Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cellulase (EC 3.2.1.4) are important enzymes in food, paper, textile, detergent and biofuel industries. Most cellulases have low activity and stability. Improving these properties would have substantial impact on numerous industrial processes. Enzymatic properties can be improved by directed evolution, but the screening process is the limiting step. Coupled cellulase assay has been developed in order to improve the screening process. This method does not require boiling samples and allows rapid screening of mutants in a microtiter plate. The aim of this study was to establish enzyme coupled assay where cellulase first hydrolyzes carboxymethylcellulose (CMC), and cellobioses dehydrogenase (CBDH) and dichlorophenolindophenol (DCPIP) is used subsequently for detection of reducing ends.
Кључне речи:
Cellulase / semi-rational design / mutationsИзвор:
Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings, 2017Издавач:
- Faculty of Chemistry, Serbian Biochemical Society
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-46010)
- Нове технологије за мониторинг и заштиту животног окружења од штетних хемијских супстанци и радијационог оптерећења (RS-43009)
Институција/група
IHTMTY - CONF AU - Zelenović, Jelena AU - Ostafe, Raluca AU - Fischer, Rainer AU - Prodanović, Radivoje PY - 2017 UR - http://www.bds.org.rs/en/conferences.php UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3282 AB - Cellulase (EC 3.2.1.4) are important enzymes in food, paper, textile, detergent and biofuel industries. Most cellulases have low activity and stability. Improving these properties would have substantial impact on numerous industrial processes. Enzymatic properties can be improved by directed evolution, but the screening process is the limiting step. Coupled cellulase assay has been developed in order to improve the screening process. This method does not require boiling samples and allows rapid screening of mutants in a microtiter plate. The aim of this study was to establish enzyme coupled assay where cellulase first hydrolyzes carboxymethylcellulose (CMC), and cellobioses dehydrogenase (CBDH) and dichlorophenolindophenol (DCPIP) is used subsequently for detection of reducing ends. PB - Faculty of Chemistry, Serbian Biochemical Society C3 - Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings T1 - Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase UR - https://hdl.handle.net/21.15107/rcub_cer_3282 ER -
@conference{ author = "Zelenović, Jelena and Ostafe, Raluca and Fischer, Rainer and Prodanović, Radivoje", year = "2017", abstract = "Cellulase (EC 3.2.1.4) are important enzymes in food, paper, textile, detergent and biofuel industries. Most cellulases have low activity and stability. Improving these properties would have substantial impact on numerous industrial processes. Enzymatic properties can be improved by directed evolution, but the screening process is the limiting step. Coupled cellulase assay has been developed in order to improve the screening process. This method does not require boiling samples and allows rapid screening of mutants in a microtiter plate. The aim of this study was to establish enzyme coupled assay where cellulase first hydrolyzes carboxymethylcellulose (CMC), and cellobioses dehydrogenase (CBDH) and dichlorophenolindophenol (DCPIP) is used subsequently for detection of reducing ends.", publisher = "Faculty of Chemistry, Serbian Biochemical Society", journal = "Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings", title = "Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase", url = "https://hdl.handle.net/21.15107/rcub_cer_3282" }
Zelenović, J., Ostafe, R., Fischer, R.,& Prodanović, R.. (2017). Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings Faculty of Chemistry, Serbian Biochemical Society.. https://hdl.handle.net/21.15107/rcub_cer_3282
Zelenović J, Ostafe R, Fischer R, Prodanović R. Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase. in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings. 2017;. https://hdl.handle.net/21.15107/rcub_cer_3282 .
Zelenović, Jelena, Ostafe, Raluca, Fischer, Rainer, Prodanović, Radivoje, "Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase" in Serbian Biochemical Society Seventh Conference "Biochemistry of Control in Life and Technology" - Proceedings (2017), https://hdl.handle.net/21.15107/rcub_cer_3282 .