Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3
Authors
Žerađanin, Aleksandra
Gojgić-Cvijović, Gordana

Jakovljević, Dragica

Lončarević, Branka

Vrvić, Miroslav

Beškoski, Vladimir

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The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer.
Keywords:
levan-type polysaccharide / exopolysaccharides / GC-MS / NMRSource:
Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings, 2017Publisher:
- Faculty of Chemistry, Serbian Biochemical Society
Funding / projects:
- Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products (RS-43004)
- Japan International Cooperation Agency (JICA) grassroot project “Capacity building for analysis and reduction measures of persistent organic pollutants in Serbia”
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IHTMTY - CONF AU - Žerađanin, Aleksandra AU - Gojgić-Cvijović, Gordana AU - Jakovljević, Dragica AU - Lončarević, Branka AU - Vrvić, Miroslav AU - Beškoski, Vladimir PY - 2017 UR - http://www.bds.org.rs/en/conferences.php UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3283 AB - The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer. PB - Faculty of Chemistry, Serbian Biochemical Society C3 - Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings T1 - Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3 UR - https://hdl.handle.net/21.15107/rcub_cer_3283 ER -
@conference{ author = "Žerađanin, Aleksandra and Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Lončarević, Branka and Vrvić, Miroslav and Beškoski, Vladimir", year = "2017", abstract = "The aim of this work was structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. For the structural instrumental analysis of EPS, the following methods were applied: GC-MS (gas chromatography mass spectrometry) and correlated two-dimensional NMR (nuclear magnetic resonance) techniques - DEPT 135 (distortionless enhancement by polarization transfer), COSY (correlation spectroscopy), and HSQC (heteronuclear single quantum coherence). Methylation was performed by the method which described earlier. The permethylated EPS was subjected to reductive cleavage as described by Rolf and Gray. Cleaved monomer units were acetylated. Obtained acetylated, methylated products were analyzed by GC-MS. These analyses were performed on a GCxGC-MS (Shimadzu, Kyoto, Japan). NMR spectra of the isolated EPS were measured on a Bruker AVANCE III 500 spectrometer.", publisher = "Faculty of Chemistry, Serbian Biochemical Society", journal = "Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings", title = "Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3", url = "https://hdl.handle.net/21.15107/rcub_cer_3283" }
Žerađanin, A., Gojgić-Cvijović, G., Jakovljević, D., Lončarević, B., Vrvić, M.,& Beškoski, V.. (2017). Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings Faculty of Chemistry, Serbian Biochemical Society.. https://hdl.handle.net/21.15107/rcub_cer_3283
Žerađanin A, Gojgić-Cvijović G, Jakovljević D, Lončarević B, Vrvić M, Beškoski V. Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings. 2017;. https://hdl.handle.net/21.15107/rcub_cer_3283 .
Žerađanin, Aleksandra, Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Lončarević, Branka, Vrvić, Miroslav, Beškoski, Vladimir, "Structural characterization of EPS produced by Brachybacterium paraconglomeratum sp. CH-KOV3" in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings (2017), https://hdl.handle.net/21.15107/rcub_cer_3283 .