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Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3

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2017
05_biochem2017.pdf (394.9Kb)
Authors
Žerađanin, Aleksandra
Gojgić-Cvijović, Gordana
Jakovljević, Dragica
Lončarević, Branka
Vrvić, Miroslav
Beškoski, Vladimir
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Abstract
The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer.
Keywords:
levan-type polysaccharide / exopolysaccharides / GC-MS / NMR
Source:
Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings, 2017
Publisher:
  • Faculty of Chemistry, Serbian Biochemical Society
Funding / projects:
  • Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
  • Japan International Cooperation Agency (JICA) grassroot project “Capacity building for analysis and reduction measures of persistent organic pollutants in Serbia”
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_cer_3283
URI
http://www.bds.org.rs/en/conferences.php
https://cer.ihtm.bg.ac.rs/handle/123456789/3283
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IHTM
TY  - CONF
AU  - Žerađanin, Aleksandra
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Lončarević, Branka
AU  - Vrvić, Miroslav
AU  - Beškoski, Vladimir
PY  - 2017
UR  - http://www.bds.org.rs/en/conferences.php
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3283
AB  - The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings
T1  - Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3
UR  - https://hdl.handle.net/21.15107/rcub_cer_3283
ER  - 
@conference{
author = "Žerađanin, Aleksandra and Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Lončarević, Branka and Vrvić, Miroslav and Beškoski, Vladimir",
year = "2017",
abstract = "The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings",
title = "Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3",
url = "https://hdl.handle.net/21.15107/rcub_cer_3283"
}
Žerađanin, A., Gojgić-Cvijović, G., Jakovljević, D., Lončarević, B., Vrvić, M.,& Beškoski, V.. (2017). Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings
Faculty of Chemistry, Serbian Biochemical Society..
https://hdl.handle.net/21.15107/rcub_cer_3283
Žerađanin A, Gojgić-Cvijović G, Jakovljević D, Lončarević B, Vrvić M, Beškoski V. Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings. 2017;.
https://hdl.handle.net/21.15107/rcub_cer_3283 .
Žerađanin, Aleksandra, Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Lončarević, Branka, Vrvić, Miroslav, Beškoski, Vladimir, "Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3" in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings (2017),
https://hdl.handle.net/21.15107/rcub_cer_3283 .

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