Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain
Fina strukturna analiza fungalnog polisaharida pululana dobijenog pomoću Aureobasidium pullulans, soj CH-1
Апстракт
The structure of pullulan, the extracellular α-D-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a linear α-D-glucan consisting of maltotriosyl repeat units connected terminally by (1->6)-α-D-glucosidic bonds. Occasionally some of maltotriosyl residues are replaced by higher oligosaccharide units, most frequently with maltotetraosyl residues. Using the susceptibility of pullulan CH-1 (obtained from strain CH-1 of Aureobasidium pullulans) to hydrolysis catalysed by porcine alpha-amylase, the polysaccharide was cleaved and the fragments obtained fractionated by gel-permeation chromatography. The heterogenous size of the fragments indicates that there is no apparent regular distribution of tetrasaccharide units in the pullulan chain. Enzymatic digestion of pullulan CH-1 using pullulanase, followed by gel-permeation chromatography of the resulting digest confirmed these results as did preparative paper chromatography and CI mass spectrometry of the s...eparated components, i.e., that maltotetraosyl units (about 7 %) are building units of pullulan CH-1.
Pululan, ekstracelularni polisaharid koji proizvodi kvascu slična gljiva Aureobasidium pullulans, jeste linearni glukan čiju strukturu pretežno čine polimaltotriozil ostatci povezani međusobno α-(1->6)-glikozidnim vezama. Varijacije u strukturi ovog polisaharida mogu nastati usled zamene maltotrioznih ostataka na pojedinim mestima polimernog lanca oligosaharidnim fragmentima dužeg niza, najčešće maltotetraoznim ostacima. U ovom radu ispitan je pululan koji proizvodi A. pullulans, soj CH-1, enzimskom hidrolizom sa pankreasnom α-amilazom i pululanazom, sa ciljem da se utvrdi da li i u kom odnosu ovaj polisaharid sadrži oligosaharidne fragmente, kao i da se odredi dužina oligosaharidnog niza, odnosno broj monosaharidnih ostataka koji ulaze u njegov sastav. Primenom uobičajenih analitičkih metoda (gel-filtraciona hromatografija, metilaciona analiza, preparativna hromatografija kombinovana sa masenom spektrometrijom metilovanih oligosaharidnih alditola), utvrđeno je da su maltotetraozne jed...inice (oko 7 %) integralni deo polisaharidnog lanca pululana CH-1. Raspored ovih jedinica je nasumičan.
Кључне речи:
Aureobasidium pullulans / polysaccharide / pullulan / amylolysis / pullulanolysisИзвор:
Journal of the Serbian Chemical Society, 2001, 66, 6, 377-383Издавач:
- Serbian Chemical Society
Институција/група
IHTMTY - JOUR AU - Jakovljević, Dragica AU - Vrvić, Miroslav AU - Radulović, Milanka AU - Hranisavljević-Jakovljević, Mirjana PY - 2001 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/33 AB - The structure of pullulan, the extracellular α-D-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a linear α-D-glucan consisting of maltotriosyl repeat units connected terminally by (1->6)-α-D-glucosidic bonds. Occasionally some of maltotriosyl residues are replaced by higher oligosaccharide units, most frequently with maltotetraosyl residues. Using the susceptibility of pullulan CH-1 (obtained from strain CH-1 of Aureobasidium pullulans) to hydrolysis catalysed by porcine alpha-amylase, the polysaccharide was cleaved and the fragments obtained fractionated by gel-permeation chromatography. The heterogenous size of the fragments indicates that there is no apparent regular distribution of tetrasaccharide units in the pullulan chain. Enzymatic digestion of pullulan CH-1 using pullulanase, followed by gel-permeation chromatography of the resulting digest confirmed these results as did preparative paper chromatography and CI mass spectrometry of the separated components, i.e., that maltotetraosyl units (about 7 %) are building units of pullulan CH-1. AB - Pululan, ekstracelularni polisaharid koji proizvodi kvascu slična gljiva Aureobasidium pullulans, jeste linearni glukan čiju strukturu pretežno čine polimaltotriozil ostatci povezani međusobno α-(1->6)-glikozidnim vezama. Varijacije u strukturi ovog polisaharida mogu nastati usled zamene maltotrioznih ostataka na pojedinim mestima polimernog lanca oligosaharidnim fragmentima dužeg niza, najčešće maltotetraoznim ostacima. U ovom radu ispitan je pululan koji proizvodi A. pullulans, soj CH-1, enzimskom hidrolizom sa pankreasnom α-amilazom i