The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer.
Japan International Cooperation Agency (JICA) grassroot project “Capacity building for analysis and reduction measures of persistent organic pollutants in Serbia”
TY - CONF
AU - Žerađanin, Aleksandra
AU - Gojgić-Cvijović, Gordana
AU - Jakovljević, Dragica
AU - Lončarević, Branka
AU - Vrvić, Miroslav
AU - Beškoski, Vladimir
PY - 2017
UR - http://www.bds.org.rs/en/conferences.php
UR - http://cer.ihtm.bg.ac.rs/handle/123456789/3283
AB - The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer.
PB - Faculty of Chemistry, Serbian Biochemical Society
C3 - Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings
T1 - Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3
ER -
@conference{
author = "Žerađanin, Aleksandra and Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Lončarević, Branka and Vrvić, Miroslav and Beškoski, Vladimir",
year = "2017",
url = "http://www.bds.org.rs/en/conferences.php, http://cer.ihtm.bg.ac.rs/handle/123456789/3283",
abstract = "The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings",
title = "Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3"
}
Žerađanin, A., Gojgić-Cvijović, G., Jakovljević, D., Lončarević, B., Vrvić, M.,& Beškoski, V. (2017). Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3.
Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings
Faculty of Chemistry, Serbian Biochemical Society..
Žerađanin A, Gojgić-Cvijović G, Jakovljević D, Lončarević B, Vrvić M, Beškoski V. Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings. 2017;
Žerađanin Aleksandra, Gojgić-Cvijović Gordana, Jakovljević Dragica, Lončarević Branka, Vrvić Miroslav, Beškoski Vladimir, "Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3" Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings (2017)
