Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents
Authors
Milojkov, Dušan V.Silvestre, Oscar F.
Stanić, Vojislav Dj.

Janjić, Goran

Mutavdžić, Dragosav

Milanović, Marija
Nieder, Jana B.
Article (Accepted Version)

Elsevier
Metadata
Show full item recordAbstract
Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Addit...ionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.
Keywords:
Fluorapatite nanocrystals / Praseodymium / Bioimaging / Cancer cellsSource:
Journal of Luminescence, 2020, 217, 116757-Publisher:
- Elsevier
Funding / projects:
- CCDR-N: NORTE-01-0145-FEDER-000019
- Marie Curie fellowship, EU-EC, COFUND programme: NanoTRAINforGrowth (600375)
- Advanced technologies for monitoring and environmental protection from chemical pollutants and radiation burden (RS-43009)
- Synthesis of nanopowders and processing of ceramics and nanocomposites with specific electric and magnetic properties for application in integrated passive components (RS-45021)
- Studies of enzyme interactions with toxic and pharmacologically active molecules (RS-172023)
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)
Note:
- This is the peer-reviewed version of the manuscript: Journal of Luminescence, 2020, 217, 116757 https://doi.org/10.1016/j.jlumin.2019.116757
- The published version: http://cer.ihtm.bg.ac.rs/handle/123456789/3275
DOI: 10.1016/j.jlumin.2019.116757
ISSN: 0022-2313
WoS: 000501362400035
Scopus: 2-s2.0-85073162138
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Institution/Community
IHTMTY - JOUR AU - Milojkov, Dušan V. AU - Silvestre, Oscar F. AU - Stanić, Vojislav Dj. AU - Janjić, Goran AU - Mutavdžić, Dragosav AU - Milanović, Marija AU - Nieder, Jana B. PY - 2020 UR - https://cer.ihtm.bg.ac.rs/handle/123456789/3276 AB - Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications. PB - Elsevier T2 - Journal of Luminescence T1 - Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents VL - 217 SP - 116757 DO - 10.1016/j.jlumin.2019.116757 ER -
@article{ author = "Milojkov, Dušan V. and Silvestre, Oscar F. and Stanić, Vojislav Dj. and Janjić, Goran and Mutavdžić, Dragosav and Milanović, Marija and Nieder, Jana B.", year = "2020", abstract = "Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.", publisher = "Elsevier", journal = "Journal of Luminescence", title = "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents", volume = "217", pages = "116757", doi = "10.1016/j.jlumin.2019.116757" }
Milojkov, D. V., Silvestre, O. F., Stanić, V. Dj., Janjić, G., Mutavdžić, D., Milanović, M.,& Nieder, J. B.. (2020). Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence Elsevier., 217, 116757. https://doi.org/10.1016/j.jlumin.2019.116757
Milojkov DV, Silvestre OF, Stanić VD, Janjić G, Mutavdžić D, Milanović M, Nieder JB. Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence. 2020;217:116757. doi:10.1016/j.jlumin.2019.116757 .
Milojkov, Dušan V., Silvestre, Oscar F., Stanić, Vojislav Dj., Janjić, Goran, Mutavdžić, Dragosav, Milanović, Marija, Nieder, Jana B., "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents" in Journal of Luminescence, 217 (2020):116757, https://doi.org/10.1016/j.jlumin.2019.116757 . .