pululanazom, sa ciljem da se utvrdi da li i u kom odnosu ovaj polisaharid sadrži oligosaharidne fragmente, kao i da se odredi dužina oligosaharidnog niza, odnosno broj monosaharidnih ostataka koji ulaze u njegov sastav. Primenom uobičajenih analitičkih metoda (gel-filtraciona hromatografija, metilaciona analiza, preparativna hromatografija kombinovana sa masenom spektrometrijom metilovanih oligosaharidnih alditola), utvrđeno je da su maltotetraozne jedinice (oko 7 %) integralni deo polisaharidnog lanca pululana CH-1. Raspored ovih jedinica je nasumičan. PB - Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain T1 - Fina strukturna analiza fungalnog polisaharida pululana dobijenog pomoću Aureobasidium pullulans, soj CH-1 VL - 66 IS - 6 SP - 377 EP - 383 UR - https://hdl.handle.net/21.15107/rcub_cer_33 ER -
@article{ author = "Jakovljević, Dragica and Vrvić, Miroslav and Radulović, Milanka and Hranisavljević-Jakovljević, Mirjana", year = "2001", abstract = "The structure of pullulan, the extracellular α-D-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a linear α-D-glucan consisting of maltotriosyl repeat units connected terminally by (1->6)-α-D-glucosidic bonds. Occasionally some of maltotriosyl residues are replaced by higher oligosaccharide units, most frequently with maltotetraosyl residues. Using the susceptibility of pullulan CH-1 (obtained from strain CH-1 of Aureobasidium pullulans) to hydrolysis catalysed by porcine alpha-amylase, the polysaccharide was cleaved and the fragments obtained fractionated by gel-permeation chromatography. The heterogenous size of the fragments indicates that there is no apparent regular distribution of tetrasaccharide units in the pullulan chain. Enzymatic digestion of pullulan CH-1 using pullulanase, followed by gel-permeation chromatography of the resulting digest confirmed these results as did preparative paper chromatography and CI mass spectrometry of the separated components, i.e., that maltotetraosyl units (about 7 %) are building units of pullulan CH-1., Pululan, ekstracelularni polisaharid koji proizvodi kvascu slična gljiva Aureobasidium pullulans, jeste linearni glukan čiju strukturu pretežno čine polimaltotriozil ostatci povezani međusobno α-(1->6)-glikozidnim vezama. Varijacije u strukturi ovog polisaharida mogu nastati usled zamene maltotrioznih ostataka na pojedinim mestima polimernog lanca oligosaharidnim fragmentima dužeg niza, najčešće maltotetraoznim ostacima. U ovom radu ispitan je pululan koji proizvodi A. pullulans, soj CH-1, enzimskom hidrolizom sa pankreasnom α-amilazom i pululanazom, sa ciljem da se utvrdi da li i u kom odnosu ovaj polisaharid sadrži oligosaharidne fragmente, kao i da se odredi dužina oligosaharidnog niza, odnosno broj monosaharidnih ostataka koji ulaze u njegov sastav. Primenom uobičajenih analitičkih metoda (gel-filtraciona hromatografija, metilaciona analiza, preparativna hromatografija kombinovana sa masenom spektrometrijom metilovanih oligosaharidnih alditola), utvrđeno je da su maltotetraozne jedinice (oko 7 %) integralni deo polisaharidnog lanca pululana CH-1. Raspored ovih jedinica je nasumičan.", publisher = "Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society", title = "Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain, Fina strukturna analiza fungalnog polisaharida pululana dobijenog pomoću Aureobasidium pullulans, soj CH-1", volume = "66", number = "6", pages = "377-383", url = "https://hdl.handle.net/21.15107/rcub_cer_33" }
Jakovljević, D., Vrvić, M., Radulović, M.,& Hranisavljević-Jakovljević, M.. (2001). Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain. in Journal of the Serbian Chemical Society Serbian Chemical Society., 66(6), 377-383. https://hdl.handle.net/21.15107/rcub_cer_33
Jakovljević D, Vrvić M, Radulović M, Hranisavljević-Jakovljević M. Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain. in Journal of the Serbian Chemical Society. 2001;66(6):377-383. https://hdl.handle.net/21.15107/rcub_cer_33 .
Jakovljević, Dragica, Vrvić, Miroslav, Radulović, Milanka, Hranisavljević-Jakovljević, Mirjana, "Fine structural analysis of the fungal polysaccharide pullulan elaborated by Aureobasidium pullulans, CH-1 strain" in Journal of the Serbian Chemical Society, 66, no. 6 (2001):377-383, https://hdl.handle.net/21.15107/rcub_cer_33 .
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