунтер, Доминиqуе Јасмин; Николиц, Инес; Зугиц, Aна; Митсоу, Евгениа; Пантелиц, Ивана; Рандјеловиц, Данијела; Савиц, Снезана;
dc.rights.holderElsevier BV
dc.citation.volume142
dc.citation.spage105135
dc.citation.rankM21~
dc.description.otherThe peer-reviewed version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3386]
dc.identifier.doi10.1016/j.ejps.2019.105135
dc.identifier.scopus2-s2.0-85074713831
dc.identifier.wos000505188900020
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record